RETINAL AND CHOROIDAL BLOOD FLOW IMAGING

描述（作者提供）：这项拟议研究的重点是荧光细胞 在FCI中，来自一个细胞的红细胞膜或白细胞核 动物被不同波长的不同荧光染料染色， 在同一只动物体内全身注射，经角膜兴奋， 并进行分析以量化血流。在对荧光细胞进行成像后， 眼睛后极的血管，分析视频帧， 推断最大和平均血细胞速度，以及相对和绝对速度 使用自动数据提取系统测量血流量。直径 测量并绘制了大的眼部血管。细胞速度是 光谱分析以确定脉动性。研究的五个目标 是：1）通过执行以下操作来确定FCI在人类中的安全性和潜在用途： 在大鼠和猴子中进行毒性试验; 2）制定统计框架， 比较参数图; 3）通过评估在 通过比较FCI，体外毛细管和体内染色红细胞 猴眼中的速度和流速测量值与 采用激光多普勒方法获得; 4）比较红细胞和白细胞 微循环中的血流速度;和5）评估临床 FCI的效用。第五个目标有四个部分。首先，FCI将用于 在诱导症状之前和之后比较大鼠模型中的血液参数， 在一项研究者盲法研究中， 使用FCI参数图来诊断糖尿病。第二，量化 以及绘制血液速度、脉动性和流速， 正常猴子的黄斑和视神经头的微循环将被 使用FCI。第三，这些血流动力学参数将在 使用FCI的猴脉络膜黄斑微循环。最后， 猴眼的血液动力学参数图对急性增加的反应 眼内压将被量化。对于FCI，血流变化 糖尿病视网膜病变和青光眼的发病率可能会被确定， 消耗血管活性药物的影响。

DESCRIPTION (Author supplied): This proposed study focuses on fluorescent cell imaging (FCI) in which erythrocyte membranes or leukocyte nuclei from one animal are stained with different fluorescent dyes of different wavelengths, injected systemically in the same animal, excited transcorneally, and tracked and analyzed to quantify blood flow. After imaging fluorescent cells in the blood vessels of the posterior pole of the eye, video frames are analyzed to infer maximum and average blood cell velocities, and relative and absolute volumetric blood flow using an automated data extraction system. The diameters of large ocular blood vessels are measured and mapped. Cell velocities are spectrally analyzed to determine pulsatility. The five objectives of the study are: 1) to establish FCI safety and potential use in humans by performing toxicity tests in rats and monkeys; 2) to develop a statistical framework for comparing parameter maps; 3) to validate FCI by evaluating measurements made in vitro of stained erythrocytes in capillary tubes and in vivo by comparing FCI measurements of velocities and flow rates in the monkey eye to measurements obtained using laser Doppler methods; 4) to compare erythrocyte and leukocyte blood flow velocities in the microcirculation; and 5) to assess the clinical utility of FCI. The fifth goal has four parts. First, FCI will be used to compare blood parameters in a rat model before and after inducing symptoms of diabetes using streptozocin, and in a researcher-blind study, an attempt will be made to use FCI parameter maps to diagnose diabetes. Second, quantification and mapping of blood speeds, pulsatility, and flow rates in the microcirculation of the macula and optic nerve head of a normal monkey will be performed using FCI. Third, those hemodynamic parameters will be mapped in the macular microcirculation of the monkey choroid using FCI. Finally, changes in hemodynamic parameter maps of the monkey eye in response to an acute increase in intraocular pressure will be quantified. With FCI, changes in blood flow rates in diabetic retinopathy and glaucoma might be determined, as well as the effect of consumption of vasoactive drugs.