Core-- Administration

体外模型和细胞培养核心是建立在所有项目的资源， PPG。具体目标是： 1.采集正常和CF组织。 2.人气道上皮细胞体外模型的建立。 3.体外模型和天然上皮细胞的表征。 a.电生理分析。 B.形态学评价。 C. CF细胞的基因分型。 4.研究和开发用于基因转移、气道上皮、细胞生物学和粘膜下腺体研究的新方法和模型。 5.为PPG的研究者生产培养细胞。 6.气道上皮细胞低温库的建立。 7.教授爱荷华州大学和其他机构的研究人员， 开发体外模型系统。 8.组织采集和细胞培养相关记录保存。 9.获得监管委员会的批准并保存细胞和动物的记录 问题研究 10.向其他机构的研究者提供细胞、质粒和病毒。 这个核心带来了一个高度积极性和技术先进的工作人员的专业知识， PPG研究者的受益。因此，它允许PPG调查人员专注于 在与CF基因转移高度相关的问题上拥有独特的专业知识。

The In Vitro Models and Cell Culture Core is established as a resource for all projects in the PPG. The specific aims are: 1. Acquisition of normal and CF tissue. 2. Generation of in vitro models of human airway epithelia. 3. Characterization of in vitro models and native epithelia. a. Electrophysiologic analysis. b. Morphologic evaluation. c. Genotyping of CF cells. 4. Research and development of new methods and models for the study of gene transfer, airway epithelial, cell biology, and submucosal glands. 5. Production of cultured cells for investigators in the PPG. 6. Development of a cryogenic bank of airway epithelial cells. 7. Teaching investigators at the University of Iowa and other institutions the methods for developing in vitro model systems. 8. Record keeping relevant to tissue acquisition and cell culture. 9. Obtaining approval from regulatory committees and record keeping for cell and animal studies. 10. Provision of cells, plasmids, and viruses to investigators at other institutions. This Core brings the expertise of a highly motivated and technically sophisticated staff to the benefit of the PPG investigators. As a result, it allows PPG investigators to focus their unique expertise on questions of high relevance to CF gene transfer.