Label-free Immunoassay-based Sensor Array for Assessment of Exposure to PAHs

用于评估 PAH 暴露的无标记免疫分析传感器阵列

• 批准号: 7495615
• 负责人: JINSEONG KIM
• 金额: $ 30万
• 依托单位: LYNNTECH, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-15 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7495615
• 关键词: Antibodies; Antibody Specificity; Antigen Targeting; Antigens; Area; Aromatic Hydrocarbons; Aromatic Polycyclic Hydrocarbons; Automation; Automobile Exhaust; Benzo(a)pyrene; Binding; Biological Markers; Biological Monitoring; Biological Products; Biosensor; Blood; Breathing; Businesses; Capital; Carbon; Characteristics; Chemical Agents; Chemicals; Clinical; Clinics and Hospitals; Complex; Condition; Consultations; Custom; DNA Adducts; Data; Data Analyses; Data Collection; Databases; Detection; Development; Development Plans; Devices; Diagnosis; Diagnostic; Disease Management; Disinfection; Drops; Engineering; Environment; Environmental Exposure; Environmental Health; Environmental Pollution; Equilibrium; Evaluation; Exposure to; Food; Genetic Programming; Goals; Government; Hand; Head; Human Genome; Immunoassay; Incubated; Individual; Industrial Waste; Industry; Ingestion; Institutes; Insurance; Intellectual Property; Label; Laboratories; Left; Leukocytes; Liquid substance; Marketing; Measurable; Measurement; Measures; Mechanics; Medical; Membrane; Methodology; Methods; Microprocessor; Miniaturization; Molecular; Monitor; Nanotechnology; Numbers; Occupational Health; Patients; Performance; Persons; Phase; Polycyclic Hydrocarbons; Population Study; Preparation; Process; Property; Proteins; Purpose; Range; Reporting; Research Institute; Resources; Risk Assessment; Route; Sales; Sampling; Science; Science of genetics; Sensitivity and Specificity; Serum; Signal Transduction; Spectrum Analysis; System; Techniques; Technology; Telemetry; Texas; Time; Translating; United States Environmental Protection Agency; Universities; Urine; Waste Products; Weight; Work; adduct; antigen antibody binding; base; cigarette smoking; commercial application; computer program; concept; cost; cross reactivity; design; electric impedance; environmental chemical; innovation; instrument; instrumentation; interest; member; nanopore; nanoscale; novel; point of care; pollutant; portability; professor; prototype; sensor; size; tool; trend; uptake; user-friendly

DESCRIPTION (provided by applicant) Polycyclic aromatic hydrocarbons (PAHs) constitute a complex group formed from incomplete combustion of organic carbon and are found in significant amounts in automobile exhaust, cigarette smoke, various foods, and industrial waste by-products. They represent serious, ubiquitously environmental contaminants. PAHs contamination of individuals occurs by inhalation, ingestion, or contact. Some PAHs are carcinogenic, teratogenic, and mutagenic. Biomonitoring is essential to make precise, quantitative measurements of personal exposure to environmental chemical/biological agents because it can provide an assessment of the integrated uptake through all exposure routes. Conventional biomonitoring methods are often complex, time-consuming, labor intensive, and expensive. Immunoassay is probably the most commonly used technology for the detection and quantification of biomolecules in the diagnosis and management of disease. Current trends in the development of immunoassay methodologies are miniaturization, multiple detection, and automation. On the other hand, nanotechnology has recently boomed because of the availability of new investigative tools. This technology makes it possible to characterize chemical and mechanical properties at a single molecular level, discover novel phenomena and processes, and provide science with a wide range of tools, materials, devices, and systems with unique characteristics. The aim of this project is to develop a novel multiple biological sensor combining immunoassay (antibodybased detection) and emerging nanotechnology (a nanoscale biosensor) to assess real time and continuous readout of internal exposure to priority environmental chemical agents, specifically to PAHs. In this innovative approach, the presence of targeted biomolecules in serum is monitored by specific antigen-antibody interaction at the nanoscale. Specific antigen-antibody interactions make this approach highly precise to identify specific antigens, such as biomarkers for PAH exposure, enabling accurate assessment of the exposure. Other advantages of this approach over conventional immunoassay techniques are (i) no other signal-generating moieties (labeling agents) are needed to measure the antigen-antibody interaction, (ii) multiplex assessment of informative and predictive serum biomarkers is possible, and (iii) wide range of applications. During the Phase I project, the proof-of-concept will be established, including the detection of antibody/antigen interactions in serum and in the presence of other biomarkers of other environmental exposure set. The proposed system has a high potential for user acceptance and has immediate commercial applications in many areas. Key segments in the medical diagnostic markets where the proposed technology can have an immediate impact include hospitals, clinics, clinical laboratories, medical insurance companies, and point-of-care uses.

描述（由申请人提供） 多环芳烃（PAHs）是由有机碳不完全燃烧形成的复杂基团，在汽车尾气、香烟烟雾、各种食品和工业废物副产品中大量存在。它们是严重的、无处不在的环境污染物。多环芳烃污染的个人发生吸入，摄入或接触。一些多环芳烃具有致癌、致畸和致突变性。生物监测对于精确、定量测量个人接触环境化学/生物制剂的情况至关重要，因为它可以评估通过所有接触途径的综合吸收情况。传统的生物监测方法通常是复杂的，耗时的，劳动密集型的，昂贵的。 免疫分析可能是疾病诊断和管理中最常用的生物分子检测和定量技术。目前免疫分析方法学的发展趋势是微型化、多重检测和自动化。另一方面，由于新的调查工具的可用性，纳米技术最近蓬勃发展。该技术可以在单个分子水平上表征化学和机械性能，发现新的现象和过程，并为科学提供具有独特特性的各种工具，材料，设备和系统。 该项目的目的是开发一种新型的多生物传感器，结合免疫测定（基于抗体的检测）和新兴的纳米技术（纳米级生物传感器），以评估真实的时间和连续读出的内部暴露于优先环境化学剂，特别是多环芳烃。在这种创新的方法中，通过纳米级的特异性抗原-抗体相互作用来监测血清中靶向生物分子的存在。特异性抗原-抗体相互作用使这种方法高度精确地识别特定抗原，例如PAH暴露的生物标志物，从而能够准确评估暴露。这种方法相对于常规免疫测定技术的其他优点是（i）不需要其他信号产生部分（标记剂）来测量抗原-抗体相互作用，（ii）可以进行信息性和预测性血清生物标志物的多重评估，以及（iii）广泛的应用。在I期项目期间，将建立概念验证，包括检测血清中的抗体/抗原相互作用以及存在其他环境暴露集的其他生物标志物的情况。 建议的系统具有很高的潜力，用户接受，并立即在许多领域的商业应用。医疗诊断市场的关键部分，其中拟议的技术可以产生直接影响，包括医院，诊所，临床实验室，医疗保险公司和护理点用途。