Assessment Of Multiple Analytes In Women w/ Osteoporosis

绝经前和绝经后妇女骨质流失是一种严重的临床状况，影响相当大比例的公众。抑郁症是一种复杂的情况，它被证明会加剧导致骨质流失的途径，并表明与身心有关。在美国国立卫生研究院临床中心进行的POWER研究旨在研究绝经前妇女抑郁和骨质疏松严重程度的相互作用。DBEPS超微分析免疫化学资源已经开始开发一组分析技术（循环免疫亲和层析[RIC]；免疫亲和微阵列；免疫亲和毛细管电泳[ICE]）来研究可能涉及骨质疏松症和抑郁症之间联系的许多不同的系统途径。这项研究招募了年龄相仿的正常和抑郁女性，采用双盲法，分析后密码被破译。样品采集了三次，其中24个一小时的样品被采集并分析了45种不同的分析物。虽然现在样本分析已经成功完成，但是由于产生的数据点非常多，数据分析还在进行中。对数据的初步检查表明，测量了许多不同细胞因子和生长因子的昼夜节律。数据还表明，抑郁症患者可以根据他们的分析模式分为两组。初步分析表明，疼痛相关神经肽的存在与骨密度的变化之间存在很强的相关性。

Bone loss in women pre- and post-menopause is a serious clinical condition affecting a reasonably large proportion of the public. Depression is a compounding condition that has been shown to accentuate pathways leading to bone loss and suggesting a mind-body connection. The POWER study conducted at the NIH Clinical Center is designed to study the interactions of depression and severity of osteoporosis in pre-menopausal women. The DBEPS Ultramicro Analytical Immunochemistry Resource has undertaken to develop a panel of analytical techniques (recycling immunoaffinity chromatography [RIC]; immunoaffinity microarrays; immunoaffinity capillary electrophoresis [ICE]) to study a number of different systemic pathways that possibly may be involved in the connection between osteoporosis and depression. Cohorts of both normal and depressed age-matched women have been recruited into the study, double-blinded and the codes broken after the analyses. Samples were taken on three occasions during which 24 one-hour samples were taken and analyzed for 45 different analytes. Although the sample analyses have now been successfully completed, the data analysis is still in progress due to the extremely large number of data points generated. Preliminary examination of the data has indicated circadian rhythms for many of the different cytokines and growth factors measured. The data also indicates that the depressed individuals can be classified into two groups according to their analyte patterns. Preliminary analysis has indicated a strong correlation between the presence of pain-associated neuropeptides and changes in bone density.