RETINAL AND CHOROIDAL BLOOD FLOW IMAGING

视网膜和脉络膜血流成像

• 批准号: 6384854
• 负责人: Bahram Khoobehi
• 金额: $ 22.31万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6384854
• 关键词: Macaca mulatta; cell migration; choroid uvea; computer data analysis; diabetes mellitus; diagnosis design /evaluation; erythrocytes; eye circulation; eye disorder diagnosis; fluorescent dye /probe; hemodynamics; intraocular pressure; laboratory rat; leukocytes; macular drusen; microcirculation; optic nerve; retina circulation; statistics /biometry; ultrasound blood flow measurement

DESCRIPTION (Author supplied): This proposed study focuses on fluorescent cell imaging (FCI) in which erythrocyte membranes or leukocyte nuclei from one animal are stained with different fluorescent dyes of different wavelengths, injected systemically in the same animal, excited transcorneally, and tracked and analyzed to quantify blood flow. After imaging fluorescent cells in the blood vessels of the posterior pole of the eye, video frames are analyzed to infer maximum and average blood cell velocities, and relative and absolute volumetric blood flow using an automated data extraction system. The diameters of large ocular blood vessels are measured and mapped. Cell velocities are spectrally analyzed to determine pulsatility. The five objectives of the study are: 1) to establish FCI safety and potential use in humans by performing toxicity tests in rats and monkeys; 2) to develop a statistical framework for comparing parameter maps; 3) to validate FCI by evaluating measurements made in vitro of stained erythrocytes in capillary tubes and in vivo by comparing FCI measurements of velocities and flow rates in the monkey eye to measurements obtained using laser Doppler methods; 4) to compare erythrocyte and leukocyte blood flow velocities in the microcirculation; and 5) to assess the clinical utility of FCI. The fifth goal has four parts. First, FCI will be used to compare blood parameters in a rat model before and after inducing symptoms of diabetes using streptozocin, and in a researcher-blind study, an attempt will be made to use FCI parameter maps to diagnose diabetes. Second, quantification and mapping of blood speeds, pulsatility, and flow rates in the microcirculation of the macula and optic nerve head of a normal monkey will be performed using FCI. Third, those hemodynamic parameters will be mapped in the macular microcirculation of the monkey choroid using FCI. Finally, changes in hemodynamic parameter maps of the monkey eye in response to an acute increase in intraocular pressure will be quantified. With FCI, changes in blood flow rates in diabetic retinopathy and glaucoma might be determined, as well as the effect of consumption of vasoactive drugs.

描述（作者提供）：本研究的重点是荧光细胞

项目成果

A novel approach for freeze-frame video determination of volumetric blood flow in the rat retina.

• DOI: 10.3928/1542-8877-20031101-16
• 发表时间: 2003-11
• 期刊: Ophthalmic surgery, lasers & imaging : the official journal of the International Society for Imaging in the Eye
• 作者: B. Khoobehi;G. Peyman;L. G. Carnahan;R. L. Hayes