Measurement Of Cytokines In Cervical Fluids

疱疹病毒和肿瘤之间的关系已经有很好的文献记载，尽管宿主因素在这种关系中所起的作用仍然未知。本项目的主要目的是确定在感染肿瘤型疱疹乳头状瘤病毒（HPV）的妇女的宫颈标本中测量的多分析细胞因子谱是否可以预测随后发展为高级别宫颈上皮内瘤变（CIN）的风险。使用一种称为循环免疫亲和层析（RIC）的技术，检查宫颈液中的细胞因子谱，以确定哪些细胞因子和其他因子在肿瘤过程中出现升高或激活。将使用30个分析物面板来比较来自高度CIN女性样本与致癌HPV感染女性样本和正常对照的细胞因子谱。从这些研究中，希望确定哪些细胞因子或其他因素是预测肿瘤进展的候选因素。一旦这已经确定，这些标记将用于HPV和CIN自然史的前瞻性研究。最初的研究证明了RIC系统的有效性，并表明细胞因子面板在确定不同患者组方面是有用的。进一步的研究表明，RIC系统是可靠的和可重复的。将这一分析系统应用于流行病学实地研究的工作目前正在取得进展。

The relationship between Herpes viruses and neoplasia has been quite well documented although the role played by host factors in this relationship is still unknown. The prime objective of this project to determine whether multi-analyte cytokine profiles measured in cervical specimens, from women infected with oncological types of Herpes Papilloma Virus (HPV), can predict risk of subsequent progression to high-grade cervical intraepithelial neoplasia (CIN). Using a technique called recycling immunoaffinity chromatography (RIC), examination of cytokine profiles in cervical fluid will be performed to determine which cytokines and other factors appear to be elevated or activated during the neoplastic process. 30 analyte panels will be employed to compare cytokine profiles in samples from women with high-grade CIN to samples from women with oncogenic HPV infection and normal controls. From such studies it is hoped to determine which cytokines or other factors are candidates for predicting neoplastic progression. Once this has been established these markers will be used in a prospective study of HPV and CIN natural history. The initial studies demonstrated the efficacy of the RIC system and have shown that cytokine panels are useful in determining the different patient groups. Further studies have demonstrated the RIC system to be reliable and reproducible. Work is now progressing to apply this analytical system to epidemiological field studies.