BAC Library Production /Distribuion for 'Healthy people

描述：(申请人提供)使用 细菌人工染色体(BAC)文库系统已经革命性地和 加快了物理映射、位置克隆和测序的能力 整个基因组。由于克隆大DNA片段的难度很大，只有 少数几个实验室已经能够生产深度覆盖的大型插件 图书馆在例行的基础上。这一瓶颈导致了有限的数量 可供研究社区使用的BAC库的 用于基因发现、调查测序和比较基因组研究。至 增加世界？S北京图书馆建设能力，降低成本， 美国国立卫生研究院发布了一份申请书(RFA.HG-Ol-002)，标题为《BAC图书馆 生产“，这是本申请的主题。 我们电子化计划的具体目标是： BAC图书馆建设：发展BAC图书馆建设能力 36个人类基因组大小(约3000 Mb)10倍基因组相当的BAC文库。在……里面 第一年，我们将以每克隆0.5美元的价格生产6个图书馆，在第二年，12个图书馆 将以每克隆0.375美元的价格制作，并将在第三年18个图书馆 以每克隆0.25美元的价格制作。初始平均插入尺寸将在130之间徘徊 -150kb，我们将努力将我们的平均插入片段大小增加到170-200 KB在随后的几年中。将分发库、过滤器和克隆 通过我们的CUGI BAC/EST资源中心进行成本回收 (www.genome.clemson.edu)。 BAC图书馆研究与发展：我们将系统地发展 优化和简化BAC库构建程序的技术 以降低图书馆的建造成本，同时保持质量和 插入大小。此外，还将开发技术来常规地实现插入 大小为160KB或更大。此外，还将建立一个强大的工具包 从动物身上生产出高质量的百万碱基大小的DNA。这将包括 生产高质量百万碱基DNA的“大DNA外带”试剂盒的研制 关于需要图书馆的实验室将提供所需组织的地点(S) 来建造图书馆。 生物信息学支持：实验室信息管理/条形码系统 将被开发来帮助跟踪图书馆的生产和分发，以及一个 还将开发强大的在线BAC图书馆订购系统和数据库。

DESCRIPTION: (provided by applicant) Cloning large DNA fragments using the bacterial artificial chromosome (BAC) library system has revolutionized and accelerated our ability to physically map, positionally clone and sequence whole genomes. Due to the difficulty in cloning large DNA fragments, only a handful of laboratories have been able to produce deep-coverage large insert libraries on a routine basis. This bottleneck has resulted in a limited number of BAC libraries that are available to the research community that can be used for gene discovery, survey sequencing and comparative genome studies. To increase the world?s BAC library construction capacity and reduce costs, the NIH has posted a request for applications (RFA.HG-Ol-002) entitled "BAC Library Production" which is the subject of this application. The specific aims of our thee-year proposal are: BAC Library Production: Develop BAC library construction capacity to construct 36 human genome size ( about3000 Mb) 10X genome equivalent BAC libraries. In year 1, we will produce 6 libraries at $0.50/clone, in year 2, 12 libraries will be produced at $0.375/clone and in year three 18 libraries will he produced at $0.25/clone. The initial average insert size will hover between 130 - 150 kb and we will strive to increase our average insert sizes to 170 - 200 kb in subsequent years. Libraries, filters and clones will be distributed through our CUGI BAC/EST Resource Center on a cost recovery basis (www.genome.clemson.edu). BAC Library Research and Development: We will systematically develop technologies to optimize and streamline BAC library construction procedures in order to reduce the cost of making libraries while maintaining quality and insert size. Also, technologies will be developed to routinely achieve insert sizes of 160kb or greater. In addition, a robust tool kit will be established to produce high-quality megabase-size DNA from animals. This will include the development of a "BIG DNA To-Go" kit to produce high-quality megabase-size DNA on location where the lab needing a library will provide the tissue(s) required to make the library. Bioinformatics Support: A laboratory information management/bar coding system will be developed to aid in tracking library production and distribution and a robust online BAC library ordering system and database will also be developed.