Reduction of Alkylating Quinones and Nitroarenes

描述（由申请人提供）：已知生物还原烷基化醌和硝基芳烃的中间体在缺氧条件下相应的母体化合物还原后与DNA共价结合。这种行为已被发现在实体瘤的治疗中是有价值的。超声诱导的空化可以使这些醌类和硝基芳烃类化合物减少，从而达到更好的肿瘤/正常细胞选择性比，因为超声可以直接照射到选定的组织。因此，本AREA应用的一般目的之一是确定烷基化醌和硝基芳烃是否通过醌和硝基芳烃的声波分解而被一个或多个电子还原。第二个主要目的是确定DNA是否在超声波照射含有DNA和这些生物还原化合物的溶液时烷基化。与DNA的共价结合将在uph -医学科学校区进行检测，这将开启uph和upr -医学科学校区教师之间的合作。本科学生将参与该项目，从而提高他们从事生物医学研究事业的资格。

DESCRIPTION (provided by applicant): Intermediates of bioreductive alkylating quinones and nitroarenes are known to bind covalently to DNA after reduction of the corresponding parent compound under hypoxia. Such a behavior has been found to be valuable in the treatment of solid tumors. Better tumor/normal cell selectivity ratio should be achieved if these quinones and nitroaromatics could be reduced through ultrasound-induced cavitation since ultrasound irradiation can be directed to the selected tissue. Thus, one of the general aims of this AREA application is to determine if alkylating quinones and nitroaromatics are reduced by one or more electrons through the sonolyses of these quinones and nitroarenes. A second major aim is to determine if DNA is alkylated during ultrasound irradiation of solutions containing DNA and these bioreductive compounds. Covalent binding to DNA will be detected at UPR-Medical Sciences campus, which will open collaboration between faculty of UPRH and UPR-Medical Sciences Campus. Undergraduate students will participate in this project, thus improving their qualifications to pursue biomedical research careers.