LCQ Deca XP Ion Trap Mass Spectrometer

描述（由申请人提供）：本申请的目的是向NCRR共享仪器计划申请资金，用于购买LCQ Deca XP离子阱质谱仪，该质谱仪完全集成并针对蛋白质组学进行了优化。这个ProteomeX工作站将是我们新成立的基因组学和蛋白质组学设施（GPF）的重要补充，GPF是服务于俄勒冈州大学生物科学组的核心设施。生物科学组由来自分子生物学研究所、神经科学研究所和分子进化组的研究人员组成。质谱已成为蛋白质和大分子复合物表征的重要工具，并且随着各种生物体（如人类、酵母、果蝇、C.线虫、脉孢菌、斑马鱼以及大量的细菌物种。十二个主要的研究小组已经描述了将受益于LCQ的使用的具体项目，更具体地说，LC/MS/MS分析蛋白质的复杂混合物。主要用户将是：汤姆·史蒂文斯，罗德里克·卡帕尔迪，弗雷德里克·达尔奎斯特，布赖恩马修斯，克里斯·多伊，爱丽丝·巴肯，乔治斯普拉格，比阿特丽斯·达里蒙，布鲁斯鲍尔曼，埃里克·塞尔克，以及我们的两个最新的教师肯·普雷霍达和安迪·伯格伦德。次要用户包括：Peter von Hippel、Jim Remington、Bill Roberts、Diane Hawley、Eric约翰逊和Karen Guillemin。在俄勒冈州大学看到这种质谱仪将使我们的研究人员立即获得这项技术，而不必依赖于长距离合作。

DESCRIPTION (provided by applicant): The purpose of this application to the NCRR Shared Instrumentation Program is to request funds for the purchase of an LCQ Deca XP ion trap mass spectrometer, fully integrated and optimized for proteomics. This ProteomeX Workstation will be an important addition to our newly formed Genomics and Proteomics Facility (GPF), which is a core facility serving the Biosciences Group at the University of Oregon. The Biosciences Group is made up of researchers from the Institute of Molecular Biology, the Institute of Neuroscience, and the Molecular Evolution Group. Mass spectrometry has become an essential tool in the characterization of proteins and macromolecular complexes, and has become a particularly powerful tool with the completion of the genome sequences for a wide variety of organisms, such as human, yeast, fruit fly, C. elegans, Neurospora, zebra fish, as well as a large number of bacterial species. Twelve major research groups have described specific projects that will benefit from the use of the LCQ and, more specifically, LC/MS/MS analysis of complex mixtures of proteins. The major users will be: Tom Stevens, Roderick Capaldi, Frederick Dahlquist, Brian Matthews, Chris Doe, Alice Barkan, George Sprague, Beatrice Darimont, Bruce Bowerman, Eric Selker, as well as two of our newest faculty Ken Prehoda and Andy Berglund. Minor users will include: Peter von Hippel, Jim Remington, Bill Roberts, Diane Hawley, Eric Johnson, and Karen Guillemin. The sighting of this mass spectrometer at the University of Oregon will make this technology immediately accessible to our researchers, rather than having to rely on long distance collaborations.