Building novel functional fibres

构建新型功能纤维

• 批准号: 2294436
• 金额: --
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2294436
• 关键词: Building; novel; functional; fibres

The ability to design functions into materials is becoming a tangible goal. Functions such as electron transfer, conversion of energy (light to e-) or enzyme reactions would be possible to design and build into biologically produced fibrous material. The properties gained by such materials could have many applications in diagnostics, optics and biocatalysis.Many biological fibre forming systems are known based on carbohydrates and proteins e.g. bacterial cellulose or dextran and protein fibres such as collagen or poly-glutamate. In this PhD project we will use the properties of filamentous phages such as M13 which are long, 900 nm x 10 nm, naturally occurring molecules that self-assemble as they are secreted from E. coli. When M13 phage are concentrated they can further assemble together to form fibrous and liquid crystal domains. A cysteine containing phage will be used to build metal containing fibres and test these for electron transfer. We will use enzymes such as tyrosinase to functionalise surface tyrosines and transaminase to target lysine residues to enable cross-linking of phage with each other and with other filamentous proteins. Further functionalisation with enzymes arrayed on the surface will generate fibres with enzymic proerties that could be used in novel formats such as hybrid metal/enzyme catalysts.

将功能设计到材料中的能力正在成为一个切实的目标。诸如电子转移、能量转换（光到电子）或酶反应等功能将有可能被设计和构建到生物生产的纤维材料中。已知许多生物纤维形成系统基于碳水化合物和蛋白质（例如细菌纤维素或葡聚糖）和蛋白质纤维（例如胶原或聚谷氨酸）。在这个博士项目中，我们将利用丝状真菌的特性，如M13，它是长的，900 nm x 10 nm，天然存在的分子，自组装，因为它们是从E分泌。杆菌当M13噬菌体被浓缩时，它们可以进一步组装在一起以形成纤维状和液晶结构域。含半胱氨酸的噬菌体将用于构建含金属的纤维并测试这些纤维的电子转移。我们将使用酶如酪氨酸酶来功能化表面酪氨酸和转氨酶以靶向赖氨酸残基，以使噬菌体彼此交联并与其他丝状蛋白交联。用排列在表面上的酶进行进一步的功能化将产生具有酶特性的纤维，这些纤维可以用于新的形式，例如混合金属/酶催化剂。