UPRH Biomedical Research Improvement Program

UPRH 生物医学研究改进计划

• 批准号: 7982352
• 负责人: ANTONIO E ALEGRIA
• 金额: $ 24.68万
• 依托单位: UNIVERSITY OF PUERTO RICO AT HUMACAO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-11-15 至 2011-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7982352
• 关键词: Amines; Amino Acid Sequence; Biomedical Research; Chemistry; Collaborations; Communities; Crystallization; Dependence; Development; Enzymes; Equipment; Extramural Activities; Faculty; Funding; Goals; Human Resources; Institution; International; Label; Maintenance; Membrane Proteins; Methods; Natural Sciences; Neurologic; Organic solvent product; Peer Review; Phenothiazines; Preparation; Production; Productivity; Proteins; Publications; Puerto Rico; Quinones; Research; Research Activity; Research Personnel; Research Project Grants; Research Proposals; Role; Solvents; Structure; Students; System; Time; Toxic effect; Universities; design; enzyme activity; improved; monolayer; operation; programs; protein folding; tumor

The general aim of this SCORE application is the upgrade of physical and human resources at the University of Puerto Rico at Humacao (UPRH) in order to increase and improve its biomedical research activity. The following goals are set in pursuing this major aim: (1) to increase the biomedical research capacity at UPRH, (2) to increase the number of natural sciences faculty at UPRH involved in biomedical research and the number of biomedical research collaborations with research-intensive institutions, (3) to increase the number of biomedical research collaborations with researchintensive institutions, (4) to increase the number of submitted biomedical research grant applications from UPRH to non-MBRS extramural biomedical research agencies and (5) to increase the biomedical research productivity at UPRH. Goal (1) will be achieved by hiring a full-time technician for the operation and maintenance of our most sophisticated MBRS-funded equipment. Goal (2) will be accomplished starting with the addition of an electrochemist to our SCORE project. This investigator is submitting a biomedical research proposal in this application dealing with the developing of a new system for membrane protein crystallization, using the self-assembled monolayers methods (SAMs). Five other research investigators from the Chemistry department are also submitting research proposals in this application. One of these Chemistry projects deals with a study of the solvent dependence of enzyme enantioselectivity with the purpose of understanding and improving enzyme's activity, stability and enantioselectivity in organic solvents for the small and large scale synthesis of bio-relevant compounds. A second project looks for the developing of new phenothiazine derivatives (neurologically-active compounds) with less undesirable photocytotoxic effects, as well as understanding the mechanisms underlying in these photocytotoxic effects. A third project proposes the design of improved methods for the estereoselective preparation of diverse kind of amines with potential neurological effects. A fourth Chemistry project deals with a systematic study of different (3-hairpins structures in proteins using isotopic labelling along the primary amino acid sequence to understand protein folding dynamics. A fifth Chemistry subproject will study quinone roles in NO reduction and production enhancements and tumor-targeted toxicity of alkylating quinones. It is expected that the development of these projects will improve the possibilities of producing more research proposal applications to other extramural agencies, more research collaborations and an increase in peer reviewed publications with the concomitant recognition of our faculty and students in the international biomedical research community.

