Transfer of Organelle Genes to the Nucleus

将细胞器基因转移到细胞核

• 批准号: 6724896
• 负责人: JEFFREY D PALMER
• 金额: $ 38.91万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6724896
• 关键词: Arabidopsis; DNA directed RNA polymerase; angiosperms; biochemical evolution; cell nucleus; cytochrome oxidase; cytogenetics; developmental genetics; gene duplication; gene expression; gene induction /repression; gene mutation; genetic transcription; genome; introns; microarray technology; mitochondria; molecular cloning; nucleic acid hybridization; nucleic acid sequence; organelles; plant genetics; plant morphology; polymerase chain reaction; protein structure function

DESCRIPTION (Applicant's Abstract) The study of organelle genes is essential to our understanding of eukaryotic cellular and molecular biology. The proper functioning of organelles in eukaryotic cells involves a precise cooperation between nuclear and organellar genetic systems. Defects in this functioning have direct consequences for human health, as is evident from the intense interest in mitochondrially-inherited diseases. The primary goal of this proposal is to address a fundamental problem in the genetic coevolution of the eukaryotic cell: How are organelle genes functionally transferred to the nucleus during evolution, and what are the tempo, pattern, and consequences of these genetic relocations? All of this work is carried out in plants, which for several reasons should be viewed as a model to study functional gene transfer from organelles to nucleus. We propose to: 1) use DNA hybridization arrays to survey 20,000 species of plants in order to elucidate the fine-scale tempo and pattern of loss (and gain) of mitochondrial genes and introns, and to use these findings as the starting point for many of the more in-depth studies outlined below; 2) test, for several mitochondrial genes, the hypothesis that their many separate losses reflect remarkably high rates of functional gene transfer to the nucleus, and in so doing, to learn more about the mechanisms of functional gene transfer; 3) examine a few cases of gene transfer in-depth in order to characterize intermediate stages of transcompartmental duplication, to determine their persistence and the dynamics of mitochondrial vs. nuclear gene loss and silencing, and to investigate whether transcompartmental duplications are ever fixed; 4) identify plants with highly elevated rates of ongoing gene transfer and test the hypothesis that this is caused by highly elevated rates of reverse transcription; 5) determine whether mitochondrial genomes ever reacquire genes lost through transfer to the nucleus, and if so, whether this occurs by direct return from the nucleus of the same organism, or by lateral transfer from an unrelated organism; and. Our secondary goal is to determine the origin and pattern of transmission of an extraordinarily invasive genetic element - a homing group I intron that we showed has invaded mitochondrial coxi genes over 1,000 times through a massive wave of lateral transfers during recent angiosperm evolution.

（申请人摘要）细胞器基因的研究是

项目成果

Lack of a functional plastid tRNA(Cys) gene is associated with loss of photosynthesis in a lineage of parasitic plants.

功能性质体 tRNA(Cys) 基因的缺乏与寄生植物谱系中光合作用的丧失有关。

• DOI: 10.1007/bf00334780
• 发表时间: 1991
• 期刊: Current genetics
• 影响因子: 2.5
• 作者: Taylor,GW;Wolfe,KH;Morden,CW;dePamphilis,CW;Palmer,JD
• 通讯作者: Palmer,JD

Isolation and characterization of rad51 orthologs from Coprinus cinereus and Lycopersicon esculentum, and phylogenetic analysis of eukaryotic recA homologs.

灰盖鬼伞和番茄中 rad51 直向同源物的分离和表征，以及真核 reCA 同源物的系统发育分析。

• DOI: 10.1007/s002940050189
• 发表时间: 1997
• 期刊: Current genetics
• 影响因子: 2.5
• 作者: Stassen,NY;LogsdonJr,JM;Vora,GJ;Offenberg,HH;Palmer,JD;Zolan,ME
• 通讯作者: Zolan,ME

Long branch attraction, taxon sampling, and the earliest angiosperms: Amborella or monocots?

• DOI: 10.1186/1471-2148-4-35
• 发表时间: 2004-09-28
• 期刊: BMC evolutionary biology
• 影响因子: 3.4
• 作者: Stefanović S;Rice DW;Palmer JD
• 通讯作者: Palmer JD

Contrasting modes and tempos of genome evolution in land plant organelles.

陆地植物细胞器基因组进化的对比模式和节奏。

• DOI: 10.1016/0168-9525(90)90125-p
• 发表时间: 1990
• 期刊: Trends in genetics : TIG
• 作者: Palmer,JD

Physical and gene organization of mitochondrial DNA in fertile and male sterile sunflower. CMS-associated alterations in structure and transcription of the atpA gene.

可育和雄性不育向日葵线粒体 DNA 的物理和基因组织。

• DOI: 10.1093/nar/16.9.3787
• 发表时间: 1988
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Siculella,L;Palmer,JD
• 通讯作者: Palmer,JD