Acquiring robotic support for mass spectrometry core

描述(由申请人提供)：位于石溪的大分子分析与合成中心(CASM)申请资金，以购买一个用于蛋白质组学的机器人工作站，以处理凝胶塞以进行质谱分析。该机构已经获得了一台ABI Voyager研究级MALDI质谱仪和一台微质量QToF串联质谱仪，以支持石溪的蛋白质组学研究。本申请寻求对获得MicroMassMassPREP工作站的支持，以提供自动化的96孔格式的消化和凝胶塞的点样/采样，以用于详细的质谱学。有五个主要用户需要高通量能力来支持他们由NIH资助的研究。Danial Bogagen博士(药理学)将在线粒体蛋白质组学中使用这种仪器，这是一个非常有兴趣的领域，试图确定在线粒体DNA维持中起作用的全部蛋白质。陈文天博士(医学-肿瘤学)需要这种仪器来支持他在侵袭性细胞突起的蛋白质组学方面的工作，这些细胞突起涉及血管生成和转移。Malbon博士(药理学)正在利用蛋白质组学推断G蛋白偶联受体以及β-连环蛋白/Lef-Tcf通路的大分子信号复合体的组成。托德·米勒(生理学)在他对酪氨酸激酶的蛋白质组学分析中需要这种工具，重点是HCK依赖SH3的伙伴和JAK2相互作用的蛋白质伙伴。William Van Nostrand(医学-血管生物学)正在探索淀粉样β蛋白前体的加工过程，以及利用蛋白质组学识别新的蛋白质-蛋白质相互作用。所有这些研究人员都发表了基于蛋白质化学和质谱学的研究。为了优化仪器的使用，新的工作站将设在CASM，这是一家拥有十多年经验的蛋白质核心设施。已经招募了三名FTE来管理蛋白质组学中使用的质谱学。机构承诺包括继续为这3个全职员工提供支持，以及提供资金，由医学院和负责研究的副校长共同购买一台配套的自动凝胶扫描仪/斑点选取器。这些研究提案质量很高，由美国国立卫生研究院提供了充足的资金，并将受益于这一仪器的增加。该机构提供了资源，以便能够购买两台新的质谱仪，而这项提议是在投资的基础上提出的。

DESCRIPTION (provided by applicant): The Center for Analysis & Synthesis of Marcomolecules (CASM) at Stony Brook requests funding to acquire a robotic workstation for proteomics to process gel plugs for analysis by mass spectrometry. The institution has acquired an ABI Voyager research-grade MALDI mass spectrometer and a Micromass QToF tandem mass spectrometer to support proteomics research at Stony Brook. The present application seeks support for the acquisition of a Micromass MassPREP workstation to provide an automated, 96-well format digestion and spotting/sampling of gel plugs for detailed mass spectrometry. There are five major users who need the high-throughput capacity to enable their NIH-funded research. Dr. Danial Bogenhagen (Pharmacology) will employ the instrumentation in mitochondrial proteomics, an area of high interest, seeking to define the full complement of proteins that act in maintenance of mtDNA. Dr. Wen-Tien Chen (Medicine-Oncology) requires the instrumentation to enable his work on the proteomics of invasive cell protrusions involved in angiogenesis and metastasis. Dr. Malbon (Pharmacology) is using proteomics to deduce the composition of large macromolecular signaling complexes for G-protein-coupled receptors as well as in beta-catenin/Lef-Tcf pathways. Todd Miller (Physiology) requires this instrumentation in his proteomic analysis of tyrosine kinases, focusing upon the SH3-dependent partners of Hck and interacting protein partners for Jak2. William Van Nostrand (Medicine-Vascular Biology) is probing the processing of amyloid beta-protein precursor as well as the identification of new protein-protein interactions using proetomics. All of these investigators have published research based in protein chemistry and in mass spectrometry. To optimize the use of the instrumentation, the new workstation will be located in CASM, a protein core facility with more than a decade of experience. Three FTEs have been recruited to manage the mass spectrometry employed in proteomics. The institutional commitment includes the on-going support for these 3 FTEs as well as provision of funds to acquire a complementary automated gel scanner/spot picker jointly by the School of Medicine and the Vice-President for Research. The research proposals are high quality, well-funded by the NIH, and will benefit by the addition of this instrumentation. The institution has provided resources to enable the acquisition of two new mass spectrometers and this proposal builds on the investment.