Cell Culture Core

核心的结构使所有工作都由核心人员执行。个人调查员 希望使用细胞或其他材料或开发新模型的人与核心人员讨论所需的细胞类型、培养条件和实验或项目的总体目标。然后，核心人员将单元和模型提供给调查人员。通常，个别研究人员和核心人员一起工作，以提供特定类型研究所需的最佳条件(介质、播种密度、培养时间、底物、传代次数等)。新模型的开发通常是一个迭代过程，调查人员与核心人员交谈，核心人员开发模型，调查人员对模型进行评估并提出建议，核心人员试图改进模型，等等。 核心的组织有几个优点，a)它提供最高水平的质量 控制力。我们很少有感染的细胞系，表型的变化很早就被检测到了。B)它提供了对设施的最佳利用；c)通过让核心人员完成核心的所有工作，交通和时间冲突被降至最低；d)它为PPG调查人员提供了一支经验丰富的创新员工队伍，他们致力于开发模型，使调查人员能够对CF产生新的见解。 核心位于约550平方英尺的空间中，分别位于567、571和500B室 埃克斯坦医学研究大楼(EMRB)。EMRB包含所有4个项目的实验室(Davidson博士、威尔士博士、McCray博士和Zabner博士)。

The Core is structured so that all the work is performed by Core personnel. Individual investigators who wish to use cells or other materials or to develop new models discuss the type of cells needed, the culture conditions, and the overall goal of the experiment or project with Core personnel. Then, Core personnel provide the cells and models to the investigator. Frequently, the individual investigator and Core personnel work together to provide the optimal conditions (media, seeding density, duration of incubation, substrates, passage number, etc) required for a specific type of study. The development of new models is usually an iterative process, with investigators talking to the Core, Core personnel developing a model, the investigator evaluating it, and making suggestions, the Core personnel attempting to improve the model, and so on. The organization of the Core has several advantages, a) It provides the highest level of quality control. We rarely have infected cell lines and alterations in phenotype are detected eariy. b) It provides the best use of facilities, c) By having Core personnel do all the work of the Core, traffic and time conflicts are minimized, d) It provides PPG investigators with a highly experienced and innovative staff who are committed to developing models that will allow investigators to generate new insight into CF. The Core is located in approximately 550 square feet of space in rooms 567, 571, and 500B of the Eckstein Medical Research Building (EMRB). EMRB contains the laboratories of all 4 project Pis (Drs. Davidson, Welsh, McCray, and Zabner).