RETINAL AND CHOROIDAL BLOOD FLOW IMAGING
视网膜和脉络膜血流成像
基本信息
- 批准号:6054926
- 负责人:
- 金额:$ 21.3万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2000
- 资助国家:美国
- 起止时间:2000-04-01 至 2003-03-31
- 项目状态:已结题
- 来源:
- 关键词:Macaca mulatta cell migration choroid uvea diabetes mellitus diagnosis design /evaluation erythrocytes eye circulation eye disorder diagnosis fluorescent dye /probe hemodynamics intraocular pressure laboratory rat leukocytes macular drusen microcirculation optic nerve retina circulation statistics /biometry ultrasound blood flow measurement
项目摘要
DESCRIPTION (Author supplied): This proposed study focuses on fluorescent cell
imaging (FCI) in which erythrocyte membranes or leukocyte nuclei from one
animal are stained with different fluorescent dyes of different wavelengths,
injected systemically in the same animal, excited transcorneally, and tracked
and analyzed to quantify blood flow. After imaging fluorescent cells in the
blood vessels of the posterior pole of the eye, video frames are analyzed to
infer maximum and average blood cell velocities, and relative and absolute
volumetric blood flow using an automated data extraction system. The diameters
of large ocular blood vessels are measured and mapped. Cell velocities are
spectrally analyzed to determine pulsatility. The five objectives of the study
are: 1) to establish FCI safety and potential use in humans by performing
toxicity tests in rats and monkeys; 2) to develop a statistical framework for
comparing parameter maps; 3) to validate FCI by evaluating measurements made in
vitro of stained erythrocytes in capillary tubes and in vivo by comparing FCI
measurements of velocities and flow rates in the monkey eye to measurements
obtained using laser Doppler methods; 4) to compare erythrocyte and leukocyte
blood flow velocities in the microcirculation; and 5) to assess the clinical
utility of FCI. The fifth goal has four parts. First, FCI will be used to
compare blood parameters in a rat model before and after inducing symptoms of
diabetes using streptozocin, and in a researcher-blind study, an attempt will
be made to use FCI parameter maps to diagnose diabetes. Second, quantification
and mapping of blood speeds, pulsatility, and flow rates in the
microcirculation of the macula and optic nerve head of a normal monkey will be
performed using FCI. Third, those hemodynamic parameters will be mapped in the
macular microcirculation of the monkey choroid using FCI. Finally, changes in
hemodynamic parameter maps of the monkey eye in response to an acute increase
in intraocular pressure will be quantified. With FCI, changes in blood flow
rates in diabetic retinopathy and glaucoma might be determined, as well as the
effect of consumption of vasoactive drugs.
描述(作者提供):这项拟议研究的重点是荧光细胞
在FCI中,来自一个细胞的红细胞膜或白细胞核
动物被不同波长的不同荧光染料染色,
在同一只动物体内全身注射,经角膜兴奋,
并进行分析以量化血流。在对荧光细胞进行成像后,
眼睛后极的血管,分析视频帧,
推断最大和平均血细胞速度,以及相对和绝对速度
使用自动数据提取系统测量血流量。直径
测量并绘制了大的眼部血管。细胞速度是
光谱分析以确定脉动性。研究的五个目标
是:1)通过执行以下操作来确定FCI在人类中的安全性和潜在用途:
在大鼠和猴子中进行毒性试验; 2)制定统计框架,
比较参数图; 3)通过评估在
通过比较FCI,体外毛细管和体内染色红细胞
猴眼中的速度和流速测量值与
采用激光多普勒方法获得; 4)比较红细胞和白细胞
微循环中的血流速度;和5)评估临床
FCI的效用。第五个目标有四个部分。首先,FCI将用于
在诱导症状之前和之后比较大鼠模型中的血液参数,
在一项研究者盲法研究中,
使用FCI参数图来诊断糖尿病。第二,量化
以及绘制血液速度、脉动性和流速,
正常猴子的黄斑和视神经头的微循环将被
使用FCI。第三,这些血流动力学参数将在
使用FCI的猴脉络膜黄斑微循环。最后,
猴眼的血液动力学参数图对急性增加的反应
眼内压将被量化。通过FCI,血流变化
糖尿病视网膜病变和青光眼的发病率可能会被确定,
消耗血管活性药物的影响。
项目成果
期刊论文数量(0)
专著数量(0)
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会议论文数量(0)
专利数量(0)
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Bahram Khoobehi其他文献
Bahram Khoobehi的其他文献
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{{ truncateString('Bahram Khoobehi', 18)}}的其他基金
Hyperspectral Imaging of Oxygen Saturation in the ONH
ONH 中氧饱和度的高光谱成像
- 批准号:
6928992 - 财政年份:2003
- 资助金额:
$ 21.3万 - 项目类别:
Hyperspectral Imaging of Oxygen Saturation in the ONH
ONH 中氧饱和度的高光谱成像
- 批准号:
6671433 - 财政年份:2003
- 资助金额:
$ 21.3万 - 项目类别:
Hyperspectral Imaging of Oxygen Saturation in the ONH
ONH 中氧饱和度的高光谱成像
- 批准号:
7234547 - 财政年份:2003
- 资助金额:
$ 21.3万 - 项目类别:
Hyperspectral Imaging of Oxygen Saturation in the ONH
ONH 中氧饱和度的高光谱成像
- 批准号:
6778221 - 财政年份:2003
- 资助金额:
$ 21.3万 - 项目类别:
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