HUMAN REPLICATION PROTEIN COMPLEXED W/ SINGLE STRANDED DNA

复制蛋白A（RPA）是DNA的关键组成部分 复制和修复机器。 RPA是单链DNA 与DNA复制起点结合相互作用的结合蛋白 蛋白质、DNA聚合酶、转录激活蛋白和DNA 修复因子，包括那些以几种形式突变的修复因子， 人类癌症 RPA是异源三聚体; DNA结合结构域已被 定位于最大的亚基。 我们用有限的蛋白水解 和缺失分析以鉴定最小单链DNA 结合域 我们已经将这个结构域与ssDNA一起结晶化。 我们正在对我们的单位进行重原子搜索， 用于共结晶的硒代甲硫氨酸标记的蛋白质。

Replication protein A (RPA) is a key component of the DNA replication and repair machineries. RPA is a single stranded DNA binding protein that interacts with DNA replication origin binding proteins, DNA polymerases, transcription activator proteins and DNA repair factors, including those that are mutated in several forms of human cancer. RPA is a heterotrimer; the DNA binding domain has been localized to the largest subunit. We have used limited proteolysis and deletion analysis to identify the minimal single strand DNA binding domain. We have crystallized this domain together with ssDNA. We are pursuing heavy atom searches on our unit and have also prepared selenomethionine-labeled protein for co-crystallization.