Synaptic processing in the gustatory brainstem
味觉脑干的突触处理
基本信息
- 批准号:6755552
- 负责人:
- 金额:$ 6.24万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-04-01 至 2004-09-30
- 项目状态:已结题
- 来源:
- 关键词:afferent nervebrain stemchemoreceptorschorda tympanicranial nerveselectrodeselectrophysiologyendogenous opioidhamstersmembrane potentialsneural information processingneural transmissionneuronsneuropeptide receptorneuropeptidesopioid receptorpotassium channelsingle cell analysissolitary tract nucleussynapsestastetaste budstissue /cell culturetonguevoltage /patch clamp
项目摘要
DESCRIPTION (provided by applicant): Inhibitory modulation is an important aspect of taste processing in the nucleus of the solitary tract (NST). Numerous in vivo and in vitro studies have demonstrated gamma-aminobutyric acid (GABA) to be an inhibitory neurotransmitter in taste processing in the NST. Recently, the influence of met-enkaphalin on taste responsive neurons in the rostral NST has been demonstrated in an in vivo model. In the present application the mechanisms through which opioids act as inhibitory neuropeptides in taste information processing in the gustatory NST are investigated. These experiments involve an in vitro brainstem slice preparation in which the activity of NST neurons that have been retrogradely labeled from the taste responsive region of the parabrachial nuclei (PbN) is recorded. These experiments test the hypothesis that both presynaptic and postsynaptic mechanisms are involved in opioid-mediated inhibition of taste activity.
Clinical studies show that patients with unilateral gustatory nerve damage often report no subjective change in taste experience. The mechanisms underlying this 'taste constancy' are thought to occur centrally, most likely in the NST. Several investigators have suggested that the loss of taste information from the chorda tympani (CT) nerve is compensated by a release of inhibition on IXth nerve input. However, the possibility of compensation by taste input from the other side of the tongue has never been investigated. The hypotheses in the present application are that NST neurons receive taste information from both the ipsilateral and contralateral sides of the tongue and that the ascending pathway from the contralateral side of the tongue involves glutamatergic projections from the contralateral NST. To address these hypotheses, the PI proposes to use both a whole-animal electrophysiological preparation, within which it is possible to investigate functionally how peripheral gustatory information is carried to the contralateral NST, and an in vitro slice preparation, in which the synaptic mechanisms involved in this bilateral communication can be studied in greater detail. These studies will provide new insights into the mechanisms by which neurons in the first central taste relay are involved in modulating and processing gustatory information.
描述(由申请人提供):抑制性调节是孤束核(NST)中味觉加工的一个重要方面。大量的体内和体外研究表明,γ-氨基丁酸(GABA)是一种抑制性神经递质在味觉加工的NST。最近,在体内模型中已经证明了甲硫恩卡法林对吻侧NST中味觉反应神经元的影响。在本申请中,阿片类药物作为味觉NST中味觉信息处理的抑制性神经肽的机制进行了研究。这些实验涉及在体外脑干切片制备,其中NST神经元,已从臂旁核(PbN)的味觉反应区逆行标记的活动被记录。这些实验验证了突触前和突触后机制都参与阿片类药物介导的味觉活性抑制的假设。
临床研究表明,单侧味觉神经损伤的患者通常报告味觉体验没有主观变化。这种“味觉恒定性”的机制被认为是集中发生的,最有可能发生在NST中。一些研究者认为,来自鼓索(CT)神经的味觉信息的损失通过对第九神经输入的抑制的释放来补偿。然而,从舌头的另一侧输入的味觉补偿的可能性从未被研究过。本申请中的假设是NST神经元接收来自舌头的同侧和对侧的味觉信息,并且来自舌头对侧的上行通路涉及来自对侧NST的味觉投射。为了解决这些假设,PI建议使用整个动物的电生理学准备,在其中,它是可能的功能研究如何外周味觉信息进行对侧NST,和体外切片制备,在这种双边通信中涉及的突触机制可以更详细地研究。这些研究将为第一中枢味觉中继神经元参与味觉信息调节和加工的机制提供新的见解。
项目成果
期刊论文数量(0)
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Cheng-Shu Li其他文献
Cheng-Shu Li的其他文献
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{{ truncateString('Cheng-Shu Li', 18)}}的其他基金
相似海外基金
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