Zeiss LSM510 Meta DuoScan Spectral Confocal Microscope

蔡司 LSM510 Meta DuoScan 光谱共焦显微镜

• 批准号: 7390056
• 负责人: Alan S. Waggoner
• 金额: $ 50万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7390056
• 关键词: Address; Animals; Area; Arts; Biological; Biosensor; Cells; Collection; Confocal Microscopy; Detection; Faculty; Fluorescent Probes; Funding; Housing; Image; Institutes; Laboratory Research; Lasers; Life; Methodology; Microscope; Microscopy; Mission; Molecular; Numbers; Optics; Pathway interactions; Research; Research Institute; Research Personnel; Scanning; Science; Site; System; Technology; Tissues; Universities; aged; base; college; imaging informatics; improved; instrument; instrumentation; programs; research study

DESCRIPTION (provided by applicant): This proposal represents a request from a group of PHS funded investigators having overlapping imaging needs for funds to purchase a Carl Zeiss LSM 510 Meta DuoScan laser scanning confocal microscope. The system will be housed and operated by the Molecular Biosensor and Imaging Center (MBIC) as a multi-user facility in the Mellon College of Science at Carnegie Mellon University. MBIC is currently funded as one of only five National Technology Centers for Networks and Pathways with the core mission to develop optical biosensor and imaging informatics technologies for the detection of molecular-level interactions within living cells and biological tissues. The acquisition of the proposed instrument would provide on-site access to state of the art confocal microscopy instrumentation within the Mellon Institute, which is essential for a variety of live cell experiments as well as for routine analysis and characterization of the fluorescent probe technology developed within MBIC. Current instrumentation available within MBIC and partner faculty's research laboratories are both aged and unsupported or are currently fully utilized. While access to other advanced microscopy instrumentation is possible within the general Pittsburgh area, we have found that this poses a logistical problem for experiments in live cells and animals and is not practical for analyses of new biosensor and probe technologies on a routine basis. In addition, the capabilities of the proposed system meet a number of current needs that existing instrumentation is incapable of fulfilling. Since MBIC is a multi-disciplinary collaborative center which brings together a diverse collection of Carnegie Mellon faculty spanning several academic departments, colleges and research institutes, the acquired instrument will provide investigators across the university with substantially improved imaging capabilities to integrate into their research programs and will subsequently provide new opportunities to address fundamental biological questions using advanced microscopy and imaging methodologies.

描述（由申请人提供）：本提案代表了一组小公共卫生服务资助的研究人员的请求，他们有重叠的成像需求，需要资金购买卡尔蔡司LSM 510 Meta duscan激光扫描共聚焦显微镜。该系统将由分子生物传感器和成像中心（MBIC）作为卡内基梅隆大学梅隆科学学院的多用户设施进行安装和操作。MBIC目前是仅有的五个国家网络和通路技术中心之一，其核心任务是开发用于检测活细胞和生物组织内分子水平相互作用的光学生物传感器和成像信息学技术。该仪器的收购将提供梅隆研究所最先进的共聚焦显微镜仪器的现场访问，这对于各种活细胞实验以及MBIC开发的荧光探针技术的常规分析和表征至关重要。目前MBIC和合作教师的研究实验室中可用的仪器要么老化，要么没有支持，要么目前充分利用。虽然在匹兹堡地区可以使用其他先进的显微镜仪器，但我们发现这给活细胞和动物实验带来了后勤问题，并且对于常规的新生物传感器和探针技术的分析是不切实际的。此外，所建议的系统的功能满足现有仪器无法满足的许多当前需求。由于MBIC是一个多学科合作中心，汇集了卡内基梅隆大学多个学术部门、学院和研究机构的各种教职员工，获得的仪器将为整个大学的研究人员提供大大提高的成像能力，以整合到他们的研究项目中，并随后为使用先进的显微镜和成像方法解决基础生物学问题提供新的机会。

项目成果

Assessing the critical period for Rho kinase activity during Drosophila ventral furrow formation.

评估果蝇腹侧沟形成过程中 Rho 激酶活性的关键期。

• DOI: 10.1002/dvdy.23859
• 发表时间: 2012
• 期刊: Developmental dynamics : an official publication of the American Association of Anatomists
• 作者: Krajcovic,MelissaM;Minden,JonathanS
• 通讯作者: Minden,JonathanS