CRYSTALLIZATION OF PROTEIN ISOASPARTYL METHYLTRANSFERASE

蛋白质异天冬氨酰甲基转移酶的结晶

• 批准号: 2407705
• 负责人: ALAN M FRIEDMAN
• 金额: $ 7.18万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-15 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2407705
• 关键词: Archaea; X ray crystallography; animal genetic material tag; aspartate; crystallization; enzyme activity; high performance liquid chromatography; isozymes; methyltransferase; molecular cloning

This proposal is for pilot studies leading to crystallization and structure determination by x-ray diffraction of protein isoaspartyl methyltransferase (PIMT. PIMT catalyzes a process for reparing proteins which have been damaged by a specific spontanious mechanism. This mechanism converts the normal asparagine residues of proteins into either aspartic acid residues or abnormal isoaspartyl residues. By transferring a methyl group to the abnormal residue, PIMT initiates a repair pathway for these damaged proteins. The importance of this pathway is suggested by its prevalence and conservation throughout the bioligical world. Proteins damaged by isoaspartyl formation accumulate with age and have been associated with several disease conditions. Structure determination of the PIMT enzyme will enhance our understanding of this process and perhaps allow us to design better enzymes for diagnostic and therapeutic purposes.

本建议书是用于导致结晶和结构的中试研究 x射线衍射法测定蛋白质中的异戊酰 甲基转移酶（PIMT. PIMT催化修复过程 蛋白质被一种特殊的自发性 机制 这种机制将正常的天冬酰胺残基 蛋白质的天冬氨酸残基或异常的异戊二酰 残基 通过将甲基转移到异常残基上， PIMT启动这些受损蛋白的修复途径。 的 这一途径的重要性是由它的普遍性和 在整个生物世界中进行保护。 蛋白质受损 异戊酰基形成随着年龄的增长而积累 患有多种疾病 PIMT的结构测定 酶将增强我们对这一过程的理解， 使我们能够设计出更好的酶用于诊断和治疗 目的

项目成果

Crystal structure of protein isoaspartyl methyltransferase: a catalyst for protein repair.

• DOI: 10.1016/s0969-2126(00)00522-0
• 发表时间: 2000-11
• 期刊: Structure
• 影响因子: 5.7
• 作者: Matthew M. Skinner;J. M. Puvathingal;Rick Walter;Alan M. Friedman