Zeiss LSM510 Meta DuoScan Spectral Confocal Microscope

描述(由申请人提供)：本建议书代表一组PHS资助的研究人员申请购买卡尔蔡司LSM 510 Meta DuoScan激光扫描共聚焦显微镜，他们有重叠的成像需求。该系统将由分子生物传感器和成像中心(MBIC)作为卡内基梅隆大学梅隆理工学院的多用户设施来容纳和操作。MBIC目前是仅有的五个国家网络和路径技术中心之一，其核心任务是开发用于检测活细胞和生物组织内分子水平相互作用的光学生物传感器和成像信息学技术。收购拟议的仪器将提供对梅隆研究所最先进的共焦显微镜仪器的现场访问，这对于各种活细胞实验以及MBIC开发的荧光探针技术的常规分析和表征是必不可少的。MBIC和合作伙伴教员的研究实验室中现有的仪器既陈旧又无人支持，或目前已得到充分利用。虽然在匹兹堡一般地区可以使用其他先进的显微镜仪器，但我们发现，这给活细胞和动物实验带来了后勤问题，对于常规分析新的生物传感器和探针技术是不现实的。此外，拟议系统的能力满足了一些现有工具无法满足的当前需求。由于MBIC是一个多学科合作中心，汇集了卡内基梅隆大学多个学术部门、学院和研究机构的不同教职员工，收购的仪器将为整个大学的研究人员提供显著改进的成像能力，以整合到他们的研究计划中，并随后将提供使用先进的显微镜和成像方法解决基本生物学问题的新机会。

DESCRIPTION (provided by applicant): This proposal represents a request from a group of PHS funded investigators having overlapping imaging needs for funds to purchase a Carl Zeiss LSM 510 Meta DuoScan laser scanning confocal microscope. The system will be housed and operated by the Molecular Biosensor and Imaging Center (MBIC) as a multi-user facility in the Mellon College of Science at Carnegie Mellon University. MBIC is currently funded as one of only five National Technology Centers for Networks and Pathways with the core mission to develop optical biosensor and imaging informatics technologies for the detection of molecular-level interactions within living cells and biological tissues. The acquisition of the proposed instrument would provide on-site access to state of the art confocal microscopy instrumentation within the Mellon Institute, which is essential for a variety of live cell experiments as well as for routine analysis and characterization of the fluorescent probe technology developed within MBIC. Current instrumentation available within MBIC and partner faculty's research laboratories are both aged and unsupported or are currently fully utilized. While access to other advanced microscopy instrumentation is possible within the general Pittsburgh area, we have found that this poses a logistical problem for experiments in live cells and animals and is not practical for analyses of new biosensor and probe technologies on a routine basis. In addition, the capabilities of the proposed system meet a number of current needs that existing instrumentation is incapable of fulfilling. Since MBIC is a multi-disciplinary collaborative center which brings together a diverse collection of Carnegie Mellon faculty spanning several academic departments, colleges and research institutes, the acquired instrument will provide investigators across the university with substantially improved imaging capabilities to integrate into their research programs and will subsequently provide new opportunities to address fundamental biological questions using advanced microscopy and imaging methodologies.