In Vitro Models and Cell Culture Core

体外模型和细胞培养核心

• 批准号: 10248525
• 负责人: Joseph Zabner
• 金额: $ 18.54万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10248525
• 关键词: Biological Models; Cell Culture Techniques; Cell Line; Cells; Collaborations; Cultured Cells; Cystic Fibrosis; Data; Development; Electrophysiology (science); Epithelial; Epithelial Cells; Evaluation; Family suidae; Ferrets; Fibroblasts; Flow Cytometry; Functional disorder; Gene Expression; Generations; Goals; Health; Human; In Vitro; Institution; Iowa; Methodology; Methods; Mission; Modeling; Molecular; Morphology; Mutation; Phenotype; Process; Production; Pulmonary Cystic Fibrosis; Records; Research; Research Personnel; Resources; Services; Study models; Technical Expertise; Tissues; Universities; airway epithelium; biobank; bioelectricity; cell type; cystic fibrosis airway; experimental study; gene therapy; human model; human tissue; in vitro Model; investigator training; porcine model; tissue resource

PROJECT SUMMARY/ABSTRACT - In Vitro Models Cell Culture Core (CORE B ) The In Vitro Models and Cell Culture Core was established to provide well-differentiated epithelial models for studies on the pathophysiology of cystic fibrosis (CF) and for molecular and gene therapy experiments. The In Vitro Models and Cell Culture Core mission is to advance CF research to impact the health of people with CF. The Core achieves that mission by providing relevant models and technical expertise to investigators, developing new models and approaches, training investigators, and attracting new investigators to CF research and aiding their development. The goals of the In Vitro Models and Cell Culture Core are to collect, process and biobank tissue and airway epithelia from human, pig, and ferret. The Core also maintains a biobank of human non-CF and CF epithelia, including CF epithelia with rare mutations. The Core will also characterize models and native epithelia in collaboration with other Cores and investigators and will perform bioelectric analyses, morphological evaluation, gene expression profiles, and flow cytometry. Finally, the Core will teach investigators at the University of Iowa and other institutions how to successfully implement the methods for in vitro model systems.

项目摘要/摘要-体外模型细胞培养核心（Core B）