Parallel Production of Molecules for Structural Biology

结构生物学分子的并行生产

• 批准号: 6732567
• 负责人: ALAN M FRIEDMAN
• 金额: $ 49.98万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6732567
• 关键词: bioimaging /biomedical imaging; biomedical automation; biomedical equipment purchase; chromatography; crystallization; expression cloning; macromolecule; photography; robotics; structural biology

DESCRIPTION (provided by applicant): Three-dimensional structures of biological macromolecules have a huge impact on our understanding of biology at the molecular level. Increasingly, the limiting factor in obtaining structural information is the production of materials suitable for structural study. This means sufficient quantities of pure, stable, active macromolecules, their fragments or complexes. High-throughput technologies that were developed for large projects of genome sequencing, structural genomics and proteomics also can be applied to classic, problem based structural biology. A group of investigators at Purdue University whose research focuses on structural biology wish to adopt these new technologies for parallel, small-scale production of macromolecules. All of the investigators have projects that would benefit enormously from broad sampling of biological sources, expression constructs, expression conditions and purification protocols for the target molecules. This proposal seeks instruments for parallel cloning, expression, purification and crystallization. Use of these instruments would fundamentally alter the way in which the investigators approach their research problems and would greatly enhance their productivity. The requested instruments are: a liquid-handling robot for cloning and expression, an automated colony picker, an automated chromatography system, and a crystallization robot with photographic monitoring system.

描述(由申请人提供)： 生物大分子的三维结构对我们从分子水平上理解生物学有着巨大的影响。越来越多地，获取结构信息的限制因素是生产适合于结构研究的材料。这意味着有足够数量的纯的、稳定的、活性的大分子、它们的碎片或复合体。为基因组测序、结构基因组学和蛋白质组学等大型项目开发的高通量技术也可以应用于经典的、基于问题的结构生物学。普渡大学的一组研究人员专注于结构生物学，他们希望采用这些新技术来并行、小规模地生产大分子。所有研究人员的项目都将从对目标分子的生物来源、表达结构、表达条件和纯化方案的广泛采样中受益匪浅。这项提议寻求平行克隆、表达、纯化和结晶的工具。使用这些工具将从根本上改变调查人员处理研究问题的方式，并将极大地提高他们的生产率。所要求的仪器是：一个用于克隆和表达的液体处理机器人，一个自动菌落拾取器，一个自动层析系统，以及一个带有摄影监控系统的结晶机器人。