LC-MS/MS for Biomedical Research

描述（由申请人提供）：申请资金用于购买液相色谱仪-三重四极杆-质谱仪（LC-TQ MS） Waters Quattro微型API串联四极杆系统，作为威斯康星医学院研究人员的共享仪器。该学院有950名教师和NIH资助，但没有共享LC-MS/MS仪器。该仪器对未来的研究至关重要。在过去的11年里，药理学和毒理学系的质谱共享设施为学院的教师提供了质谱专业知识和仪器。该设施制定了处理来自多个用户的样品和处理具有不同化学特性的样品的规程。它有一台惠普气相色谱仪-单四极杆质谱仪（引擎）和一台安捷伦液相色谱仪-单四极杆质谱仪（LC-MSD）。LC-MSD目前被许多研究人员广泛用于脂质，类固醇激素，肽和蛋白质的分析。这些不同的需求创造了对LC-MS分析的不断增长的需求。因此，我们要求购买LC-TQ质谱仪，因为它的质谱/质谱功能将克服当前仪器的许多限制。这种质谱联用能力将有助于未知化合物的鉴定。该功能目前由GC- ms执行，需要样品衍生并与GC条件兼容。LC-TQ质谱也有助于测量具有相同m/z的分析物，因为可以选择不同m/z的子离子进行测量。这将增加对复杂样品中某些峰分析的置信度。这也将缩短运行时间，因为LC-MSD需要具有相同m/z的多种化合物的完整分辨率。这项拨款的15个主要用户的需求分为四类：(1)类二十烷的鉴定和测量，(2)脂质过氧化产物、异前列腺素和氧脂质的测量，(3)成瘾性药物及其代谢物的分析，以及(4)其他应用。大约75-80%的仪器时间将提供给这些参与者，其余时间将提供给学院的其他研究人员。

DESCRIPTION (provided by applicant): Funds are requested to purchase a liquid chromatograph - triple quadrupole - mass spectrometer (LC-TQ MS) Waters Quattro micro API Tandem Quadrupole System) for use as a shared instrument by researchers of the Medical College of Wisconsin. The College has 950 faculty and NIH funding but has no shared LC-MS/MS instrumentation. This instrument is critical for future research. During the past 11 years, the Mass Spectrometry Shared Facility of the Department of Pharmacology and Toxicology has provided mass spectrometry expertise and instrumentation to the faculty of the College. The Facility has established protocols for handling samples from multiple users and dealing with samples with different chemical characteristics. It has a Hewlett - Packard gas chromatograph - single quadrupole mass spectrometer (Engine) and an Agilent liquid chromatograph - single quadrupole mass spectrometer (LC-MSD). The LC-MSD is currently widely used by a number of investigators for analysis of lipids, steroid hormones, peptides and proteins. These varied needs have created an increasing demand for LC-MS analysis. Therefore, we request the purchase of a LC-TQ MS because of its MS/MS capabilities that will overcome many of the limitations of the current instrument. This MS/MS capability will facilitate the identification of unknown compounds. This function is currently performed by GC-MS, which requires sample derivatization and compatibility with GC conditions. The LC-TQ MS will also facilitate the measurement of analytes with the same m/z since daughter ions of different m/z may be selected for measurement. This will result in increase in confidence in analysis of certain peaks in complex samples. It will also result in shorter run time since the LC-MSD needs a complete resolution of multiple compounds with same m/z. The needs of the 15 principal users of this grant fall into four categories: (1) identification and measurement of eicosanoids, (2) measurement of lipid peroxidation products, isoprostanes and oxylipids, (3) analysis of addictive drugs and their metabolites, and (4) miscellaneous applications. About 75-80% of instrument time will be available to these participants and the remaining time will be available to other investigators at the College.