Performance of Rapid Tests for Detection of Avian Influe

在甲型禽流感病毒亚型中，H5N1和H9N2病毒有可能引起流感大流行，因为它们在亚洲的禽类中广泛流行，并已证明能够感染人类。我们试图确定是否快速检测常规用于临床微生物学实验室，使用A549和RhMK细胞和两个商业抗原捕获酶免疫测定（EIA）的壳瓶测定，可以检测野生型（wt）和冷适应（ca）禽流感A H5N1和H9N2病毒。在20和20，000 TCID50浓度下，孵育1、2和5天后，在A549和RhMK细胞中同样良好地检测到H5N1 ca病毒。在两种细胞系中也检测到2 × 104 TCID50的H5N1 wt病毒，但在20 TCID50时，仅在A549细胞中第5天检测到wt病毒，在RhMK细胞中第2天和第5天检测到wt病毒。孵育1、2和5天后，在两种浓度下，在两种细胞系中同样良好地检测到H9N2 wt病毒。在20，000 TCID50时，在两种细胞系中也检测到H9N ca病毒，但在20 TCID50时，仅在A549细胞中的第5天以及RhMK细胞中的第2天和第5天检测到ca病毒。我们发现，对于商业EIA，H9N2 wt和ca病毒在50，000 TCID50下通过两种测定法检测到。在两种试验中均在50，000 TCID50下检出H5N1 ca病毒，但在该浓度下，两种试验均未检出wt病毒。

Among the avian influenza A virus subtypes, the H5N1 and H9N2 viruses have the potential to cause an influenza pandemic because they are widely prevalent in avian species in Asia and have demonstrated the ability to infect humans. We sought to determine if rapid assays routinely used in clinical microbiology laboratories, the shell vial assay using A549 and RhMK cells and two commercial antigen capture enzyme immunoassays (EIA), could detect wild type (wt) and cold adapted (ca) avian influenza A H5N1 and H9N2 viruses. At concentrations of 20 and 20,000 TCID50 H5N1 ca virus was detected after 1, 2, and 5 days of incubation equally well in both A549 and RhMK cells. The H5N1 wt virus at 2 x 104 TCID50 was also detected in both cell lines, but at 20 TCID50 the wt virus was only detected at 5 days in A549 cells and at 2 and 5 days in RhMK cells. The H9N2 wt virus was detected at both concentrations after 1,2, and 5 days of incubation equally well in both cell lines. The H9N ca virus was also detected in both cell lines at 20,000 TCID50, but at 20 TCID50 the ca virus was only detected at 5 days in A549 cells and at 2 and 5 days in RhMK cells. We found for the commercial EIAs H9N2 wt and ca viruses were detected by both assays at 50,000 TCID50. The H5N1 ca virus was detected at 50,000 TCID50 in both assays, but the wt virus was not detected in either assay at this concentration.