BAC Library Production /Distribuion for 'Healthy people

BAC 图书馆为“健康人”制作/分发

• 批准号: 6946540
• 负责人: ROD A WING
• 金额: $ 2.5万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6946540
• 关键词: animal genetic material tag; artificial chromosomes; biomedical resource; biotechnology; cooperative study; genetic library; human genetic material tag; molecular biology information system; nucleic acid hybridization; technology /technique development; tissue resource /registry

DESCRIPTION: (provided by applicant) Cloning large DNA fragments using the bacterial artificial chromosome (BAC) library system has revolutionized and accelerated our ability to physically map, positionally clone and sequence whole genomes. Due to the difficulty in cloning large DNA fragments, only a handful of laboratories have been able to produce deep-coverage large insert libraries on a routine basis. This bottleneck has resulted in a limited number of BAC libraries that are available to the research community that can be used for gene discovery, survey sequencing and comparative genome studies. To increase the world?s BAC library construction capacity and reduce costs, the NIH has posted a request for applications (RFA.HG-Ol-002) entitled "BAC Library Production" which is the subject of this application. The specific aims of our thee-year proposal are: BAC Library Production: Develop BAC library construction capacity to construct 36 human genome size ( about3000 Mb) 10X genome equivalent BAC libraries. In year 1, we will produce 6 libraries at $0.50/clone, in year 2, 12 libraries will be produced at $0.375/clone and in year three 18 libraries will he produced at $0.25/clone. The initial average insert size will hover between 130 - 150 kb and we will strive to increase our average insert sizes to 170 - 200 kb in subsequent years. Libraries, filters and clones will be distributed through our CUGI BAC/EST Resource Center on a cost recovery basis (www.genome.clemson.edu). BAC Library Research and Development: We will systematically develop technologies to optimize and streamline BAC library construction procedures in order to reduce the cost of making libraries while maintaining quality and insert size. Also, technologies will be developed to routinely achieve insert sizes of 160kb or greater. In addition, a robust tool kit will be established to produce high-quality megabase-size DNA from animals. This will include the development of a "BIG DNA To-Go" kit to produce high-quality megabase-size DNA on location where the lab needing a library will provide the tissue(s) required to make the library. Bioinformatics Support: A laboratory information management/bar coding system will be developed to aid in tracking library production and distribution and a robust online BAC library ordering system and database will also be developed.

描述：（由申请人提供）使用 细菌人工染色体（BAC）文库系统已经发生了革命性变化， 加速了我们的物理映射，定位克隆和测序的能力 全基因组由于很难克隆大的DNA片段， 少数几个实验室已经能够生产深覆盖的大插入物 图书馆在日常生活中这一瓶颈导致了数量有限的 BAC的图书馆，可用于研究社区， 用于基因发现、调查测序和比较基因组研究。到 增加世界？的BAC库建设能力和降低成本， NIH已经发布了一个名为“BAC库"的申请请求（RFA.HG-Ol-002 生产”，这是本申请的主题。 我们三年计划的具体目标是： BAC图书馆生产：发展BAC图书馆建设能力， 36个人类基因组大小（约3000 Mb）的10倍基因组当量BAC文库。在 第一年，我们将以0.50美元/克隆的价格生产6个文库，第二年，生产12个文库 将以0.375美元/克隆的价格生产，第三年将有18个图书馆 以0.25美元/克隆生产。初始平均插入尺寸将徘徊在130 - 150 kb，我们将努力增加我们的平均插入大小到170 - 200 KB在接下来的几年里。库、过滤器和克隆将被分发 通过我们的CUGI BAC/EST资源中心，以成本回收为基础 （www.genome.clemson.edu）中找到。 BAC图书馆研究与发展：我们将系统地开发 优化和简化BAC图书馆建设程序的技术 为了降低制作图书馆的成本，同时保持质量， 刀片尺寸。此外，还将开发技术， 大小为160 kb或更大。此外，将建立一个强大的工具包 从动物身上提取高质量的兆碱基DNA。这将包括 开发“BIG DNA To-Go”试剂盒，以生产高质量的兆碱基大小的DNA 在需要库的实验室将提供所需组织的位置 来建造图书馆 生物信息学支持：实验室信息管理/条形码系统 将被开发，以帮助跟踪图书馆的生产和分配， 此外，图书馆亦会发展完善的网上图书馆订购系统和数据库。