CYTOCHROME BC1 REDUCTASE

细胞色素 BC1 还原酶

• 批准号: 6586592
• 负责人: JOONG W LEE
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586592
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

Cytochrome bc1 reductase is an integral membrane protein complex, over 240 kDa in weight and composed of 11 different subunits. Biologically, this complex is quite important since it is a key link in the mitochondrial respiratory cycle, coupling the electron transfer from ubiquinol to cytochrome c with the formation of the proton gradient across the inner mitochondrial membrane. The complexity as well as the rather large size of the protein complex requires the use of synchrotron sources for all data collection and screening studies. We are requesting beam time on beamline 1-5 for screening of heavy atom derivatives. This beamline is perfectly suited for this purpose with its capacity to go to long camera distances of more than 500 mm. It also has a tunable wavelength source so that we can maximize the anomalous signal for the different heavy atom derivatives.

细胞色素bc 1还原酶是一种完整的膜蛋白复合物， 分子量超过240 kDa，由11种不同的亚基组成。 在生物学上，这个复合体是非常重要的，因为它是一个关键环节， 在线粒体呼吸循环中， 从泛醇到细胞色素c， 线粒体内膜的梯度。 复杂性作为 由于蛋白质复合物的尺寸相当大， 同步辐射源的所有数据收集和筛选研究。 我们请求1-5号光束线的光束时间， 原子衍生物 这条光束线非常适合这个目的 它能够进行超过500 mm的长距离摄像。 它还有一个可调波长光源，这样我们就可以最大限度地 不同重原子衍生物的异常信号。