Acquiring robotic support for mass spectrometry core

描述（由申请人提供）：石溪大学马科分子分析与合成中心（CASM）申请资助，以获得一个蛋白质组学机器人工作站，用于处理凝胶塞，用于质谱分析。该机构已经获得了ABI Voyager研究级MALDI质谱仪和Micromass QToF串联质谱仪，以支持Stony Brook的蛋白质组学研究。目前的申请是为了获得Micromass MassPREP工作站的支持，以提供96孔格式的凝胶塞的自动消化和定位/取样，以进行详细的质谱分析。有五个主要用户需要高通量能力来支持他们的nih资助的研究。daniel Bogenhagen博士（药理学）将在线粒体蛋白质组学中使用该仪器，这是一个高度感兴趣的领域，旨在定义维持mtDNA的蛋白质的完整补体。陈文天（医学肿瘤学）博士需要该仪器来进行血管生成和转移过程中浸润性细胞突起的蛋白质组学研究。Malbon博士（药理学）正在使用蛋白质组学来推断g蛋白偶联受体以及β -连环蛋白/左- tcf途径的大分子信号复合物的组成。Todd Miller（生理学）在酪氨酸激酶的蛋白质组学分析中需要这种仪器，重点研究Hck的sh3依赖性伙伴和Jak2的相互作用蛋白伙伴。威廉·范·诺斯特兰德（医学-血管生物学）正在探索淀粉样蛋白前体的加工过程，以及利用蛋白质组学识别新的蛋白质-蛋白质相互作用。所有这些研究人员都发表了基于蛋白质化学和质谱的研究。为了优化仪器的使用，新的工作站将位于CASM，这是一个拥有十多年经验的蛋白质核心设施。已经招募了三名fte来管理蛋白质组学中使用的质谱分析。院方承诺的拨款包括持续支援这3名全职教员，以及拨款购置由医学院与负责研究的副校长联合购置的辅助自动凝胶扫描仪/斑点采集器。这些研究建议是高质量的，由美国国立卫生研究院提供充足的资金，并将受益于该仪器的增加。该机构提供了资源，使其能够获得两台新的质谱仪，该提案建立在投资的基础上。

DESCRIPTION (provided by applicant): The Center for Analysis & Synthesis of Marcomolecules (CASM) at Stony Brook requests funding to acquire a robotic workstation for proteomics to process gel plugs for analysis by mass spectrometry. The institution has acquired an ABI Voyager research-grade MALDI mass spectrometer and a Micromass QToF tandem mass spectrometer to support proteomics research at Stony Brook. The present application seeks support for the acquisition of a Micromass MassPREP workstation to provide an automated, 96-well format digestion and spotting/sampling of gel plugs for detailed mass spectrometry. There are five major users who need the high-throughput capacity to enable their NIH-funded research. Dr. Danial Bogenhagen (Pharmacology) will employ the instrumentation in mitochondrial proteomics, an area of high interest, seeking to define the full complement of proteins that act in maintenance of mtDNA. Dr. Wen-Tien Chen (Medicine-Oncology) requires the instrumentation to enable his work on the proteomics of invasive cell protrusions involved in angiogenesis and metastasis. Dr. Malbon (Pharmacology) is using proteomics to deduce the composition of large macromolecular signaling complexes for G-protein-coupled receptors as well as in beta-catenin/Lef-Tcf pathways. Todd Miller (Physiology) requires this instrumentation in his proteomic analysis of tyrosine kinases, focusing upon the SH3-dependent partners of Hck and interacting protein partners for Jak2. William Van Nostrand (Medicine-Vascular Biology) is probing the processing of amyloid beta-protein precursor as well as the identification of new protein-protein interactions using proetomics. All of these investigators have published research based in protein chemistry and in mass spectrometry. To optimize the use of the instrumentation, the new workstation will be located in CASM, a protein core facility with more than a decade of experience. Three FTEs have been recruited to manage the mass spectrometry employed in proteomics. The institutional commitment includes the on-going support for these 3 FTEs as well as provision of funds to acquire a complementary automated gel scanner/spot picker jointly by the School of Medicine and the Vice-President for Research. The research proposals are high quality, well-funded by the NIH, and will benefit by the addition of this instrumentation. The institution has provided resources to enable the acquisition of two new mass spectrometers and this proposal builds on the investment.