LCQ Deca XP Ion Trap Mass Spectrometer

描述（由申请人提供）：本申请的目的是向NCRR共享仪器计划申请购买LCQ Deca XP离子阱质谱仪的资金，该质谱仪完全集成并优化了蛋白质组学。这个ProteomeX工作站将是我们新成立的基因组学和蛋白质组学设施（GPF）的重要补充，GPF是俄勒冈大学生物科学小组的核心设施。生物科学小组由来自分子生物学研究所、神经科学研究所和分子进化小组的研究人员组成。质谱已成为蛋白质和大分子复合物表征的重要工具，随着人类、酵母、果蝇、秀丽隐杆线虫、神经孢子虫、斑马鱼以及大量细菌物种的基因组序列的完成，质谱已成为一种特别强大的工具。12个主要的研究小组已经描述了具体的项目，这些项目将受益于LCQ的使用，更具体地说，LC/MS/MS分析复杂的蛋白质混合物。主要用户将是：Tom Stevens， Roderick Capaldi, Frederick Dahlquist, Brian Matthews, Chris Doe, Alice Barkan, George Sprague, Beatrice Darimont, Bruce bowman, Eric Selker，以及我们的两位新教员Ken Prehoda和Andy Berglund。次要用户包括：Peter von Hippel， Jim Remington, Bill Roberts, Diane Hawley， Eric Johnson和Karen Guillemin。在俄勒冈大学看到质谱仪将使我们的研究人员能够立即使用这项技术，而不必依赖于远距离合作。

DESCRIPTION (provided by applicant): The purpose of this application to the NCRR Shared Instrumentation Program is to request funds for the purchase of an LCQ Deca XP ion trap mass spectrometer, fully integrated and optimized for proteomics. This ProteomeX Workstation will be an important addition to our newly formed Genomics and Proteomics Facility (GPF), which is a core facility serving the Biosciences Group at the University of Oregon. The Biosciences Group is made up of researchers from the Institute of Molecular Biology, the Institute of Neuroscience, and the Molecular Evolution Group. Mass spectrometry has become an essential tool in the characterization of proteins and macromolecular complexes, and has become a particularly powerful tool with the completion of the genome sequences for a wide variety of organisms, such as human, yeast, fruit fly, C. elegans, Neurospora, zebra fish, as well as a large number of bacterial species. Twelve major research groups have described specific projects that will benefit from the use of the LCQ and, more specifically, LC/MS/MS analysis of complex mixtures of proteins. The major users will be: Tom Stevens, Roderick Capaldi, Frederick Dahlquist, Brian Matthews, Chris Doe, Alice Barkan, George Sprague, Beatrice Darimont, Bruce Bowerman, Eric Selker, as well as two of our newest faculty Ken Prehoda and Andy Berglund. Minor users will include: Peter von Hippel, Jim Remington, Bill Roberts, Diane Hawley, Eric Johnson, and Karen Guillemin. The sighting of this mass spectrometer at the University of Oregon will make this technology immediately accessible to our researchers, rather than having to rely on long distance collaborations.