RETINAL AND CHOROIDAL BLOOD FLOW IMAGING

描述(作者提供)：这项拟议的研究重点是荧光细胞 红细胞膜或白细胞核形成的一种成像技术 用不同波长的不同荧光染料对动物进行染色， 在同一动物体内系统注射，经角膜激发，并进行跟踪 并进行分析以量化血流量。在对细胞内的荧光细胞成像后 对眼后极血管、视频帧进行分析以 推断最大和平均血细胞速度，以及相对和绝对速度 使用自动数据提取系统测量血流量。它的直径 对眼大血管进行测量和测绘。细胞速度是 光谱分析以确定搏动性。研究的五个目标 是：1)通过执行以下步骤来确定FCI在人类中的安全性和潜在用途 对老鼠和猴子的毒性测试；2)建立一个统计框架 比较参数图；3)通过评估在以下方面的测量来验证FCI 毛细管染色红细胞体外与体内FCI的比较 猴眼速度和流速的测量与测量 用激光多普勒法获得；4)比较红细胞和白细胞 微循环中的血流速度；5)评估临床 FCI的效用。第五个目标由四个部分组成。首先，FCI将用于 大鼠模型诱导症状前后血液参数的比较 糖尿病使用链脲佐菌素，在一项研究人员盲目研究中，一项尝试将 使用FCI参数图来诊断糖尿病。第二，量化 并绘制了血流速度、脉动度和流速的地图 正常猴黄斑和视神经头的微循环 使用FCI执行。第三，这些血流动力学参数将被映射到 应用FCI技术观察猕猴脉络膜黄斑微循环。最后，变化 猴眼对急剧升高的血流动力学参数图 眼压的变化将被量化。对于FCI，血流的变化 糖尿病视网膜病变和青光眼的发病率可能会被确定，以及 使用血管活性药物的影响。

DESCRIPTION (Author supplied): This proposed study focuses on fluorescent cell imaging (FCI) in which erythrocyte membranes or leukocyte nuclei from one animal are stained with different fluorescent dyes of different wavelengths, injected systemically in the same animal, excited transcorneally, and tracked and analyzed to quantify blood flow. After imaging fluorescent cells in the blood vessels of the posterior pole of the eye, video frames are analyzed to infer maximum and average blood cell velocities, and relative and absolute volumetric blood flow using an automated data extraction system. The diameters of large ocular blood vessels are measured and mapped. Cell velocities are spectrally analyzed to determine pulsatility. The five objectives of the study are: 1) to establish FCI safety and potential use in humans by performing toxicity tests in rats and monkeys; 2) to develop a statistical framework for comparing parameter maps; 3) to validate FCI by evaluating measurements made in vitro of stained erythrocytes in capillary tubes and in vivo by comparing FCI measurements of velocities and flow rates in the monkey eye to measurements obtained using laser Doppler methods; 4) to compare erythrocyte and leukocyte blood flow velocities in the microcirculation; and 5) to assess the clinical utility of FCI. The fifth goal has four parts. First, FCI will be used to compare blood parameters in a rat model before and after inducing symptoms of diabetes using streptozocin, and in a researcher-blind study, an attempt will be made to use FCI parameter maps to diagnose diabetes. Second, quantification and mapping of blood speeds, pulsatility, and flow rates in the microcirculation of the macula and optic nerve head of a normal monkey will be performed using FCI. Third, those hemodynamic parameters will be mapped in the macular microcirculation of the monkey choroid using FCI. Finally, changes in hemodynamic parameter maps of the monkey eye in response to an acute increase in intraocular pressure will be quantified. With FCI, changes in blood flow rates in diabetic retinopathy and glaucoma might be determined, as well as the effect of consumption of vasoactive drugs.