Super-resolution video rate imaging of photosynthetic membrane dynamics in plants and algae

植物和藻类光合膜动力学的超分辨率视频速率成像

• 批准号: 2282818
• 金额: --
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2282818
• 关键词: Super; resolution; video; rate; imaging

Remarkable images of the internal workings of plant chloroplasts and algaeprovided by structured illumination microscopy suggest a major role forphotosynthetic membrane morphological changes in the adaptation to changinglight intensity. Our study of this largely unexplored phenomenon has revealedrapid dynamics that help the plant match the conversion of light to chemicalenergy with its consumption for CO 2 fixation into biomass. Now we have theability to image these dynamics in real time at super-resolution for the first timeusing a breakthrough microscopy technology. The super-resolutionprogrammable array microscope (SR-PAM), can adapt during imaging tooptimise signal to noise and resolution. The adaptability of the system meansthat we can push super-resolution further in to deep samples. Utilising thisexciting new microscopy technique we will investigate how the thylakoidmembrane dynamics regulate photosynthesis. The results of this study have thepotential to provide an unexpected route to improving photosynthesis forenhanced crop and biofuel production.

结构照明显微镜提供的植物叶绿体和藻类内部工作的显着图像表明，光合膜形态变化在适应变化的光强度中起着重要作用。我们对这一基本上未被探索的现象的研究揭示了快速的动力学，帮助植物将光转化为化学能与其消耗的CO2固定为生物质相匹配。现在，我们有能力在超分辨率下对这些动态进行真实的成像，这是第一次使用突破性的显微镜技术。超分辨率可编程阵列显微镜（SR-PAM）可以在成像过程中进行调整，以优化信噪比和分辨率。系统的适应性意味着我们可以将超分辨率进一步推向更深的样本。利用这种令人兴奋的新显微镜技术，我们将研究类囊体膜动力学如何调节光合作用。这项研究的结果有可能提供一个意想不到的途径，以提高作物和生物燃料生产的光合作用。