Assessment Of Multiple Analytes In Women With Osteoporosis And Depression

绝经前和绝经后妇女的骨质丢失是一种严重的临床情况，影响到相当大比例的公众。抑郁症是一种复合性疾病，已被证明会加剧导致骨质流失的途径，并暗示着一种精神和身体的联系。在美国国立卫生研究院临床中心进行的POWER研究旨在研究绝经前女性抑郁与骨质疏松严重程度之间的相互作用。超微免疫诊断组NIBIB已着手开发一组分析技术(循环免疫亲和层析RIC、免疫亲和微阵列、免疫亲和毛细管电泳法)，以研究可能涉及骨质疏松症和抑郁症之间联系的许多不同的系统途径。年龄匹配的正常和抑郁女性都被招募到这项双盲研究中。只有在分析之后，密码才被破译。采集了三次样品，在此期间采集了24个一小时的样品，并对45种不同的分析物进行了分析。尽管样本分析现已成功完成，但由于产生的数据点数量极大，数据分析仍在进行中。对数据的初步检查表明，所测量的许多不同细胞因子和生长因子的昼夜节律。数据还表明，抑郁的人可以根据他们的分析物模式分为两组。初步分析表明，疼痛相关神经肽的存在与骨密度的变化有很强的相关性。 最近的研究集中在使用最小或非侵入性程序测量免疫应激生物标记物的技术的发展。一种商业化的汗贴已经被研究为一种非侵入性的24小时收集设备。这种贴片本质上是一块大胶带，涂在受试者的皮肤上，在正常活动期间放置24小时。取下贴片，提取收集到的汗水。经过大量试验，人们发现真空抽出装置是回收所需体液的最有效方法。为了比较分析物的浓度，还从测试对象中提取了血浆样本。初步研究表明，汗液回收量与血浆浓度相当，而且可以使用现有的分析技术轻松测量一组反映压力和炎症的神经免疫标记物。

Bone loss in women pre- and post-menopause is a serious clinical condition affecting a reasonably large proportion of the public. Depression is a compounding condition that has been shown to accentuate pathways leading to bone loss and suggests a mind-body connection. The POWER study conducted at the NIH Clinical Center is designed to study the interactions of depression and severity of osteoporosis in pre-menopausal women. The Ultramicro Immunodiagnostics group, NIBIB has undertaken to develop a panel of analytical techniques (recycling immunoaffinity chromatography RIC; immunoaffinity microarrays; immunoaffinity capillary electrophoresis ICE) to study a number of different systemic pathways that possibly may be involved in the connection between osteoporosis and depression. Cohorts of both normal and depressed age-matched women have been recruited into the double-blinded study. Codes were broken only after the analyses. Samples were taken on three occasions, during which 24 one-hour samples were taken and analyzed for 45 different analytes. Although the sample analyses have now been successfully completed, the data analysis is still in progress, due to the extremely large number of data points generated. Preliminary examination of the data has indicated circadian rhythms for many of the different cytokines and growth factors measured. The data also indicate that the depressed individuals can be classified into two groups according to their analyte patterns. Preliminary analysis has indicated a strong correlation between the presence of pain-associated neuropeptides and changes in bone density. Recent studies have focused on the development of techniques for measuring immune-stress biomarkers using minimally or non-invasive procedures. A commercially available sweat patch has been studied as a non-invasive 24-hour collection device. The patch, essentially a large adhesive bandage, is applied to the subjects skin and left in place for 24 hours during normal activities. The patch is removed and the collected sweat extracted. After considerable experimentation, it was found that a vacuum extraction device provided the most efficient means to recover the desired body fluid. Plasma samples were also drawn from the test subjects in order to compare analyte concentrations. The initial studies have shown that sweat recovery compares well with plasma concentrations and that a panel of neuro-immune markers of stress and inflammation can easily be measured using the analytical techniques already in place.