MRI: Acquisition of a Liquid Chromatography - Mass Spectrometry System

MRI：获取液相色谱-质谱系统

• 批准号: 2215307
• 负责人: Richard Biagioni
• 金额: $ 38.35万
• 依托单位: Missouri State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2215307&HistoricalAwards=false
• 关键词: MRI; Acquisition; Liquid; Chromatography; Mass

This award is jointly supported by the Major Research Instrumentation and the Chemistry Research Instrumentation Programs. Missouri State University is acquiring a liquid chromatography mass spectrometer (LC/MS) to support the research of Professor Richard Biagioni and colleagues Laszlo Kovacs, Cyren Rico, Natasha DeVore, and Paul Durham. This instrument facilitates research in the areas of analytical chemistry, biochemistry, environmental chemistry, and biology. Mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with a liquid chromatograph provides additional structural identification power by separating mixtures of compounds before they reach the mass spectrometer. This instrument enhances the educational, research, and teaching efforts of students at all levels in many departments as well as provides accessibility for use at nearby institutions. Students gain hands-on experience with the instrument in their research and learning in diverse fields including analytical chemistry, biochemistry, environmental chemistry, and biology.The award of the LC/MS is aimed at enhancing research and education at all levels, especially of benefit to the scientific community in areas such as animal and plant metabolism; disease and treatments; and studies relevant to agriculture. The instrument enables research focused on the metabolomics of plants exposed to pollutants, the analysis of flavonoids in grapes as a function of rootstock, the effects of grape seed extracts on trigeminal pain signaling, and dietary supplements as prebiotics to suppress migraine and TMD pain. It also assists in the study of metabolites related to orphan human CYP4X1and canine cytochrome P450, in elucidating the molecular basis of alpha-synuclein fibrillation and other amyloid proteins, and in the development of peptide- and peptidomimetic-based therapeutics that capture and neutralize melittin and histones. Other investigations include quantifying the presence of modified DNA bases that lead directly to deleterious effects within the cell, metalloporphyrins that contain lanthanide metal centers as novel ionophores, ultraviolet light exposure in calcium regulation of a benthic reptile, and the activity and reproductive patterns of the critically endangered Central American River Turtle.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由主要研究仪器和化学研究仪器计划共同支持。密苏里州州立大学正在购买一台液相色谱质谱仪（LC/MS），以支持Richard Biagioni教授及其同事Laszlo Kovacs、Cyren Rico、Natasha DeVore和Paul达勒姆的研究。该仪器有助于分析化学、生物化学、环境化学和生物学领域的研究。 质谱（MS）是用于识别和表征嵌入复杂基质中的少量化学物质的关键分析方法之一。在典型的实验中，组分流入质谱仪，在那里它们被电离成母离子及其碎片离子，并测量它们的质量。这种高灵敏度的技术可以检测和确定复杂混合物中分子的结构。带有液相色谱仪的仪器通过在化合物到达质谱仪之前分离化合物的混合物来提供额外的结构鉴定能力。该工具增强了许多部门各级学生的教育，研究和教学努力，并提供了在附近机构使用的无障碍环境。学生们可以在分析化学、生物化学、环境化学和生物学等不同领域的研究和学习中获得该仪器的实践经验。LC/MS奖旨在加强各级研究和教育，特别是在动植物代谢、疾病和治疗以及与农业相关的研究等领域对科学界有益。 该仪器使研究集中在暴露于污染物的植物的代谢组学，葡萄中黄酮类化合物的分析作为砧木的功能，葡萄籽提取物对三叉神经疼痛信号的影响，以及膳食补充剂作为益生元抑制偏头痛和TMD疼痛。它还有助于研究与孤儿人CYP 4X 1和犬细胞色素P450相关的代谢物，阐明α-突触核蛋白纤维化和其他淀粉样蛋白的分子基础，以及开发捕获和中和蜂毒肽和组蛋白的基于肽和肽模拟物的治疗方法。其他研究包括量化修饰的DNA碱基的存在，这些碱基直接导致细胞内的有害作用，含有镧系金属中心作为新型离子载体的金属卟啉，底栖爬行动物钙调节中的紫外线暴露，以及极度濒危的中美洲河龟的活动和繁殖模式。该奖项反映了NSF的法定使命，并被认为是值得支持的，使用基金会的知识价值和更广泛的影响审查标准进行评估。