Novel Method for Chromium Analysis in Biological Fluids

生物液体中铬分析的新方法

• 批准号: 6812050
• 负责人: ZILING (BEN) XUE
• 金额: $ 18.13万
• 依托单位: UNIVERSITY OF TENNESSEE KNOXVILLE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6812050
• 关键词: analytical chemistry; bioassay; biological signal transduction; body fluids; chemical binding; chromium; diabetes mellitus therapy; dietary mineral; dietary trace element; electrochemistry; electrodes; hydrogen peroxide; malnutrition; method development; noninsulin dependent diabetes mellitus; nutrient interaction; nutrition related tag; oxidation; ozone; ultraviolet radiation

DESCRIPTION (provided by applicant): There is a need to develop new reliable method for the analysis of chromium in biological fluids to assess chromium deficiency and the effect of chromium as adjuvant therapy for type 2 Diabetes. Chromium has been used as a low-cost dietary supplement for the treatment of diabetes and its complications. The frequency of actual chromium deficiency in the population is unknown in part because reliable measures for assessing chromium status in humans are limited. This R21 application seeks NIH support to develop a novel method in a two-year period to analyze chromium in biological fluids. The proposed novel method involves two steps. In the first step, the Advanced Oxidation Process (AOP) - a combination of hydrogen peroxide and ozone or ultraviolet (UV) radiation - is used to breakdown biological/organic species in biological fluids and to oxidize Iow-valent chromium to chromate. Chromate is then analyzed in the second step by a new membrane-based, chromate-selective electrode. This membrane containing a di-ammonium dicationic ligand preconcentrates chromate for its electrochemical analysis. The electrode is easily regenerated for subsequent analysis of chromium. The new method is expected to be easier and cheaper to operate for chromium analysis than the current techniques. The chemicals used in the novel method are environmentally benign, and do not generate harmful residuals or by-products. If chromium exists at different oxidation states, this novel method, converting chromium to chromate, gives the total chromium content in biological fluids. The research results can be applied immediately in the analysis of chromium in biological fluids, a goal articulated in the NIH program Chromium as Adjuvant Therapy for Type 2 Diabetes and Impaired Glucose Tolerance (PA-01-114).

描述（由申请人提供）：需要开发新的可靠方法来分析生物体液中的铬，以评估铬缺乏症和铬作为2型糖尿病辅助治疗的效果。铬已被用作治疗糖尿病及其并发症的低成本膳食补充剂。人群中实际铬缺乏症的频率尚不清楚，部分原因是评估人体铬状况的可靠措施有限。这项R21申请寻求NIH的支持，以在两年的时间内开发一种新的方法来分析生物液体中的铬。所提出的新方法包括两个步骤。在第一步中，高级氧化工艺（AOP）-过氧化氢和臭氧或紫外线（UV）辐射的组合-用于分解生物流体中的生物/有机物质并将低价铬氧化为铬酸盐。然后在第二步中通过新的基于膜的铬酸盐选择性电极分析铬酸盐。该膜含有二铵双阳离子配体，可预浓缩铬酸盐用于电化学分析。该电极易于再生，用于随后的铬分析。预计新方法比当前技术更容易、更便宜地进行铬分析。新方法中使用的化学品对环境无害，不会产生有害的残留物或副产品。如果铬以不同的氧化态存在，这种将铬转化为铬酸盐的新方法可以得出生物体液中的总铬含量。研究结果可以立即应用于生物液体中铬的分析，这是NIH项目铬作为2型糖尿病和糖耐量受损的辅助治疗（PA-01-114）中阐述的目标。