Role of E7 in HPV Infections

该提案寻求对我们研究生殖器人乳头瘤病毒（HPV）E7蛋白参与病毒感染发病机制的持续支持，在肛门生殖器癌中，高危病毒的E6和E7基因被选择性保留并表达，暗示其产物为癌蛋白。通过E6和E7永生化的上皮细胞在筏培养物中也表现出类似于在体内瘤形成中所见的分化能力改变。这些病毒癌蛋白永生化和改变上皮细胞分化能力的机制涉及与细胞周期调节相关的宿主蛋白的结合。E7蛋白结合视网膜母细胞瘤基因产物（Rb）以及相关的p107和p130蛋白。E7与Rb和p107的结合改变了E2F的调节 介导的转录在当前的资助期内，我们已经证明了E7在生产性病毒生命周期中的重要作用，通过促进病毒附加体的维持。此外，我们最近的研究已经确定E7和组蛋白脱乙酰酶（HDAC）之间的相互作用对于延长寿命和附加体维持是重要的。在这个更新的应用程序中，我建议调查的重要性，E7与Rb家族成员以及HDAC在病毒的生命周期中的相互作用，并剖析E7块细胞周期退出分化过程中的机制。我们将提出以下问题： 1）。在未分化和分化的细胞中，E7-Rb结合以及E7-HDAC相互作用对S期特异性启动子上的转录因子负载有什么影响？E7-HDAC复合物是否激活或抑制E2F诱导基因？ 2）。Rb、p130和p107在病毒生命周期的分化依赖性晚期有什么作用？通过siRNA废除Rb、p130和p107是否模拟E7对这些活性的影响。 3）。与Rb、p130、p107和HDAC的相互作用对低风险E7蛋白在病毒生命周期中的功能有什么影响？HPV 11 E7是否存在新的相互作用伴侣？

This proposal seeks continued support for our studies examining the mechanisms by which the E7 proteins of genital human papillomaviruses (HPV) contribute to the pathogenesis of viral infections, in anogenital cancers, the E6 and E7 genes of the high risk viruses are selectively retained and expressed implicating their products as oncoproteins. Epithelial cells immortalized by E6 and E7 also exhibit altered differentiation capabilities in raft cultures similar to those seen in neoplasias in vivo. The mechanisms by which these viral oncoproteins immortalize and alter the differentiation capabilities of epithelial cells involves the binding of host proteins involved in cell-cycle regulation. The E7 protein binds the retinoblastoma gene product (Rb) as well as the related p107 and p130 proteins. The binding of E7 to Rb and p107 alters the regulation of E2F mediated transcription. In the current funding period we have demonstrated an important role for E7 in the productive viral life cycle by acting to facilitate the maintenance of viral episomes. Furthermore, our recent studies have determined that the interaction between E7 and histone deacyelases (HDACs) is important for extension of life span and episomal maintenance. In this renewal application, I propose to investigate the importance of E7 interactions with Rb family members as well as HDACs in the viral life cycle and to dissect the mechanisms by which E7 blocks cell cycle exit during differentiation. We will ask the following questions: 1). What are the effects of E7-Rb binding as well as E7-HDAC interactions on the loading of transcription factors on S-phase specific promoters in undifferentiated and differentiated cells? Do the E7-HDAC complexes activate or repress E2F-inducible genes? 2). What are the effects of Rb, p130 and p107 during the differentiation dependent late phase of the viral life cycle? Does abrogation of Rb, p130 and p107 by siRNA mimic the effects of E7 on these activities. 3). What is the effect of interactions with Rb, p130 p107 and HDACs on the functions of low risk E7 protein in the viral life cycle? Are there any novel interacting partners of HPV 11 E7?