In Vitro Differentiation of Inner Ear Hair Cells

内耳毛细胞的体外分化

• 批准号: 6650985
• 负责人: Patricia M. White
• 金额: $ 4.64万
• 依托单位: HOUSE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6650985
• 关键词: biological signal transduction; bromodeoxyuridine; cell cycle; cell differentiation; deafness; ear hair cell; flow cytometry; genetically modified animals; green fluorescent proteins; immunofluorescence technique; laboratory mouse; labyrinth; nervous system regeneration; tissue /cell culture

DESCRIPTION (provided by applicant): Sensorineural deafness in adults is primarily due to the permanent loss of sensory hair cells in the inner ear. In this regard, mammals differ from lower vertebrates, which can regenerate sensory hair cells from adjacent supporting cells after injury. It is not known whether this difference arises from a lack of sensory developmental capacity in mammalian support cells, or from a lack of some endogenous stimulus in mammals to provoke regeneration after otic injury. Experiments described in this proposal will mark embryonic sensory and support cell precursors with a fluorescent transgene. These precursors will be purified from embryos using fluorescence activated cell sorting (FACS) and differentiated in vitro, using a recently developed culture system. This system will also be used to isolate and differentiate post-mitotic support cells from embryos and from post-natal animals. From these experiments, it will be determined whether post-mitotic mammalian support cells are able to generate sensory hair cells, and if so, how long in development this capacity persists.

描述（由申请人提供）：成人感音神经性耳聋为