Assessment Of Multiple Analytes In Women With Osteoporosis And Depression

绝经前和绝经后妇女的骨丢失是一种严重的临床状况，影响相当大比例的公众。 抑郁症是一种复合性疾病，已被证明会加重导致骨质流失的途径，并表明身心联系。 在NIH临床中心进行的POWER研究旨在研究绝经前妇女抑郁症和骨质疏松症严重程度的相互作用。 超微免疫诊断小组，NIBIB已着手开发一组分析技术（回收免疫亲和色谱RIC;免疫亲和微阵列;免疫亲和毛细管电泳ICE），以研究可能参与骨质疏松症和抑郁症之间联系的许多不同的系统途径。 正常和抑郁年龄匹配的妇女队列已被招募到双盲研究。 仅在分析后破盲。 在三种情况下采集样品，在此期间采集24个一小时样品并分析45种不同的分析物。 虽然样本分析现已成功完成，但由于产生了大量数据点，数据分析仍在进行中。 初步检查的数据表明，昼夜节律的许多不同的细胞因子和生长因子的测量。 数据还表明，抑郁症患者可以分为两组，根据他们的分析模式。 初步分析表明，疼痛相关神经肽的存在与骨密度的变化之间有很强的相关性。 最近的研究集中在开发使用微创或非侵入性程序测量免疫应激生物标志物的技术。 已经研究了一种市售的汗液贴片作为非侵入性24小时收集装置。 该贴片基本上是一个大的粘性绷带，在正常活动期间应用于受试者的皮肤并留在原位24小时。 移除贴片并提取收集的汗液。 经过大量的实验，人们发现，真空提取装置提供了最有效的手段来回收所需的体液。 还从测试受试者中采集血浆样本，以比较分析物浓度。 最初的研究表明，汗液的恢复与血浆浓度相当，并且可以使用现有的分析技术轻松测量一组神经免疫标记物。

Bone loss in women pre- and post-menopause is a serious clinical condition affecting a reasonably large proportion of the public. Depression is a compounding condition that has been shown to accentuate pathways leading to bone loss and suggests a mind-body connection. The POWER study conducted at the NIH Clinical Center is designed to study the interactions of depression and severity of osteoporosis in pre-menopausal women. The Ultramicro Immunodiagnostics group, NIBIB has undertaken to develop a panel of analytical techniques (recycling immunoaffinity chromatography RIC; immunoaffinity microarrays; immunoaffinity capillary electrophoresis ICE) to study a number of different systemic pathways that possibly may be involved in the connection between osteoporosis and depression. Cohorts of both normal and depressed age-matched women have been recruited into the double-blinded study. Codes were broken only after the analyses. Samples were taken on three occasions, during which 24 one-hour samples were taken and analyzed for 45 different analytes. Although the sample analyses have now been successfully completed, the data analysis is still in progress, due to the extremely large number of data points generated. Preliminary examination of the data has indicated circadian rhythms for many of the different cytokines and growth factors measured. The data also indicate that the depressed individuals can be classified into two groups according to their analyte patterns. Preliminary analysis has indicated a strong correlation between the presence of pain-associated neuropeptides and changes in bone density. Recent studies have focused on the development of techniques for measuring immune-stress biomarkers using minimally or non-invasive procedures. A commercially available sweat patch has been studied as a non-invasive 24-hour collection device. The patch, essentially a large adhesive bandage, is applied to the subjects skin and left in place for 24 hours during normal activities. The patch is removed and the collected sweat extracted. After considerable experimentation, it was found that a vacuum extraction device provided the most efficient means to recover the desired body fluid. Plasma samples were also drawn from the test subjects in order to compare analyte concentrations. The initial studies have shown that sweat recovery compares well with plasma concentrations and that a panel of neuro-immune markers of stress and inflammation can easily be measured using the analytical techniques already in place.