Detection Of Toxigenic Clostridium Difficile In Stool By

艰难梭菌相关性疾病（CDAD）是接受抗生素或抗生素治疗的住院患者的主要问题。目前用于诊断CDAD的实验室方法包括用于检测由生物体或细胞相关抗原产生的毒素的培养和测定。在文献中已经报道了用于诊断CDAD的PCR检测，但是这些报道在范围上是有限的，并且所使用的许多引物对是低效的或者扩增C. difficile基因组我们筛选了5对特异性引物，分别针对两种毒素基因进行了PCR扩增。很难我们正在选择两个引物对，这两个引物对在扩增其目标方面最有效。然后，我们将使用Light Cycler开发实时PCR，并使用该检测方法评估PCR在粪便标本诊断CDAD中的实用性。

Clostridium difficile-associated disease (CDAD) is a major problem for hospitalized patients recieving antibiotics or antineoplastic agents. Current labotatory methods for diagnosing CDAD include culture and assays for detection of the toxins produced by the organism or a cell-associated antigen. PCR assays for the diagnosis of CDAD have been reported in the liturature, but these reports are limited in scope and many of the primer pairs used are inefficient or amplify inappropriate portions of the C. difficile genome. We have selected 5 primer pairs specific for the two toxin genes of C. difficile. We are in the process of selecting the two primer pairs that are the most efficient in amplifying their targets. We will then develop a realtime PCR using the Light Cycler and will use this assay to evaluate the utility of PCR for the diagnosis of CDAD using stool specimens.