Flourescent Probes & Imaging - Networks & Pathways(RMI)

荧光探针

• 批准号: 7689529
• 负责人: Alan S. Waggoner
• 金额: $ 20.36万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7689529
• 关键词: Automobile Driving; Avidity; Back; Binding; Biology; Biosensor; Biotechnology; Cells; Chemistry; Dyes; Feedback; Fluorescent Dyes; Fluorescent Probes; Genetic; Goals; Imaging technology; Label; Life; Pathway interactions; Pattern; Performance; Pharmacologic Substance; Program Development; Proteins; Protocols documentation; Range; Research; Research Infrastructure; Resolution; Technology; Testing; Time; Training; Universities; cytotoxicity; design; desire; drug discovery; experience; feeding; imaging informatics; imaging probe; macromolecule; nucleic acid structure; programs; protein structure; technology development; tool

We propose to form a nationally visible and responsive center focused on fluorescent probe and imaging technologies for investigating pathways and networks in real time and at high resolution in living cells. The proposed Center will be formed by combining the experience and infrastructure of two already existing Centers in Pittsburgh. The research component of the Center will create a powerful toolbox of intracellular fluorescent labels and biosensors that can be used to study many, if not all, the proteins in pathways and networks of living cells. The strong fluorescent probe development program blends genetics, protein structure, nucleic acid structure and fluorescent dye chemistry. The probes we propose to generate will be genetically expressible so that exogenous macromolecules will not have to be transported into the living cells to be studied. Four superb "Driving Biology Projects" are to be part of the overall Center program in addition to the Technology Development Core. These projects are located at the University of Pittsburgh, Carnegie Mellon, Stanford and Berkeley. Their responsibility is to assist the new center in identifying the optimal technologies for development and for providing crucial feedback regarding the utility of the technologies. Imaging and informatics technologies are integrated with the new fluorescent probe technologies so that these combined tools will help cell biologists obtain large amounts of spatial and temporal information about pathways in living cells. Included in the proposal are programs for making the fluorescent probe and imaging technologies robust so that they can be provided to a wide range of cell biologists in academic research, pharmaceutical drug discovery, and biotechnology and to disseminate these technologies and provide training. PERFORMANCE

我们建议建立一个全国可见且响应迅速的中心，专注于荧光探针和成像 用于实时、高分辨率地研究活细胞中的通路和网络的技术。这 拟议的中心将通过结合两个现有的经验和基础设施来组建 中心位于匹兹堡。该中心的研究部分将创建一个强大的细胞内工具箱 荧光标记和生物传感器可用于研究许多（如果不是全部）通路中的蛋白质 活细胞网络。强荧光探针开发计划融合了遗传学、蛋白质 结构、核酸结构和荧光染料化学。我们建议生成的探针将是 可遗传表达，因此外源大分子不必运输到活细胞中 有待研究。 除了 技术开发核心。这些项目位于匹兹堡大学卡内基梅隆大学 斯坦福和伯克利。他们的职责是协助新中心确定最佳技术 用于开发并提供有关技术实用性的重要反馈。 成像和信息学技术与新的荧光探针技术相结合， 这些组合工具将帮助细胞生物学家获得大量的空间和时间信息 活细胞中的通路。该提案中包括制作荧光探针和成像的程序 技术强大，以便可以将其提供给广泛的细胞生物学家进行学术研究， 药物发现和生物技术，并传播这些技术并提供 训练。 表现