Cell Culture Core

核心的结构是，所有的工作都由核心人员执行。个体研究者 希望使用细胞或其他材料或开发新模型的人员与核心人员讨论所需细胞的类型、培养条件以及实验或项目的总体目标。然后，核心人员向研究者提供细胞和模型。通常，研究者和核心人员共同提供特定类型研究所需的最佳条件（培养基、接种密度、孵育持续时间、底物、传代次数等）。新模型的开发通常是一个迭代的过程，研究人员与核心人员交谈，核心人员开发模型，研究人员评估模型并提出建议，核心人员试图改进模型，等等。 核心的组织有几个优点，a）它提供了最高水平的质量 控制我们很少有感染的细胞系和表型的改变被发现早。B）它提供了设施的最佳利用，c）通过让核心人员完成核心的所有工作，最大限度地减少了交通和时间冲突，d）它为PPG调查人员提供了经验丰富和创新的工作人员，他们致力于开发模型，使调查人员能够对CF产生新的见解。 该核心位于约550平方英尺的空间，在房间567，571和500 B的 Eckstein Medical Research Building（EMRB）EMRB包含所有4个项目PI的实验室（Davidson、Welsh、McCray和Zabner博士）。

The Core is structured so that all the work is performed by Core personnel. Individual investigators who wish to use cells or other materials or to develop new models discuss the type of cells needed, the culture conditions, and the overall goal of the experiment or project with Core personnel. Then, Core personnel provide the cells and models to the investigator. Frequently, the individual investigator and Core personnel work together to provide the optimal conditions (media, seeding density, duration of incubation, substrates, passage number, etc) required for a specific type of study. The development of new models is usually an iterative process, with investigators talking to the Core, Core personnel developing a model, the investigator evaluating it, and making suggestions, the Core personnel attempting to improve the model, and so on. The organization of the Core has several advantages, a) It provides the highest level of quality control. We rarely have infected cell lines and alterations in phenotype are detected eariy. b) It provides the best use of facilities, c) By having Core personnel do all the work of the Core, traffic and time conflicts are minimized, d) It provides PPG investigators with a highly experienced and innovative staff who are committed to developing models that will allow investigators to generate new insight into CF. The Core is located in approximately 550 square feet of space in rooms 567, 571, and 500B of the Eckstein Medical Research Building (EMRB). EMRB contains the laboratories of all 4 project Pis (Drs. Davidson, Welsh, McCray, and Zabner).