Ultra high field 900 MHz NMR spectrometer

描述（由申请人提供）：本申请申请为克利夫兰结构生物学中心（CCSB）购买一台超高场900 MHz核磁共振（NMR）光谱仪，该中心由凯斯西储大学、Lemer研究所和克利夫兰州立大学组成。已经从几个机构获得了很大一部分资金，但来自NIH的额外支持对于确保世界级核磁共振光谱仪的全面运行至关重要。该仪器将使CCSB的结构生物学家以及俄亥俄州其他机构的结构生物学家能够推动核磁共振技术的前沿，并将他们的详细结构研究扩展到蛋白质和蛋白质复合物，这些蛋白质和蛋白质复合物的因子比现有低场核磁共振仪器目前施加的25 kDa限制大2到5倍。本提案的主要用户都有资金充足的研究项目（95%由NIH资助），并积极参与生物分子核磁共振波谱的开发和应用。新仪器的功能显著增强，将使他们能够使用尖端的核磁共振技术进行各种具有挑战性的结构研究，包括大型细胞信号蛋白复合物、蛋白质核酸识别、膜蛋白/受体、蛋白质折叠/错误折叠中间体和多亚基组装。事实上，所有主要的核磁共振研究人员，特别是CCSB的研究人员，最近都在前往其他城市和州，以获得超高场核磁共振仪器。该仪器的收购不仅将提供核磁共振光谱访问重要的新类大分子和复合物，而且还将吸引一流的教师，学生和博士后到结构生物学领域。新仪器将在培养研究生和博士后方面发挥直接作用。它不仅将对区域结构生物学学界的研究项目产生重大影响，而且还将造就一支由最先进技术训练的核磁共振结构生物学专家骨干队伍。反过来，这将增加该地区专业知识的临界质量，这将促进中西部尖端生物研究和相关高科技产业的发展。

DESCRIPTION (provided by applicant): This proposal requests funding for purchasing an ultra high field 900 MHz nuclear magnetic resonance (NMR) spectrometer for Cleveland Center for Structural Biology (CCSB), which is composed of Case Western Reserve University, Lemer Research Institute, and Cleveland State University. A significant portion of funding has been obtained from several agencies but additional support from NIH is essential to secure the full operation of the world-class NMR spectrometer. This instrument will enable the structural biologists in CCSB as well as those in other Ohio institutions to push the frontier of NMR technology and to extend their detailed structural studies to proteins and protein complexes that are factors of two to five larger than the present 25 kDa limit imposed by the existing lower field NMR instruments. Major users in this proposal all have well funded research programs (95 percent NIH funded) and are actively involved in the development and application of biomolecular NMR spectroscopy. The dramatically enhanced capabilities of the new instrument will allow them to use cutting-edge NMR technology to undertake a variety of challenging structural studies involving large cellular signaling protein complexes, protein-nucleic acid recognition, membrane proteins/receptors, protein folding/misfolding intermediates, and multi-subunit assemblies. Virtually all of the major NMR investigators, especially those in CCSB, have recently been traveling to other cities and states to gain access to ultrahigh field NMR instrumentation. Acquisition of this instrument will not only afford NMR spectroscopic access to important new classes of macromolecules and complexes but also attract top-notch faculty, students and post-docs to the area of structural biology. The new instrument will play a direct role in training graduate students and postdoctoral fellows. It will not only make significant impact on research projects of regional structural biology community but also create a cadre of NMR structural biology experts trained by the most advanced techniques. In turn, this will increase the critical mass of expertise in the region, which will promote the development of cutting edge biological research and associated high technology industry in the mid-west.