本次成绩申请的总体目标是提升波多黎各大学的体力和人力资源 RICO在Humacao(UPRH)工作，以增加和改进其生物医学研究活动。设定了以下目标 在实现这一主要目标方面：(1)提高大学公共卫生研究所的生物医学研究能力，(2)增加 UPRH参与生物医学研究的自然科学教员和生物医学研究合作的数量 与研究密集型机构合作，(3)增加与研究密集型生物医学研究合作的数量 机构，(4)将UPRH提交的生物医学研究资助申请增加到 非MBR校外生物医学研究机构和(5)提高UPRH的生物医学研究生产力。 目标(1)将通过聘请一名全职技术人员来操作和维护我们最先进的 MBR资助的设备。目标(2)将通过在我们的分数中增加一名电化学家开始实现 项目。这位研究人员在这份申请中提交了一份生物医学研究提案，涉及开发 利用自组装单分子膜方法(SAMS)建立了膜蛋白结晶的新体系。其他五个人 化学系的研究人员也在此申请中提交研究建议。其中之一 这些化学项目涉及酶对映体选择性的溶剂依赖性的研究，目的是 了解和提高酶在有机溶剂中的活性、稳定性和对映体选择性 生物相关化合物的大规模合成。第二个项目是寻找新的吩噻嗪的开发 具有较少不良光细胞毒性作用的衍生物(神经活性化合物)，以及理解 这些光细胞毒作用的潜在机制。第三个项目提出了改进的方法的设计 具有潜在神经作用的各种胺的酯选择性制备。第四个化学项目 利用同位素标记对蛋白质中不同的(3-发夹)结构进行了系统的研究 氨基酸序列，以了解蛋白质折叠动力学。第五个化学子项目将研究苯二酚在 烷基化对苯二酚的NO还原和生产促进作用及肿瘤靶向性毒性。预计将会有 这些项目的发展将提高产生更多研究提案应用到其他国家的可能性 外部机构，更多的研究合作，以及同行评议出版物的增加 我们的教职员工和学生在国际生物医学研究界得到了认可。

项目成果

Sonochemistry of quinones in argon-saturated aqueous solutions: enhanced cytochrome c reduction.

氩饱和水溶液中醌的声化学：增强细胞色素 c 还原。

• DOI: 10.1021/tx990064r
• 发表时间: 1999
• 期刊: Chemical research in toxicology.
• 作者: Lawson,RC;Ferrer,A;Flores,W;Alegria,AE
• 通讯作者: Alegria,AE

Photooxidation mechanism of levomepromazine in different solvents.

左美丙嗪在不同溶剂中的光氧化机理。

• DOI: 10.1111/php.12147
• 发表时间: 2013
• 期刊: Photochemistry and photobiology
• 影响因子: 3.3
• 作者: Piñero-Santiago,LuisE;García,Carmelo;Lhiaubet-Vallet,Virginie;Trzcionka,Jerome;Oyola,Rolando;Torres,Karen;Leguillú,Jaysika;Miranda,MiguelA
• 通讯作者: Miranda,MiguelA

A 1,3,2-oxazaborolidine dimer derived from (S)-alpha,alpha-diphenylprolinol.

衍生自 (S)-α,α-二苯基脯氨醇的 1,3,2-恶唑硼烷二聚体。

• DOI: 10.1107/s0108270104000666
• 发表时间: 2004
• 期刊: Acta crystallographica. Section C, Crystal structure communications
• 作者: Ortiz-Marciales,Margarita;DeJesús,Melvin;González,Eduvigis;Raptis,RaphaelG;Baran,Peter
• 通讯作者: Baran,Peter

Role of membrane charge and semiquinone structure on naphthosemiquinone derivatives and 1,4-benzosemiquinone disproportionation and membrane-buffer distribution coefficients.

膜电荷和半醌结构对萘半醌衍生物和 1,4-苯并半醌歧化和膜缓冲液分配系数的作用。

• DOI: 10.1080/10715760100301541
• 发表时间: 2001
• 期刊: Free radical research
• 影响因子: 3.3
• 作者: Alegría,AE;Santiago,G;Lópes,M;Rosario,BI;Cordones,E
• 通讯作者: Cordones,E

SPECTROSCOPIC AND ELECTROCHEMICAL PROPERTIES OF 2-AMINOPHENOTHIAZINE.

2-氨基吩噻嗪的光谱和电化学性质。

• DOI: 10.1016/j.jphotochem.2008.02.028
• 发表时间: 2008
• 期刊: Journal of photochemistry and photobiology. A, Chemistry
• 作者: Piñero,Luis;Calderón,Xiomara;Rodríguez,Juan;Nieves,Ileana;Arce,Rafael;García,Carmelo;Oyola,Rolando
• 通讯作者: Oyola,Rolando