Assessment Of Multiple Analytes In Women w/ Osteoporosis

骨质疏松症女性多种分析物的评估

• 批准号: 7146066
• 负责人: Terry M. Phillips
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7146066
• 关键词: analytical method; bone density; capillary electrophoresis; circadian rhythms; clinical depression; human tissue; immunoaffinity chromatography; menopause; microarray technology; neuropeptides; osteoporosis; psychosomatic disorders; technology /technique development; women' s health

Bone loss in women pre- and post-menopause is a serious clinical condition affecting a reasonably large proportion of the public. Depression is a compounding condition that has been shown to accentuate pathways leading to bone loss and suggesting a mind-body connection. The POWER study conducted at the NIH Clinical Center is designed to study the interactions of depression and severity of osteoporosis in pre-menopausal women. The DBEPS Ultramicro Analytical Immunochemistry Resource has undertaken to develop a panel of analytical techniques (recycling immunoaffinity chromatography [RIC]; immunoaffinity microarrays; immunoaffinity capillary electrophoresis [ICE]) to study a number of different systemic pathways that possibly may be involved in the connection between osteoporosis and depression. Cohorts of both normal and depressed age-matched women have been recruited into the study, double-blinded and the codes broken after the analyses. Samples were taken on three occasions during which 24 one-hour samples were taken and analyzed for 45 different analytes. Although the sample analyses have now been successfully completed, the data analysis is still in progress due to the extremely large number of data points generated. Preliminary examination of the data has indicated circadian rhythms for many of the different cytokines and growth factors measured. The data also indicates that the depressed individuals can be classified into two groups according to their analyte patterns. Preliminary analysis has indicated a strong correlation between the presence of pain-associated neuropeptides and changes in bone density.

绝经前和绝经后妇女的骨质丢失是一种严重的临床情况，影响到相当大比例的公众。抑郁症是一种复合性疾病，已被证明会加剧导致骨质流失的途径，并提示精神和身体的联系。在美国国立卫生研究院临床中心进行的POWER研究旨在研究绝经前女性抑郁与骨质疏松严重程度之间的相互作用。DBEPS超微分析免疫化学资源已着手开发一组分析技术(循环免疫亲和层析[RIC]；免疫亲和微阵列；免疫亲和毛细管电泳法[ICE])，以研究可能涉及骨质疏松症和抑郁症之间联系的一些不同的全身途径。年龄匹配的正常女性和抑郁女性都被招募到这项研究中，分析后采用双盲方法，密码被破译。采集了三次样品，在此期间采集了24个一小时的样品，并对45种不同的分析物进行了分析。虽然样本分析现已成功完成，但由于产生的数据点数量极大，数据分析仍在进行中。对数据的初步检查表明，所测量的许多不同细胞因子和生长因子的昼夜节律。数据还表明，抑郁的个体可以根据他们的分析物模式分为两组。初步分析表明，疼痛相关神经肽的存在与骨密度的变化有很强的相关性。