MRI: Acquisition of a Liquid Chromatography - Mass Spectrometry System

该奖项由主要研究仪器项目和化学研究仪器项目共同支持。密苏里州立大学正在购买一台液体色谱质谱计(LC/MS)，以支持理查德·比亚戈尼教授及其同事Laszlo Kovacs、Cyren Rico、Natasha DeVore和Paul Durham的研究。该仪器促进了分析化学、生物化学、环境化学和生物学领域的研究。质谱仪(MS)是鉴定和表征镶嵌在复杂基质中的少量化学物种的关键分析方法之一。在一个典型的实验中，这些组分流入质谱仪，在那里它们被电离成母离子，并测量它的碎片离子及其质量。这种高度灵敏的技术可以检测和确定复杂混合物中的分子结构。带有液体色谱仪的仪器通过在混合物到达质谱计之前将其分离来提供额外的结构鉴定能力。这一工具加强了许多部门各级学生的教育、研究和教学努力，并提供了在附近机构使用的便利条件。学生在分析化学、生物化学、环境化学和生物学等不同领域的研究和学习中获得实践经验。LC/MS获奖的目的是加强各个层面的研究和教育，特别是在动植物新陈代谢、疾病和治疗以及与农业有关的研究等领域对科学界有利。该仪器使研究重点放在植物暴露在污染物中的代谢组学，分析葡萄中的类黄酮类化合物作为砧木的功能，葡萄籽提取物对三叉神经疼痛信号的影响，以及作为益生菌的膳食补充剂抑制偏头痛和TMD疼痛。它还帮助研究与孤儿人类细胞色素P4X1和犬细胞色素P450相关的代谢物，阐明α-突触核蛋白纤颤和其他淀粉样蛋白的分子基础，以及开发捕获和中和蜂毒素和组蛋白的基于多肽和仿肽的疗法。其他调查包括量化直接导致细胞内有害影响的修饰DNA碱基的存在，将稀土金属中心作为新的离子载体的金属卟啉，底栖爬行动物钙调节中的紫外线暴露，以及极度濒危的中美洲河龟的活动和繁殖模式。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

This award is jointly supported by the Major Research Instrumentation and the Chemistry Research Instrumentation Programs. Missouri State University is acquiring a liquid chromatography mass spectrometer (LC/MS) to support the research of Professor Richard Biagioni and colleagues Laszlo Kovacs, Cyren Rico, Natasha DeVore, and Paul Durham. This instrument facilitates research in the areas of analytical chemistry, biochemistry, environmental chemistry, and biology. Mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with a liquid chromatograph provides additional structural identification power by separating mixtures of compounds before they reach the mass spectrometer. This instrument enhances the educational, research, and teaching efforts of students at all levels in many departments as well as provides accessibility for use at nearby institutions. Students gain hands-on experience with the instrument in their research and learning in diverse fields including analytical chemistry, biochemistry, environmental chemistry, and biology.The award of the LC/MS is aimed at enhancing research and education at all levels, especially of benefit to the scientific community in areas such as animal and plant metabolism; disease and treatments; and studies relevant to agriculture. The instrument enables research focused on the metabolomics of plants exposed to pollutants, the analysis of flavonoids in grapes as a function of rootstock, the effects of grape seed extracts on trigeminal pain signaling, and dietary supplements as prebiotics to suppress migraine and TMD pain. It also assists in the study of metabolites related to orphan human CYP4X1and canine cytochrome P450, in elucidating the molecular basis of alpha-synuclein fibrillation and other amyloid proteins, and in the development of peptide- and peptidomimetic-based therapeutics that capture and neutralize melittin and histones. Other investigations include quantifying the presence of modified DNA bases that lead directly to deleterious effects within the cell, metalloporphyrins that contain lanthanide metal centers as novel ionophores, ultraviolet light exposure in calcium regulation of a benthic reptile, and the activity and reproductive patterns of the critically endangered Central American River Turtle.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.