Protein targeting and secretion in Toxoplasma

弓形虫中的蛋白质靶向和分泌

• 批准号: 6700794
• 负责人: BORIS STRIEPEN
• 金额: $ 25.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-15 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6700794
• 关键词: Toxoplasma gondii; biological signal transduction; flow cytometry; fluorescent dye /probe; gene complementation; gene deletion mutation; genetic library; genetic mapping; granule; host organism interaction; organelles; parasitism; protein transport; reporter genes; secretion; vesicle /vacuole

DESCRIPTION (provided by applicant): Toxoplasma gondii is a wide-spread opportunistic pathogen causing severe encephalitis in AIDS patients. T. gondii is also an experimental model system for the study of related apicomplexan parasites important in AIDS including Cryptosporidium, Cyclospora, Isospora, and Plasmodium the causative agent of malaria. Replication of apicomplexan parasites takes place only inside the cells of its host, within a specialized compartment formed during invasion - the parasitophorous vacuole. Protein secretion is tightly associated with host-cell invasion and establishment/maintenance of the parasitophorous vacuole. As invasion is essential to parasite replication these steps might also pesent potential targets for anti-parasite drug or vaccine development. This proposal aims to study the role of protein secretion and targeting in host cell invasion and vacuole maturation. The secretory apparatus in T gondii is highly diversified, with secretion occurring from three sets of organelles: micronemes, rhoptries, and dense granules. We will characterize protein trafficking to and from these organelles using in vivo reporter genes. Sequence mapping using deletion analysis and more subtle point-mutations will permit us to establish the molecular signals involved in this sorting process. Conditional mutants will be isolated in a phenotypic screen enriching for the accumulation of a secretory reporter. Isolated mutant parasites will be used to establish if secretion is essential or coincidental with the invasion event. Mutant analysis will also provide critical information towards the function of the individual secretory organelles involved.

描述（申请人提供）：刚地弓形虫是一种广泛传播的弓形虫

项目成果

Genomics meets transgenics in search of the elusive Cryptosporidium drug target.

基因组学与转基因技术相结合，寻找难以捉摸的隐孢子虫药物靶点。

• DOI: 10.1016/j.pt.2004.06.003
• 发表时间: 2004
• 期刊: Trends in parasitology
• 影响因子: 9.6
• 作者: Striepen,Boris;Kissinger,JessicaC
• 通讯作者: Kissinger,JessicaC