Array-Based Biological Sensor Using Nano-pores

使用纳米孔的基于阵列的生物传感器

• 批准号: 6689836
• 负责人: JINSEONG KIM
• 金额: $ 26.41万
• 依托单位: LYNNTECH, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6689836
• 关键词: biomarker; biosensor device; biotechnology; clinical chemistry; immunoconjugates; immunologic assay /test; nanotechnology; technology /technique development

DESCRIPTION (provided by applicant): Immunoassay is probably the most commonly used technology for the detection and quantification of biomolecules in the diagnosis and management of disease. Among many immunoassay methodologies, biosensors based on direct immunoassay have been attractive because of their ideal advantages such as rapid detection, real-time analysis, regeneration of sensing device or reproducible inexpensive disposable, no pretreatment, and so on. On the other hand, emerging nanotechnology creates functional materials, devices, and systems through control of matter at nanomater scale and exploit novel properties and phenomena at the same level. Coupling the nanotechnology with the immunoassay, Lynntech is proposing development of an array-based biological sensor detecting biologically relevant molecular and physical targets in samples from blood, saliva and other body fluids, or for use in the research laboratory (purified samples), clinical specimens and in the living body. The sensor consists of multiple antibodies on nano-sized pores with mechanical stability to monitor multiple proteins and molecules with minimized sample volume simultaneously so that the sensor can be used as a biomarker. Phase I effort will demonstrate the feasibility of the proposed technology by fabricating a single nanopore, attaching antibodies, evaluating the nanopore sensor, and fabricating nanopore arrays. Further development of the technology toward miniaturized biomarker will be included in the Phase II effort.

描述（由申请人提供）：免疫分析可能是疾病诊断和管理中最常用的生物分子检测和定量技术。在众多的免疫分析方法中，基于直接免疫分析法的生物传感器因其具有快速检测、实时分析、传感装置可再生或可重复、廉价一次性、无需预处理等理想优势而备受关注。另一方面，新兴的纳米技术通过在纳米尺度上控制物质来创造功能材料、设备和系统，并在同一水平上开发新的特性和现象。