TRANSFER OF ORGANELLE GENES TO THE NUCLEUS
细胞器基因转移至细胞核
基本信息
- 批准号:2177727
- 负责人:
- 金额:$ 27.15万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1989
- 资助国家:美国
- 起止时间:1989-09-01 至 1996-11-30
- 项目状态:已结题
- 来源:
- 关键词:Arabidopsis Chlorophyta DNA directed RNA polymerase alternatives to animals in research angiosperms biochemical evolution cell nucleus chloroplasts cytogenetics developmental genetics eukaryote gene expression gene mutation genetic transcription messenger RNA molecular cloning northern blottings nucleic acid hybridization nucleic acid probes nucleic acid sequence organelles plant genetics polymerase chain reaction protein structure function southern blotting spliceosomes
项目摘要
The study of organelle genes and genomes is essential to our understand-
ing of eukaryotic cellular and molecular biology. The proper functioning
of organelles in all eukaryotic cells involves a precise cooperation
between nuclear and organelle genetic systems. Defects in this
functioning have direct consequences for human health, as is evident from
the recent explosion of reports on mitochondrially-inherited diseases.
This proposal addresses the fundamental problem in the genetic coevolut-
ion of the eukaryotic cell: How are organellar genes functionally
transferred to the nucleus and what are the consequences of these genetic
relocations?
First, several cases of recent gene transfer will be explored in order
to 1) gain insights into the timing and process of functional activation
of a transferred organellar gene within the nucleus; 2) examine the
prevalence of RNA intermediates and reverse transcription in the physical
act of sequence migration between cellular compartments; and 3) test the
hypothesis that spliceosomal-dependent nuclear pre-MRNA introns evolved
from self-splicing group II introns.
Second, a more ancient transfer to the nucleus, of the chloroplast gene
tufA, will be examined to test two hypotheses relating to gene function:
1) that this gene transfer allowed, in the green alga Coleochaete, a
novel situation of cross-compartmental gene amplification, with
functional divergence among the gene copies, and 2) that this same
transfer, in the land plant Arabidopsis, resulted in the nearly opposite
(and also novel) situation, whereby one nuclear gene supplies both the
chloroplast and mitochondrion with the same protein.
Finally, we will continue to study, using a variety of molecular
evolutionary approaches, the dramatically accelerated molecular evolution
of the chloroplast-encoded RNA polymerase in geranium.
细胞器基因和基因组的研究对我们理解-
真核细胞和分子生物学。 正常运作
所有真核细胞中的细胞器都需要精确的协作
细胞核遗传系统与细胞器遗传系统之间的联系。 网站的缺陷
功能对人类健康有直接影响,这一点从以下几点可以看出:
最近关于遗传性疾病的大量报道。
这一建议解决了遗传共同进化中的基本问题-
真核细胞的离子:细胞器基因如何发挥功能
转移到细胞核中,这些基因的结果是什么?
搬迁
首先,我们将探讨几个最近的基因转移案例,
1)深入了解功能激活的时间和过程
细胞核内转移的细胞器基因; 2)检查
RNA中间体和逆转录在物理中的流行
细胞区室之间的序列迁移行为;和3)测试
剪接体依赖核前mRNA内含子进化假说
来自自我剪接的第二组内含子
第二种是更古老的叶绿体基因向细胞核的转移
tufA将被检查以检验与基因功能相关的两个假设:
1)这种基因转移允许在绿色藻类鞘毛藻中产生
跨室基因扩增的新情况,
基因拷贝之间的功能差异,2)这同一个
转移,在陆地植物拟南芥中,导致了几乎相反的结果。
(and也是新的)情况,即一个核基因提供两个
叶绿体和叶绿体含有相同的蛋白质。
最后,我们将继续研究,利用各种分子
进化的方法,急剧加速的分子进化
叶绿体编码的RNA聚合酶。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JEFFREY D PALMER其他文献
JEFFREY D PALMER的其他文献
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{{ truncateString('JEFFREY D PALMER', 18)}}的其他基金
Genomic studies of chimeric mitochondria in cybrids from Solanaceae
茄科杂种嵌合线粒体的基因组研究
- 批准号:
7911843 - 财政年份:2009
- 资助金额:
$ 27.15万 - 项目类别:
Genomic studies of chimeric mitochondria in cybrids from Solanaceae
茄科杂种嵌合线粒体的基因组研究
- 批准号:
7694547 - 财政年份:2009
- 资助金额:
$ 27.15万 - 项目类别:
Genomic studies of chimeric mitochondria in cybrids from Solanaceae
茄科杂种嵌合线粒体的基因组研究
- 批准号:
8076901 - 财政年份:2009
- 资助金额:
$ 27.15万 - 项目类别:
Horizontal Gene Transfer in Multicellular Eukaryotes
多细胞真核生物中的水平基因转移
- 批准号:
6875514 - 财政年份:2005
- 资助金额:
$ 27.15万 - 项目类别:
Horizontal Gene Transfer in Multicellular Eukaryotes
多细胞真核生物中的水平基因转移
- 批准号:
7009271 - 财政年份:2005
- 资助金额:
$ 27.15万 - 项目类别:
Horizontal Gene Transfer in Multicellular Eukaryotes
多细胞真核生物中的水平基因转移
- 批准号:
7174292 - 财政年份:2005
- 资助金额:
$ 27.15万 - 项目类别:
Horizontal Gene Transfer in Multicellular Eukaryotes
多细胞真核生物中的水平基因转移
- 批准号:
7348367 - 财政年份:2005
- 资助金额:
$ 27.15万 - 项目类别:
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