Flourescent Probes & Imaging - Networks & Pathways(RMI)

荧光探针

• 批准号: 7689390
• 负责人: Alan S. Waggoner
• 金额: $ 285.29万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7689390
• 关键词: Flourescent; Probes; Imaging; Networks; Pathways

We propose to form a nationally visible and responsive center focused on fluorescent probe and imaging technologies for investigating pathways and networks in real time and at high resolution in living cells. The proposed Center will be formed by combining the experience and infrastructure of two already existing Centers in Pittsburgh. The research component of the Center will create a powerful toolbox of intracellular fluorescent labels and biosensors that can be used to study many, if not all, the proteins in pathways and networks of living cells. The strong fluorescent probe development program blends genetics, protein structure, nucleic acid structure and fluorescent dye chemistry. The probes we propose to generate will be genetically expressible so that exogenous macromolecules will not have to be transported into the living cells to be studied. Four superb "Driving Biology Projects" are to be part of the overall Center program in addition to the Technology Development Core. These projects are located at the University of Pittsburgh, Carnegie Mellon, Stanford and Berkeley. Their responsibility is to assist the new center in identifying the optimal technologies for development and for providing crucial feedback regarding the utility of the technologies. Imaging and informatics technologies are integrated with the new fluorescent probe technologies so that these combined tools will help cell biologists obtain large amounts of spatial and temporal information about pathways in living cells. Included in the proposal are programs for making the fluorescent probe and imaging technologies robust so that they can be provided to a wide range of cell biologists in academic research, pharmaceutical drug discovery, and biotechnology and to disseminate these technologies and provide training. PERFORMANCE

我们建议成立一个全国可见的和响应中心，专注于荧光探针和成像 在活细胞中以真实的时间和高分辨率研究途径和网络的技术。的 拟议中的中心将结合现有的两个中心的经验和基础设施， 匹兹堡的中心。该中心的研究部分将创建一个强大的细胞内 荧光标记和生物传感器，可用于研究许多，如果不是全部，蛋白质的途径， 活细胞网络。强荧光探针开发计划融合遗传学、蛋白质 结构、核酸结构和荧光染料化学。我们建议生成的探测器将是 可遗传表达，因此外源大分子不必被转运到活细胞中 以供研究。 四个极好的“驾驶生物学项目”是整个中心计划的一部分，除了 技术开发核心。这些项目位于匹兹堡大学卡内基梅隆大学， 斯坦福大学和伯克利。他们的职责是协助新中心确定最佳技术 并就技术的实用性提供关键反馈。 成像和信息学技术与新的荧光探针技术相结合， 这些组合工具将帮助细胞生物学家获得大量的空间和时间信息， 活细胞中的通路。该提案中包括制作荧光探针和成像的计划 强大的技术，使他们可以提供给广泛的细胞生物学家在学术研究， 药物发现和生物技术，并传播这些技术， 训练 性能