STAFF PRIORITY TIME CRYSTAL STRUCTURE DETERMINATION OF PNGASE F

多肽-N4-(N-乙酰-b-D-氨基葡萄糖基)天冬酰胺酰胺酶F是一种 脑膜败血黄杆菌的34 kDa氨基水解酶 糖蛋白中完整的天冬酰胺连接的寡糖。高 分辨率晶体数据(1.6 E及更高)收集于 三个突变体和天然酶与产物形成复合体， 二-N-乙酰基壳二糖。该模型目前正在针对 数据集。所有模型的R因子都在18%左右。高潮 分辨结构显示了酶与其结合的清晰细节 并为其产品的进一步设计提供了新的起点 实验旨在探讨该酶的作用机理。

Peptide-N4-(N-acetyl-b-D-glucosaminyl) asparagine amidase F is a 34kDa aminohydrolase from Flavobacterium meningosepticum that removes intact asparagine-linked oligosaccharides from glycoproteins. High resolution crystallographic data (1.6 E and better) were collected on three mutants and the native enzyme in complex with the product, di-N-acetylchitobiose. The model is currently being refined against the data sets. The R-factors of all models are around 18%. The high resolution structures show clear details of binding of the enzyme to its product and provide new starting points for the design of further experiments aimed at the investigation of the mechanism of the enzyme.