Long Wavelength Quantum Dot-based probes - cell tracking

描述(由申请人提供)：我们将开发新技术以供使用 量子点在体外生物成像中的应用。在一个不断发展的计划中，量子 DOT技术将专门用于(I)细胞识别和 跟踪组织中的细胞，(2)通过多个 组织中的世代(3)血管定位和(4)深层3-D成像 QDot标记的聚合物支架结构相对于QDot标记的细胞 工程化组织模型。量子点公司将准备新型的 具有适合于对细胞和结构进行深度成像的性质的量子点 纸巾。将特别强调用于成像的近红外量子点 更深的组织中。卡内基梅隆大学的科学技术中心 然后，大学将开发方法来派生现有的和新的Q点 在细胞生物学中的应用。STC最初将使用可用Q点和 结合，通过各种方法标记细胞，然后测试细胞是否 荧光亮度、标记稳定性、细胞存活和功能。 随着较新的量子点问世，它们将接受类似的测试和 与现有的QDot和现有的有机荧光探针相比， Alexa染料和菁染料。荧光寿命成像能力将是 添加到STC中的3-D光栅成像器，以增强信号与背景的关系 在深部组织成像中利用相对较长的发射 Qdots的一生。为了获得对开发计划的反馈，我们拥有 包括与最近成立的骨组织工程中心的合作 在卡内基梅隆大学。在此协作中，我们将研究 发展中的量子点技术在细胞定位、运动研究中的应用 以及工程化骨组织的三维结构中的增殖。这是一个 特别具有挑战性和相关性的系统，需要QDot技术 将细胞跟踪扩展到更密集和更高散射的组织基质， 包括含羟基磷灰石的人工骨基质。根据结论 对于这个项目，我们希望有仪器和探测器来执行 在毫米深度的时间分辨率多色成像在许多自然和 人造组织。因此，该技术将是通用的，并在 许多生物和医学应用。

DESCRIPTION (provided by applicant): We will develop new technologies to employ quantum dots in biological imaging in vitro. In an evolving program, quantum dot technology will be developed specifically for (I) cell identification and tracking of cells in tissues, (2) tracking cell proliferation through multiple generations in tissues (3) vasculature location, and (4) deep 3-D imaging of Qdot-labeled polymer scaffold structures relative to the Qdot-labeled cells in engineered tissue models. Quantum Dots Corporation will prepare new types of Qdots that have properties suited to deep imaging of cells and structures in tissues. Particular emphasis will be placed on near infrared Qdots for imaging deeper in tissues. The Science and Technology Center at Carnegie Mellon University will then develop methods to derivatize Qdots for existing and new applications in cell biology. The STC will initially use available Qdots and conjugates, label cells by a variety of means, then test the cells for fluorescent brightness, stability of labeling, and cell survival and function. As newer quantum dots become available, they will be similarly tested and compared with existing Qdots and existing organic fluorescent probes, e.g., Alexa dyes and cyanine dyes. Fluorescence lifetime imaging capability will be added to the 3-D grating imager in the STC for enhancing signal-to-background in deep tissue imaging by taking advantage of the relatively long emission lifetime of Qdots. To obtain feedback for the development program we have included collaboration with the recently formed Bone Tissue Engineering Center at Carnegie Mellon University. In this collaboration we will examine the utility of the developing Qdot technology for studying cell location, movement and proliferation in the 3-D structures of engineered bone tissue. This is a particularly challenging and relevant system that requires Qdot technology to extend cell tracking to denser and more highly scattering tissue matrices, including hydroxyapatite-containing artificial bone matrices. By the conclusion of this project, we expect to have instrumentation and probes to perform time-resolved multicolor imaging at millimeter depth in many natural and artificial tissues. Thus the technology will be generic and have utility in many biological and medical applications.