Acquiring robotic support for mass spectrometry core

描述（由申请人提供）：位于斯托尼布鲁克的大分子分析与合成中心（CASM）要求提供资金，以获得一个蛋白质组学机器人工作站，用于处理凝胶塞以进行质谱分析。该机构已经获得了ABI Voyager研究级MALDI质谱仪和Micromass QToF串联质谱仪，以支持斯托尼布鲁克的蛋白质组学研究。本申请寻求对Micromass MassPREP工作站的获得的支持，以提供用于详细质谱的凝胶塞的自动化96孔格式消化和点样/取样。有五个主要用户需要高通量容量来支持他们的NIH资助的研究。Danial Bogeneous博士（药理学）将在线粒体蛋白质组学中使用仪器，这是一个非常感兴趣的领域，旨在定义在mtDNA维护中起作用的蛋白质的完整补充。Wen-Tien Chen博士（医学-肿瘤学）需要仪器来支持他在血管生成和转移中涉及的侵入性细胞突起的蛋白质组学方面的工作。Malbon博士（药理学）正在使用蛋白质组学来推断G蛋白偶联受体以及β-连环蛋白/Lef-Tcf通路的大分子信号复合物的组成。托德米勒（生理学）需要这种仪器在他的酪氨酸激酶的蛋白质组学分析，重点是SH 3依赖的合作伙伴的HCK和相互作用的蛋白质合作伙伴的Jak 2。William货车Nostrand（医学-血管生物学）正在探索淀粉样β蛋白前体的加工以及使用蛋白质组学鉴定新的蛋白质-蛋白质相互作用。所有这些研究人员都发表了基于蛋白质化学和质谱的研究。为了优化仪器的使用，新的工作站将位于CASM，这是一个拥有十多年经验的蛋白质核心设施。已招聘了三名全职工程师来管理蛋白质组学中采用的质谱法。机构的承诺包括对这3名全职员工的持续支持，以及提供资金，以购买由医学院和研究副校长联合使用的辅助自动凝胶扫描仪/点选器。研究提案是高质量的，由NIH资助，并将受益于增加这种仪器。该机构提供了资源，以购买两台新的质谱仪，这项建议是在投资的基础上提出的。

DESCRIPTION (provided by applicant): The Center for Analysis & Synthesis of Marcomolecules (CASM) at Stony Brook requests funding to acquire a robotic workstation for proteomics to process gel plugs for analysis by mass spectrometry. The institution has acquired an ABI Voyager research-grade MALDI mass spectrometer and a Micromass QToF tandem mass spectrometer to support proteomics research at Stony Brook. The present application seeks support for the acquisition of a Micromass MassPREP workstation to provide an automated, 96-well format digestion and spotting/sampling of gel plugs for detailed mass spectrometry. There are five major users who need the high-throughput capacity to enable their NIH-funded research. Dr. Danial Bogenhagen (Pharmacology) will employ the instrumentation in mitochondrial proteomics, an area of high interest, seeking to define the full complement of proteins that act in maintenance of mtDNA. Dr. Wen-Tien Chen (Medicine-Oncology) requires the instrumentation to enable his work on the proteomics of invasive cell protrusions involved in angiogenesis and metastasis. Dr. Malbon (Pharmacology) is using proteomics to deduce the composition of large macromolecular signaling complexes for G-protein-coupled receptors as well as in beta-catenin/Lef-Tcf pathways. Todd Miller (Physiology) requires this instrumentation in his proteomic analysis of tyrosine kinases, focusing upon the SH3-dependent partners of Hck and interacting protein partners for Jak2. William Van Nostrand (Medicine-Vascular Biology) is probing the processing of amyloid beta-protein precursor as well as the identification of new protein-protein interactions using proetomics. All of these investigators have published research based in protein chemistry and in mass spectrometry. To optimize the use of the instrumentation, the new workstation will be located in CASM, a protein core facility with more than a decade of experience. Three FTEs have been recruited to manage the mass spectrometry employed in proteomics. The institutional commitment includes the on-going support for these 3 FTEs as well as provision of funds to acquire a complementary automated gel scanner/spot picker jointly by the School of Medicine and the Vice-President for Research. The research proposals are high quality, well-funded by the NIH, and will benefit by the addition of this instrumentation. The institution has provided resources to enable the acquisition of two new mass spectrometers and this proposal builds on the investment.