Array-Based Biological Sensor Using Nano-pores

描述(申请人提供)：免疫分析可能是疾病诊断和治疗中最常用的检测和量化生物分子的技术。在众多的免疫分析方法中，基于直接免疫分析的生物传感器因其具有快速检测、实时分析、传感装置可再生或重现性好、一次性廉价、无需前处理等优点而备受关注。另一方面，新兴的纳米技术通过在纳米尺度上控制物质并在同一水平上开发新的特性和现象来创造功能材料、设备和系统。 将纳米技术与免疫分析相结合，Lynntech正在提议开发一种基于阵列的生物传感器，用于检测血液、唾液和其他体液样本中的生物相关分子和物理目标，或用于研究实验室(纯化样本)、临床样本和活体。该传感器由具有机械稳定性的纳米孔径上的多个抗体组成，能够以最小的样本量同时监测多个蛋白质和分子，从而可以作为生物标志物使用。 第一阶段的工作将通过制造单个纳米孔、附着抗体、评估纳米孔传感器和制造纳米孔阵列来证明所提议的技术的可行性。向微型化生物标记物的技术的进一步发展将包括在第二阶段的工作中。

DESCRIPTION (provided by applicant): Immunoassay is probably the most commonly used technology for the detection and quantification of biomolecules in the diagnosis and management of disease. Among many immunoassay methodologies, biosensors based on direct immunoassay have been attractive because of their ideal advantages such as rapid detection, real-time analysis, regeneration of sensing device or reproducible inexpensive disposable, no pretreatment, and so on. On the other hand, emerging nanotechnology creates functional materials, devices, and systems through control of matter at nanomater scale and exploit novel properties and phenomena at the same level. Coupling the nanotechnology with the immunoassay, Lynntech is proposing development of an array-based biological sensor detecting biologically relevant molecular and physical targets in samples from blood, saliva and other body fluids, or for use in the research laboratory (purified samples), clinical specimens and in the living body. The sensor consists of multiple antibodies on nano-sized pores with mechanical stability to monitor multiple proteins and molecules with minimized sample volume simultaneously so that the sensor can be used as a biomarker. Phase I effort will demonstrate the feasibility of the proposed technology by fabricating a single nanopore, attaching antibodies, evaluating the nanopore sensor, and fabricating nanopore arrays. Further development of the technology toward miniaturized biomarker will be included in the Phase II effort.