A Lab-on-PCB biomedical diagnostic platform for on-chip protein preconcentration.

用于片上蛋白质预浓缩的 Lab-on-PCB 生物医学诊断平台。

• 批准号: 2281761
• 金额: --
• 依托单位: University of Bath
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2281761
• 关键词: Lab; PCB; biomedical; diagnostic; platform

For point-of-care protein-based diagnosis, the sensitivity of a device is limited by the small quantities of proteins contained within a sample. The aim of this project is to utilise a method of protein separation termed 'isoelectric focusing' (IEF) along with electrochemical control of the local pH to perform simultaneous purification, preconcentration and separation of proteins within a small sample based on their isoelectric points (PI). The finished device will combine these techniques on a printed circuit board (PCB) platform, lab-on-PCB, and will enable the efficient diagnosis for a broad range of health conditions, ranging from cancer to infectious disease.Isoelectric focusing ordinarily refers to the macroscopic separation of proteins along a pH gradient due to their isoelectric point (the pH at which the carboxyl/amine groups of a protein are neutralised) with an applied electric field. By producing a mechanism through which the local pH may be precisely controlled and maintained, the proposed device will be able to perform isoelectric focusing of specific proteins based on the current or voltage supplied to electrodes. In this innovative approach, we aim to miniaturise these processes and utilise microfluidic techniques within a PCB format to preconcentrate proteins for analysis.The foreseeable landmark tasks will be as follows:Assess the current state of the art for both techniques;Simulate existing techniques using COMSOL Multiphysics;Perform experiments based on the results of the simulations;Integrate the knowledge gained into designing, simulating, building and testing a prototype which combines IEF with a method of local pH control in a miniaturised PCB platform.

对于基于护理点蛋白质的诊断，设备的灵敏度受到样本中包含的少量蛋白质的限制。该项目的目的是利用一种称为等电聚焦(IEF)的蛋白质分离方法以及对局部pH的电化学控制，根据蛋白质的等电点(PI)同时对小样本中的蛋白质进行纯化、预浓缩和分离。完成后的设备将在印刷电路板(PCB)平台上结合这些技术，并将能够对从癌症到传染病的广泛健康状况进行有效诊断。等电聚焦通常指的是蛋白质沿等电点(蛋白质的羧基/胺基团被中和的pH值)和外加电场沿pH梯度进行的宏观分离。通过制造一种可以精确控制和维持局部pH的机制，所提出的设备将能够根据提供给电极的电流或电压对特定蛋白质进行等电聚焦。在这种创新的方法中，我们的目标是将这些过程微型化，并在印刷电路板格式中利用微流控技术来预浓缩蛋白质进行分析。可预见的里程碑式任务如下：评估这两种技术的当前技术水平；使用COMSOL多物理模拟现有技术；基于模拟结果进行实验；将所获得的知识整合到设计、模拟、构建和测试原型中，该原型将IEF与微型印刷电路板平台中的局部pH控制方法相结合。