Performance of Rapid Tests for Detection of Avian Influe

在甲型禽流感病毒亚型中，H5N1和H9N2病毒有可能引起流感大流行，因为它们在亚洲的禽类中广泛流行，并已证明具有感染人类的能力。我们试图确定临床微生物实验室常规使用的快速检测方法，使用A549和RhMK细胞的壳瓶法和两种商业抗原捕获酶免疫测定法（EIA）是否可以检测野生型（wt）和冷适应型（ca）禽流感H5N1和H9N2病毒。在浓度为20和20,000 TCID50的情况下，A549和RhMK细胞在孵育1、2和5天后均检测到H5N1 ca病毒。在2 × 104 TCID50条件下，在两种细胞系中也检测到H5N1 wt病毒，但在20 TCID50条件下，在A549细胞和RhMK细胞中仅在5天检测到wt病毒。在孵育1、2和5天后，两种浓度的H9N2 wt病毒在两种细胞系中均被检测到。在2万TCID50条件下，在两种细胞系中均检测到H9N ca病毒，但在20 TCID50条件下，仅在A549细胞和RhMK细胞中5天检测到ca病毒。我们发现，在5万TCID50条件下，两种方法都能检测到H9N2 wt和ca病毒。在5万TCID50浓度下，两项检测均检测到H5N1型ca病毒，但在该浓度下，两项检测均未检测到wt病毒。

Among the avian influenza A virus subtypes, the H5N1 and H9N2 viruses have the potential to cause an influenza pandemic because they are widely prevalent in avian species in Asia and have demonstrated the ability to infect humans. We sought to determine if rapid assays routinely used in clinical microbiology laboratories, the shell vial assay using A549 and RhMK cells and two commercial antigen capture enzyme immunoassays (EIA), could detect wild type (wt) and cold adapted (ca) avian influenza A H5N1 and H9N2 viruses. At concentrations of 20 and 20,000 TCID50 H5N1 ca virus was detected after 1, 2, and 5 days of incubation equally well in both A549 and RhMK cells. The H5N1 wt virus at 2 x 104 TCID50 was also detected in both cell lines, but at 20 TCID50 the wt virus was only detected at 5 days in A549 cells and at 2 and 5 days in RhMK cells. The H9N2 wt virus was detected at both concentrations after 1,2, and 5 days of incubation equally well in both cell lines. The H9N ca virus was also detected in both cell lines at 20,000 TCID50, but at 20 TCID50 the ca virus was only detected at 5 days in A549 cells and at 2 and 5 days in RhMK cells. We found for the commercial EIAs H9N2 wt and ca viruses were detected by both assays at 50,000 TCID50. The H5N1 ca virus was detected at 50,000 TCID50 in both assays, but the wt virus was not detected in either assay at this concentration.