LCQ Deca XP Ion Trap Mass Spectrometer

描述(由申请人提供)：本申请NCRR共享仪器计划的目的是申请资金购买一台完全集成和优化蛋白质组学的LCQ Deca XP离子陷阱质谱仪。ProteomeX工作站将是我们新成立的基因组学和蛋白质组学设施(GPF)的重要补充，GPF是为俄勒冈大学生物科学组服务的核心设施。生物科学小组由来自分子生物学研究所、神经科学研究所和分子进化小组的研究人员组成。质谱学已经成为表征蛋白质和大分子复合体的重要工具，并随着人类、酵母、果蝇、线虫、脉孢子虫、斑马鱼以及大量细菌物种基因组序列的完成而成为一种特别强大的工具。12个主要研究小组描述了将受益于LC Q的使用的具体项目，更具体地说，将受益于复杂蛋白质混合物的LC/MS/MS分析。主要用户将是：Tom Stevens、Roderick Capaldi、Frederick Dahlquist、Brian Matthews、Chris Doe、Alice Barkan、George Sprague、Beatrice Darimont、Bruce Bowerman、Eric Selker以及我们的两位最新教员Ken Prehoda和Andy Berglund。未成年用户将包括：彼得·冯·希佩尔、吉姆·雷明顿、比尔·罗伯茨、黛安·霍利、埃里克·约翰逊和凯伦·吉列曼。在俄勒冈大学发现这种质谱计将使我们的研究人员立即获得这项技术，而不是必须依赖远程合作。

DESCRIPTION (provided by applicant): The purpose of this application to the NCRR Shared Instrumentation Program is to request funds for the purchase of an LCQ Deca XP ion trap mass spectrometer, fully integrated and optimized for proteomics. This ProteomeX Workstation will be an important addition to our newly formed Genomics and Proteomics Facility (GPF), which is a core facility serving the Biosciences Group at the University of Oregon. The Biosciences Group is made up of researchers from the Institute of Molecular Biology, the Institute of Neuroscience, and the Molecular Evolution Group. Mass spectrometry has become an essential tool in the characterization of proteins and macromolecular complexes, and has become a particularly powerful tool with the completion of the genome sequences for a wide variety of organisms, such as human, yeast, fruit fly, C. elegans, Neurospora, zebra fish, as well as a large number of bacterial species. Twelve major research groups have described specific projects that will benefit from the use of the LCQ and, more specifically, LC/MS/MS analysis of complex mixtures of proteins. The major users will be: Tom Stevens, Roderick Capaldi, Frederick Dahlquist, Brian Matthews, Chris Doe, Alice Barkan, George Sprague, Beatrice Darimont, Bruce Bowerman, Eric Selker, as well as two of our newest faculty Ken Prehoda and Andy Berglund. Minor users will include: Peter von Hippel, Jim Remington, Bill Roberts, Diane Hawley, Eric Johnson, and Karen Guillemin. The sighting of this mass spectrometer at the University of Oregon will make this technology immediately accessible to our researchers, rather than having to rely on long distance collaborations.