Liquid chromatography-mass spectrometry system for metabolite-based precision medicine

基于代谢物的精准医学液相色谱-质谱系统

• 批准号: MR/X013758/1
• 负责人: Warwick Dunn
• 金额: $ 58.85万
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FX013758%2F1
• 关键词: Liquid; chromatography; mass; spectrometry; system

Metabolites are small biochemicals present in every human cell, tissue and biofluid. These metabolites are essential for life, are primarily involved in metabolism and include sugars and fats which are important for the generation of energy through metabolism of food. Metabolites have other important roles which help us live including the construction of other biochemicals (proteins and DNA), construction of cell walls with fats and the control of chemical processes in the body in response to environmental changes including eating and exercise. The amounts of metabolites in our bodies change in response to environmental changes or in relation to the development and progression of diseases. As examples, the metabolite lactic acid quickly increases in amounts in muscle tissue and blood during exercise and the metabolite glucose increases in blood in the disease diabetes. In scientific research we study metabolites to understand how we age and how disease processes start and progress. This research can help us understand how diseases develop, help us identify new treatments for diseases and help us diagnose a disease or define the risk of developing a disease early which reduces the level and intensity of treatment and increases the probability of long term survival. The research to be performed will apply the study of thousands of metabolites and this scientific technique is called metabolomics. We will apply a scientific instrument called liquid chromatography-mass spectrometry to allow detection of metabolites based on their weight as different metabolites have different weights (masses). Metabolomics allows the study of thousands of metabolites in discovery research, identification of a small number of important metabolites related to the clinical question and translation in to clinical practice. Metabolites are already used for disease diagnosis including glucose for diagnosis of diabetes. Metabolomics will be applied to study a range of diseases including cancers (lung, blood colorectal), cardiovascular diseases, hormone-driven diseases and women's health/pregnancy disorders.Importantly, not all humans are the same in how diseases develop and response to different treatments. Traditional medicine treated everyone as if they were the same. A new era of precision medicine is upon us and is an innovative approach to tailoring disease prevention and treatment that takes into account differences in people's genes, environments, and lifestyles. The goal of precision medicine is to target the right treatments to the right patients at the right time. The studies performed will help develop new precision medicine tools for early disease diagnosis or for development of different treatments.

代谢物是存在于每个人体细胞、组织和生物体液中的小生物化学物质。这些代谢物是生命所必需的，主要参与新陈代谢，包括糖和脂肪，它们对通过食物代谢产生能量很重要。代谢物还有其他重要的作用，包括其他生物化学物质（蛋白质和DNA）的构建，脂肪细胞壁的构建以及控制体内化学过程以应对环境变化，包括饮食和运动。我们体内代谢物的数量会随着环境的变化或疾病的发展而变化。例如，运动时肌肉组织和血液中的代谢物乳酸迅速增加，糖尿病患者血液中的代谢物葡萄糖增加。在科学研究中，我们通过研究代谢物来了解我们是如何衰老的，以及疾病是如何开始和发展的。这项研究可以帮助我们了解疾病是如何发展的，帮助我们确定疾病的新治疗方法，帮助我们诊断疾病或确定早期发展疾病的风险，从而降低治疗水平和强度，增加长期生存的可能性。即将进行的研究将应用于对数千种代谢物的研究，这种科学技术被称为代谢组学。由于不同的代谢物有不同的重量（质量），我们将使用一种叫做液相色谱-质谱法的科学仪器来检测代谢物的重量。代谢组学允许在发现研究中对数千种代谢物进行研究，识别少量与临床问题相关的重要代谢物并将其转化为临床实践。代谢物已经被用于疾病诊断，包括用于诊断糖尿病的葡萄糖。代谢组学将被应用于研究一系列疾病，包括癌症（肺癌、血结直肠癌）、心血管疾病、激素驱动疾病和妇女健康/妊娠障碍。重要的是，并不是所有的人在疾病的发展和对不同治疗的反应方面都是一样的。传统医学对待每个人都是一样的。精准医疗的新时代即将到来，这是一种创新的方法，可以根据人们的基因、环境和生活方式的差异来定制疾病的预防和治疗。精准医疗的目标是在正确的时间为正确的病人提供正确的治疗。这些研究将有助于开发新的精准医疗工具，用于早期疾病诊断或开发不同的治疗方法。

项目成果

A proposed framework to evaluate the quality and reliability of targeted metabolomics assays from the UK Consortium on Metabolic Phenotyping (MAP/UK)

• DOI: 10.1038/s41596-022-00801-8
• 发表时间: 2023-02-24
• 期刊: NATURE PROTOCOLS
• 影响因子: 14.8
• 作者: Sarmad，Sarir;Viant，Mark R.;Suzuki，Toru
• 通讯作者: Suzuki，Toru