Belowground carbon sequestration potential of apple trees

苹果树地下固碳潜力

• 批准号: 2289429
• 金额: --
• 依托单位: University of Reading
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2289429
• 关键词: Belowground; carbon; sequestration; potential; apple

ObjectivesThis project will expand our understanding of the associations between terrestrial C sequestration capacity and genotype-specific apple rootstock traits, including recalcitrance, rhizodeposition and the associated rhizosphere microbial communities. This knowledge will then enable growers to select rootstocks with greater ecosystem service capacity, in terms of their C storage abilities, which will benefit soil fertility and promote the capture of atmospheric CO2. In turn, policy makers may incorporate this information into environmental reward payment schemes.ApproachesThis project will assess a selection of widely-used apple rootstock cultivars for their C-sequestering abilities by analysing three key components:Physical root measurements: Root recalcitrance will be assessed using biochemical methods to quantify various compounds (e.g. total C, soluble phenolics, condensed tannins, acid-insoluble fractions (lignin)). Additional measurements will be made for root thickness, decomposition rate, and root depth distribution using rhizotrons, intact cores (Dornbush, et al., 2002) and incubation of roots in microcosms.Root exudate biochemical analysis: Rhizodeposit collection may be done either by collection of percolated (run-off) water from pot-grown rootstocks (Leisso, et al., 2017), or by dipping roots of axenically-grown rootstocks in distilled water containing silver (Ag+) as described and evaluated by Gransee & Wittenmayer (2000). Primary (sugars, organic acids) and secondary (phenolics) metabolites will be analysed by high performance liquid chromatography (HPLC) or gas chromatography-mass spectrometry (GC-MS).Rhizosphere community analysis: Recent advances in high-throughput sequencing methods have greatly enhanced microbial community analysis. The bacterial and fungal composition of the rhizosphere community will be investigated via amplification of the universal 16S rRNA gene (bacteria) and ITS sequences (fungi). In particular, the project will focus on functional groups related to C cycling, such as methanotrophs (methane producers), methanogens (methane consumers), and AMF.

目的本项目将扩大我们对陆地固碳能力与基因型特异性苹果砧木性状之间关系的理解，包括植物的吸收能力、根际沉积和相关的根际微生物群落。这些知识将使种植者能够选择具有更大生态系统服务能力的砧木，就其C储存能力而言，这将有利于土壤肥力并促进大气CO2的捕获。反过来，政策制定者可能会将这些信息纳入环境奖励支付schemes.ApproachesThis项目将评估选择广泛使用的苹果砧木品种的C-螯合能力，通过分析三个关键组成部分：物理根测量：将使用生物化学方法来量化各种化合物（如总C，可溶性酚类物质，缩合单宁，酸不溶性馏分（木质素））根的抗氧化性进行评估。将使用根管、完整的岩心对根厚度、分解速率和根深度分布进行额外的测量（Dornbush等人，根分泌物生物化学分析：根分泌物沉积物的收集可以通过从盆栽砧木中收集蒸发（径流）水来进行（Leisso，et al.，2017），或如Gransee & Wittenmayer（2000）所述和评估的，通过将无菌生长的砧木的根浸入含有银（Ag+）的蒸馏水中。初级（糖、有机酸）和次级（酚类）代谢物将通过高效液相色谱法（HPLC）或气相色谱-质谱法（GC-MS）进行分析。微生物群落分析：高通量测序方法的最新进展极大地增强了微生物群落分析。将通过扩增通用16 S rRNA基因（细菌）和ITS序列（真菌）研究根际群落的细菌和真菌组成。特别是，该项目将重点关注与碳循环相关的功能群，如甲烷营养菌（甲烷生产者），产甲烷菌（甲烷消费者）和AMF。