CORE--Shared Resources Cell Imaging and Histology

CORE--共享资源细胞成像和组织学

• 批准号: 6990465
• 负责人: BARBARA RANSCHT
• 金额: $ 21.42万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-28 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990465
• 关键词: bioimaging /biomedical imaging; biomedical equipment resource; biomedical facility; cell components; confocal scanning microscopy; digital imaging; flow cytometry; fluorescence recovery after photobleaching; fluorescence resonance energy transfer; histology; image processing; imaging /visualization /scanning; light microscopy; neoplasm /cancer; oncology; tissue /cell culture

DESCRIPTION (provided by applicant): The Cell Imaging and Histology Shared Resource provides the equipment and expertise for three types of services: 1) High resolution microscopic techniques to obtain information about the morphology of cells, and sophisticated imaging techniques to analyze the distribution of molecules and their interactions within their cellular environment; 2) Histological and immunohistological techniques to study the morphology of normal and pathological specimens and the distribution of molecules in tissues; and 3) electron and immunoelectron microscopy to analyze the ultrastructure of of cells and the association of molecules with subcellular organelles. The Resource operates four light micsroscopes for confocal and standard imaging and an electron microscope for ultrastructural analysis. Equipment and services are provided for tissue embedding, sectioning, laser capture dissection, staining, and microscopic analysis of specimens. The goals for the next five years include expansion of existing services and introduction of new services. An increased effort will be placed on assisting users with the design and set-up of experiments, including sample collection and processing. New advanced optical imaging strategies for visualizing molecular events in living cells will be implemented, such as: 1) Optical imaging based on the principle of fluorescence resonance energy transfer (FRET) and fluorescent lifetime imaging (FLIM); 2) Total internal reflection fluorescence microscopy (TIRF) to register real-time interactions and trafficking of fluorescent molecules, and to study the dynamics of protein-protein interactions and enzymatic reactions in live cells; and 3) Fluorescence recovery after photobleaching (FRAP) to examine the lateral diffusion and mobility of fluorophore-labeled molecules into an area that has been photobleached. Overall the Cell Imaging and Histology Shared Resource provides essential microscopic and histological services and will accommodate the increased use and requirement for new services in response to new research initiatives at the Cancer Center.

描述（由申请人提供）：细胞成像和组织学共享资源提供 用于三类服务的设备和专业知识：1）高分辨率显微技术，用于获取有关细胞形态的信息，以及复杂的成像技术，用于分析分子的分布及其在细胞环境中的相互作用； 2）组织学和免疫组织学技术，研究正常和病理标本的形态以及组织中分子的分布； 3）电子和免疫电子显微镜分析细胞的超微结构以及分子与亚细胞器的关联。该资源拥有四台用于共焦和标准成像的光学显微镜以及一台用于超微结构分析的电子显微镜。提供用于组织包埋、切片、激光捕获解剖、染色和标本显微分析的设备和服务。未来五年的目标包括扩展现有服务和引入新服务。我们将加大力度协助用户设计和设置实验，包括样本收集和处理。将实施用于可视化活细胞中分子事件的新的先进光学成像策略，例如：1）基于荧光共振能量转移（FRET）和荧光寿命成像（FLIM）原理的光学成像； 2）全内反射荧光显微镜（TIRF）来记录实时相互作用和 荧光分子的运输，并研究活细胞中蛋白质-蛋白质相互作用和酶反应的动力学； 3) 光漂白后荧光恢复 (FRAP)，用于检查荧光团标记分子进入光漂白区域的横向扩散和迁移率。 总体而言，细胞成像和组织学共享资源提供了必要的微观和组织学信息 组织学服务，并将适应新服务的增加使用和需求 response to new research initiatives at the Cancer Center.