High-resolution liquid chromatography mass spectrometry system

高分辨率液相色谱质谱系统

• 批准号: 524805621
• 金额: --
• 依托单位: Johannes Gutenberg-Universität Mainz
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/524805621?language=en
• 关键词: resolution; liquid; chromatography; mass; spectrometry

The Institute of Molecular Biology (IMB) is a basic research institute in the field of epigenetics, genome stability, and aging. IMB is equipped with state-of-the-art core facilities, which offer services to the entire scientific community in Mainz. The Proteomics Core Facility (PCF) operates a high-resolution mass spectrometer coupled to a nanoflow UHPLC system. The PCF offers routine services that range from simple gel band identification to quantitative analysis of complex samples. Currently, it supports labelfree, dimethyl-labelled, TMT and SILAC based quantitative proteomic experiments. If further deep proteome analysis is desired, PCF can provide multiple options of off-line protein/peptide fractionation. It also supports SILAC experiments by providing in-house certified reagents and testing incorporation efficiency of the metabolic labelling. In addition, PCF is available to users for consultation on experimental design, troubleshooting and bioinformatics data analysis. The PCF staff consist of both wet- and drylab scientists that possess a broad range of research experience and technical expertise. In order to be able to continue to offer advanced equipment, the current 10-year-old mass spectrometer would have to be upgraded to a new liquid chromatography mass spectrometry system that could cover all the above-mentioned areas of application.

分子生物学研究所（IMB）是表观遗传学，基因组稳定性和衰老领域的基础研究所。IMB配备了最先进的核心设施，为美因茨的整个科学界提供服务。蛋白质组学核心设施（PCF）操作一个高分辨率质谱仪耦合到一个nanoflow UHPLC系统。PCF提供从简单的凝胶带鉴定到复杂样品定量分析的常规服务。目前，它支持无标记，二甲基标记，TMT和SILAC为基础的定量蛋白质组学实验。如果需要进一步深入的蛋白质组分析，PCF可以提供多种离线蛋白质/肽分级分离的选择。它还通过提供内部认证的试剂和测试代谢标记的掺入效率来支持SILAC实验。此外，PCF可供用户咨询实验设计，故障排除和生物信息学数据分析。PCF的工作人员包括湿实验室和干实验室的科学家，他们拥有广泛的研究经验和技术专长。为了能够继续提供先进的设备，目前已有10年历史的质谱仪必须升级为新的液相色谱质谱系统，以覆盖上述所有应用领域。