Hyperspectral Imaging of Oxygen Saturation in the ONH

ONH 中氧饱和度的高光谱成像

• 批准号: 6671433
• 负责人: Bahram Khoobehi
• 金额: $ 13.22万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6671433
• 关键词: Macaca fascicularis; bioimaging /biomedical imaging; diagnosis design /evaluation; eye circulation; eye circulation disorder; eye disorder diagnosis; eye fundus photography; glaucoma; intraocular pressure; optic nerve; oximetry; oxygen consumption; oxygen transport; retina

DESCRIPTION (provided by applicant): The development of a non-invasive means of measuring oxygen saturation in the fundus of the human eye would be useful in the diagnosis and monitoring of numerous disorders, including diabetic retinopathy, arterial venous occlusion disease, and glaucoma. In these studies, a practical system to evaluate oxygen saturation in the retina and optic nerve head using a recent innovation, hyperspectral imaging, will be developed. The hyperspectral technique measures spectral changes within the visible and infrared spectra and provides information on the molecular state of hemoglobin. The hyperspectral imaging device will allow measurement - non-invasively and in real time - of reduction and/or elevation in tissue oxygenation. The distinct optical signature of biological materials such as oxy-hemoglobin and deoxy-hemoglobin as a function of their reflectance spectra will enable determination of their relative concentrations. In recent years, reflectance oximetry has been developed for the non-invasive measurement of oxygen saturation changes in the vessels of the fundus using double, triple, and multiple wavelength reflectance imaging. The hyperspectral reflectance oximetry that will be employed in these studies will permit the first non-invasive measurement for oxygen saturation in the optic nerve head tissue, and the hyperpectral data to be collected will intrinsically include all of the multiple wavelength spectra obtained in earlier approaches. The new system will be tested in two specific aims: 1) hyperspectral imaging will be used to non-invasively evaluate the stimulus-response relationship between perturbations in intraocular pressure (lOP) (10-50 mm Hg) and oxygen saturation in optic nerve head tissues and in retinal artery/vein pairs for a graded series of hypoxic states, and 2) the same studies will be performed in eyes with early stage experimental glaucoma. With this new approach, it will be possible to determine how acute changes in lOP alone or in combination with chronic lOP elevation (glaucoma) affect the three distinct microcirculations of the optic nerve head (surface nerve fiber layer; prelaminar region; lamina cribrosa) independently and/or collectively. The proposed studies are motivated by the potential for clinical application of this innovative technology in the early diagnosis of and monitoring of therapy for ocular vascular diseases in which the associated hypoxia may eventually lead to loss of vision.

描述（由申请人提供）：开发一种测量人眼眼底氧饱和度的非侵入性方法，可用于诊断和监测多种疾病，包括糖尿病视网膜病变、动脉静脉闭塞疾病和青光眼。 在这些研究中，将开发一种实用的系统，使用最近的创新，高光谱成像来评估视网膜和视神经乳头中的氧饱和度。 高光谱技术测量可见光和红外光谱内的光谱变化，并提供有关血红蛋白分子状态的信息。高光谱成像设备将允许非侵入性地并且在真实的时间内测量组织氧合的降低和/或升高。 生物材料如氧合血红蛋白和脱氧血红蛋白的不同光学特征作为其反射光谱的函数将使得能够确定其相对浓度。 近年来，已经开发了反射式血氧测定法，用于使用双波长、三波长和多波长反射成像来非侵入性地测量眼底血管中的氧饱和度变化。 将在这些研究中采用的高光谱反射血氧测定法将允许第一次非侵入性测量视神经头组织中的氧饱和度，并且要收集的高光谱数据将本质上包括在早期方法中获得的所有多波长光谱。 新系统将在两个具体目标方面进行测试：1）高光谱成像将用于无创评估眼内压（IOP）扰动之间的刺激-反应关系（10-50 mm Hg）和氧饱和度，用于分级系列缺氧状态，和2）将在具有早期实验性青光眼的眼睛中进行相同的研究。 通过这种新方法，将有可能确定单独或与慢性IOP升高（青光眼）组合的IOP的急性变化如何独立地和/或共同地影响视神经乳头的三种不同的微循环（表面神经纤维层;前板区;筛板）。 提出的研究的动机是这种创新技术在眼血管疾病的早期诊断和治疗监测中的临床应用潜力，其中相关的缺氧可能最终导致视力丧失。