In Vitro Differentiation of Inner Ear Hair Cells

内耳毛细胞的体外分化

• 批准号: 6650985
• 负责人: Patricia M. White
• 金额: $ 4.64万
• 依托单位: HOUSE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6650985
• 关键词: biological signal transduction; bromodeoxyuridine; cell cycle; cell differentiation; deafness; ear hair cell; flow cytometry; genetically modified animals; green fluorescent proteins; immunofluorescence technique; laboratory mouse; labyrinth; nervous system regeneration; tissue /cell culture

DESCRIPTION (provided by applicant): Sensorineural deafness in adults is primarily due to the permanent loss of sensory hair cells in the inner ear. In this regard, mammals differ from lower vertebrates, which can regenerate sensory hair cells from adjacent supporting cells after injury. It is not known whether this difference arises from a lack of sensory developmental capacity in mammalian support cells, or from a lack of some endogenous stimulus in mammals to provoke regeneration after otic injury. Experiments described in this proposal will mark embryonic sensory and support cell precursors with a fluorescent transgene. These precursors will be purified from embryos using fluorescence activated cell sorting (FACS) and differentiated in vitro, using a recently developed culture system. This system will also be used to isolate and differentiate post-mitotic support cells from embryos and from post-natal animals. From these experiments, it will be determined whether post-mitotic mammalian support cells are able to generate sensory hair cells, and if so, how long in development this capacity persists.

描述（申请人提供）：成人的感觉性耳聋是 主要是由于内耳中的感觉毛细胞永久丧失。在 这方面，哺乳动物与下脊椎动物有所不同，后者可以再生感觉 受伤后，来自相邻支撑细胞的毛细胞。不知道是否 这种差异是由于缺乏感觉的发展能力 哺乳动物支持细胞，或由于缺乏哺乳动物的内源性刺激 在耳损伤后促进再生。在此描述的实验 建议将标记胚胎感觉和支持细胞前体 荧光转基因。这些前体将从胚胎中使用 荧光激活的细胞分选（FACS）并使用A分化 最近开发的文化系统。该系统也将用于隔离和 区分有丝分裂后的支持细胞与胚胎和产后分离 动物。从这些实验中，将确定是否有丝分裂 哺乳动物的支持细胞能够产生感觉毛细胞，如果是， 长期以来，这种能力仍然存在。