Performance of Rapid Tests for Detection of Avian Influe

禽流感快速检测的性能

• 批准号: 7332561
• 负责人: DANIEL P. FEDORKO
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7332561
• 关键词: Performance; Rapid; Tests; Detection; Avian

Our initial investigation revealed that two rapid antigen capture enzyme immunoassays (EIA) did not detect wild type avian influenza virus type H5N1 [A/Vietnam/1203/2004(H5N1)] at a concentration of 50,000 TCID50. We challenged the rapid EIAs with a 10 fold dilution series of wild type viruses from 10 to 1,000,000 TCID50 and found the EIAs could detect A/Vietnam/1203/2004(H5N1) and A/Indonesia/05/2005(H5N1) at 1,000,000 and 100,000 TCID50 respectively, but neither assay detected A/Hong Kong/491/1997(H5N1) at any dilution. These data suggest that these assays require high virus concentrations, such as those found in nasopharyngeal washes from symptomatic patients early in their infections, to detect avian influenza virus type H5N1.

我们的初步调查显示，两种快速抗原捕获酶免疫测定法（EIA）在50，000 TCID 50浓度下未检测到野生型H5 N1型禽流感病毒[A/Vietnam/1203/2004（H5 N1）]。我们用野生型病毒的10倍稀释系列从10到1，000，000 TCID 50挑战快速EIA，发现EIA可以分别检测1，000，000和100，000 TCID 50的A/Vietnam/1203/2004（H5 N1）和A/Indonesia/05/2005（H5 N1），但在任何稀释度下，两种检测方法均未检测到A/Hong Kong/491/1997（H5 N1）。这些数据表明，这些检测需要高病毒浓度，如在感染早期症状患者的鼻咽冲洗液中发现的病毒浓度，以检测H5 N1型禽流感病毒。