权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Biocompatible PEG-Functionalized Methacrylates

生物相容性 PEG 功能化甲基丙烯酸酯

基本信息

批准号：
6645425
负责人：
WILLIAM E COLLINS
金额：
$ 13.84万
依托单位：
HOWARD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-08-01 至 2005-05-31
项目状态：
已结题

项目摘要

DESCRIPTION: (Applicant's Abstract) This project investigates the potential of functionalizing poly methyl methcrylate (PMMA) with poly (ethylene glycol) (PEG) to inhibit epithelial cell in growth and inflammatory reactions to the prospective intraocular lens. PMMA displays strong posterior attachment, but some in-growth. Trace functionalization with PEG will further inhibit undesired biological responses without disturbing the strong posterior attachment of PMMA. A suitable in vitro experimental model will measure these responses at low cost to determine if in vivo pursuit is worthwhile. This model uses the human corneal epithelial cell line HCE- I as an epithelial cell model. HCE-1 cells will be cultured, passaged and placed onto the different biomaterials. Adherent cells will be visualized and counted using inverted microscopy and computerized image analysis. Adhesion will be based on the spread area/cell and fraction of biomaterial surface occupied by adherent cells. HCE-1 cells will be incubated on bare and protein-preadsorbed PEG-functionalized PMMA to reveal the role of proteins in promoting cell adhesion. The proteins selected are human fibronectin, an adhesive RGD protein, and serum albumin, a nonadhesive protein. Human fibronectin will be isolated from citrated whole human blood and characterized, including its biological activity. Protein adsorption will be quantified radiolabelling proteins with 125, using the chloramine-T method. This project will synthesize and characterize PMMA pendantly functionalized with poly(ethylene glycol), including ESCA and contact angle goniometry. Goniometry of protein-preadsorbed polymers will be endeavored. Macrophage adhesion will be quantified to appraise possible inflammatory reactions that these biomaterials can elicit. Macrophages will isolated from whole human blood by centrifugation on a Ficoll-Hypaque gradient. Macrophage chemotaxis and phagocytosis will be assessed. The morphology of adherent macrophages and HCE- I cells will also be examined with scanning electron microscopy.

描述：（申请人的摘要）该项目调查了用聚乙二醇对聚甲基丙烯酸甲酯 (PMMA) 进行功能化 (PEG) 抑制上皮细胞的生长和炎症反应前瞻性人工晶状体。 PMMA 显示出很强的后附着力，但是一些内向增长。 PEG 的微量功能化将进一步抑制不良反应生物反应而不干扰牢固的后部附着聚甲基丙烯酸甲酯。合适的体外实验模型将以低成本测量这些反应以确定体内追求是否值得。该模型使用人类角膜上皮细胞系HCE-I作为上皮细胞模型。 HCE-1细胞将被培养、传代并放置在不同的生物材料上。依附者使用倒置显微镜和计算机对细胞进行可视化和计数图像分析。粘附力将基于扩散面积/细胞和分数生物材料表面被贴壁细胞占据。 HCE-1细胞将被孵化在裸露和蛋白质预吸附的 PEG 功能化 PMMA 上揭示了促进细胞粘附的蛋白质。选择的蛋白质是人类的纤连蛋白（一种粘附性 RGD 蛋白）和血清白蛋白（一种非粘附性蛋白）。人纤连蛋白将从柠檬酸化的人全血中分离出来，特征，包括其生物活性。蛋白质吸附将使用氯胺-T 方法对 125 的放射性标记蛋白进行定量。该项目将合成并表征 PMMA 悬垂功能化聚乙二醇，包括 ESCA 和接触角测角仪。将努力对蛋白质预吸附聚合物进行测角。巨噬细胞粘附将被量化以评估可能的炎症反应这些生物材料可以引发。将从人类全血中分离出巨噬细胞通过 Ficoll-Hypaque 梯度离心。巨噬细胞趋化性和将评估吞噬作用。贴壁巨噬细胞和 HCE 的形态 I 细胞也将通过扫描电子显微镜进行检查。