Rapid Determination of Viability of Anthrax Spores

炭疽孢子活力的快速测定

• 批准号: 6662558
• 负责人: Rashid Bashir
• 金额: $ 22.5万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-25 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6662558
• 关键词: Bacillus anthracis; SDS polyacrylamide gel electrophoresis; bacterial proteins; bacterial toxins; bioengineering /biomedical engineering; biohazard detection; biomedical equipment development; biosensor device; biotechnology; bioterrorism /chemical warfare; electron microscopy; fluorescence microscopy; high throughput technology; microarray technology; microorganism classification; nanotechnology; polymerase chain reaction; spores

DESCRIPTION (provided by applicant): Micro- and nano-systems technology has found increasing use in a wide variety of biomedical applications, including detection and characterization of biological entities. The devices used for such applications are broadly referred to as 'bio-chips' and for example in the case of DNA detection, have even been commercialized. One area of research that has become increasingly important but not very well studied is the handling, manipulation, and characterization of very few cells and micro-organisms using biomedical micro-electro-mechanical-systems technology (BioMEMS). The detection of very low cell concentrations from samples of bodily fluids, tissue samples, soil, water, and food is a challenge that has not been fully realized. The goal of such an effort should be to handle, detect, and characterize a single cell or microorganism, and micro-devices are ideally suited for such studies. In addition, reducing the time-to-result to be able to perform "point-of-use" analysis is also very important. Such endeavors can not only yield very important scientific results but can also be used immediately in practical diagnostic applications in the health and food industry and in biological warfare and hazard prevention systems. This project brings together interdisciplinary researchers from the fields of micro/nano-systems technology and microbiology with the knowledge of Bacillus anthracis (anthrax) to further the state-of-the-art in micro-scale detection and identification of Bacillus anthracis. The knowledge developed herein will also apply to other microorganisms. The PIs have developed novel technologies that serve as the basis and starting point for continued state-of-the-art research. Micro-devices will be developed to rapidly detect the viability of the spores upon germination within one doubling cycle of the organisms, providing an electronic output. The devices will also have built-in electronic filters to concentrate the spores and cells inside the biochips at the detection sites. In parallel, biological analysis will be performed to identify the spore coat proteins, especially those novel to B. anthracis spores. These proteins are surface localized and thus useful for spore detection. The eventual system is envisioned to capture only the spores or pathogens of interested inside the bio-chips using these surface protein receptors, concentrate these microorganisms inside this chip, and electronically detect their viability and germination, while reducing the total time to result to less than possible by any other technology.

描述（由申请人提供）：微型和纳米系统技术发现在各种生物医学应用中的使用越来越多，包括生物实体的检测和表征。用于此类应用的设备被广泛称为“生物芯片”，例如，在DNA检测的情况下，甚至已被商业化。使用生物医学微电动机械系统技术（BioMems）的处理，操纵和表征的处理，操纵和表征很少，但研究领域变得越来越重要，但研究不太深入。从体液，组织样品，土壤，水和食物样品中检测出非常低的细胞浓度是尚未完全实现的挑战。这种努力的目的应该是处理，检测和表征单个细胞或微生物，而微设备非常适合此类研究。此外，减少能够执行“使用点”分析的时间的时间也非常重要。这样的努力不仅可以产生非常重要的科学结果，而且还可以立即用于卫生和食品工业以及生物战和危害预防系统中的实际诊断应用中。 该项目将来自微/纳米系统技术和微生物学领域的跨学科研究人员汇集了炭疽芽孢杆菌（炭疽菌）的知识，以进一步在微型检测和拟杆菌的识别中进一步识别。本文所开发的知识也将适用于其他微生物。 PI开发了新型技术，这些技术是持续最新研究的基础和起点。将开发微设备以在一个生物体的双倍周期内发芽后快速检测孢子的生存能力，从而提供电子输出。这些设备还将具有内置的电子过滤器，以将孢子和细胞集中在检测位点的生物芯片内。同时，将进行生物学分析，以鉴定孢子涂层蛋白，尤其是那些对炭疽芽孢杆菌孢子的新颖的蛋白。这些蛋白质是表面局部化的，因此可用于孢子检测。设想最终的系统仅捕获使用这些表面蛋白受体在生物芯片中感兴趣的孢子或病原体，将这些微生物集中在此芯片中，并电子地检测到它们的生存力和发芽，同时减少其他技术的总时间，从而减少总时间。