XRAY DIFFRACTION ANALYSES OF EF TU COMPLEXES & PECTATE LYASE COMPLEXES

EF TU 复合物的 X 射线衍射分析

• 批准号: 6586750
• 负责人: ROBERT SCAVETTA
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586750
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; genome; proteins; radiography; structural biology

HK97 are mature protein particles, assembled as provirions in an E. coli system expressing the 42kD capsid protein of HK97 and induced to maturation by exposure to 6M urea. To our knowledge these are the first crystals of a dsDNA phage that diffract x-rays. Our need for synchrotron radiation results from the low intrinsic scattering from such a large unit cell (and the relatively few number of them in the crystal), thus the intensity is required to give us the highest resolution patterns.

HK97是成熟的蛋白质颗粒，以前病毒的形式组装在 表达HK97 42kD衣壳蛋白的大肠杆菌系统及诱导表达 通过暴露在6M尿素中成熟。据我们所知，这些是 DsDNA噬菌体的第一个晶体，可以绕射x射线。我们需要的是 同步辐射是由低本征散射引起的 如此大的单元格(以及它们在 水晶)，因此需要强度来给我们最高 分辨率模式。