Zeiss LSM510 Meta DuoScan Spectral Confocal Microscope

描述（由申请人提供）：本提案代表了一组PHS资助的研究者的请求，这些研究者对购买Carl Zeiss LSM 510 Meta DuoScan激光扫描共聚焦显微镜的资金有重叠的成像需求。该系统将由卡内基梅隆大学梅隆科学学院的分子生物传感器和成像中心（MBIC）作为多用户设施进行安置和操作。MBIC目前是仅有的五个网络和路径国家技术中心之一，其核心使命是开发用于检测活细胞和生物组织内分子水平相互作用的光学生物传感器和成像信息学技术。拟议的仪器的收购将提供现场访问的最先进的共聚焦显微镜仪器在梅隆研究所，这是必不可少的各种活细胞实验以及常规分析和表征的荧光探针技术内开发的MBIC。目前MBIC和合作伙伴教师的研究实验室内可用的仪器都老化和不支持或目前充分利用。虽然访问其他先进的显微镜仪器是可能的一般匹兹堡地区内，我们发现，这对活细胞和动物的实验提出了后勤问题，是不实际的分析新的生物传感器和探针技术的常规基础上。此外，所提出的系统的能力满足了现有仪器无法满足的一些当前需求。由于MBIC是一个多学科的合作中心，汇集了卡内基梅隆大学跨越几个学术部门，学院和研究机构的教师，获得的仪器将为整个大学的研究人员提供大大改进的成像能力，以整合到他们的研究计划中，并将随后提供新的机会，利用先进的显微镜和成像技术来解决基本的生物学问题方法论。

DESCRIPTION (provided by applicant): This proposal represents a request from a group of PHS funded investigators having overlapping imaging needs for funds to purchase a Carl Zeiss LSM 510 Meta DuoScan laser scanning confocal microscope. The system will be housed and operated by the Molecular Biosensor and Imaging Center (MBIC) as a multi-user facility in the Mellon College of Science at Carnegie Mellon University. MBIC is currently funded as one of only five National Technology Centers for Networks and Pathways with the core mission to develop optical biosensor and imaging informatics technologies for the detection of molecular-level interactions within living cells and biological tissues. The acquisition of the proposed instrument would provide on-site access to state of the art confocal microscopy instrumentation within the Mellon Institute, which is essential for a variety of live cell experiments as well as for routine analysis and characterization of the fluorescent probe technology developed within MBIC. Current instrumentation available within MBIC and partner faculty's research laboratories are both aged and unsupported or are currently fully utilized. While access to other advanced microscopy instrumentation is possible within the general Pittsburgh area, we have found that this poses a logistical problem for experiments in live cells and animals and is not practical for analyses of new biosensor and probe technologies on a routine basis. In addition, the capabilities of the proposed system meet a number of current needs that existing instrumentation is incapable of fulfilling. Since MBIC is a multi-disciplinary collaborative center which brings together a diverse collection of Carnegie Mellon faculty spanning several academic departments, colleges and research institutes, the acquired instrument will provide investigators across the university with substantially improved imaging capabilities to integrate into their research programs and will subsequently provide new opportunities to address fundamental biological questions using advanced microscopy and imaging methodologies.