CORE--Shared Resources Cell Imaging and Histology

CORE--共享资源细胞成像和组织学

• 批准号: 6990465
• 负责人: BARBARA RANSCHT
• 金额: $ 21.42万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-28 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990465
• 关键词: bioimaging /biomedical imaging; biomedical equipment resource; biomedical facility; cell components; confocal scanning microscopy; digital imaging; flow cytometry; fluorescence recovery after photobleaching; fluorescence resonance energy transfer; histology; image processing; imaging /visualization /scanning; light microscopy; neoplasm /cancer; oncology; tissue /cell culture

DESCRIPTION (provided by applicant): The Cell Imaging and Histology Shared Resource provides the equipment and expertise for three types of services: 1) High resolution microscopic techniques to obtain information about the morphology of cells, and sophisticated imaging techniques to analyze the distribution of molecules and their interactions within their cellular environment; 2) Histological and immunohistological techniques to study the morphology of normal and pathological specimens and the distribution of molecules in tissues; and 3) electron and immunoelectron microscopy to analyze the ultrastructure of of cells and the association of molecules with subcellular organelles. The Resource operates four light micsroscopes for confocal and standard imaging and an electron microscope for ultrastructural analysis. Equipment and services are provided for tissue embedding, sectioning, laser capture dissection, staining, and microscopic analysis of specimens. The goals for the next five years include expansion of existing services and introduction of new services. An increased effort will be placed on assisting users with the design and set-up of experiments, including sample collection and processing. New advanced optical imaging strategies for visualizing molecular events in living cells will be implemented, such as: 1) Optical imaging based on the principle of fluorescence resonance energy transfer (FRET) and fluorescent lifetime imaging (FLIM); 2) Total internal reflection fluorescence microscopy (TIRF) to register real-time interactions and trafficking of fluorescent molecules, and to study the dynamics of protein-protein interactions and enzymatic reactions in live cells; and 3) Fluorescence recovery after photobleaching (FRAP) to examine the lateral diffusion and mobility of fluorophore-labeled molecules into an area that has been photobleached. Overall the Cell Imaging and Histology Shared Resource provides essential microscopic and histological services and will accommodate the increased use and requirement for new services in response to new research initiatives at the Cancer Center.

描述（由申请人提供）：细胞成像和组织学共享资源提供 提供以下三种服务的设备和专业知识：1）获取细胞形态信息的高分辨率显微镜技术，以及分析分子分布及其在细胞环境中相互作用的先进成像技术; 2）研究正常和病理标本形态以及组织中分子分布的组织学和免疫组织学技术; 3）电镜和免疫电镜分析细胞超微结构及亚细胞器分子的结合。该资源操作四个光显微镜共聚焦和标准成像和电子显微镜进行超微结构分析。提供用于组织包埋、切片、激光捕获解剖、染色和标本显微镜分析的设备和服务。未来五年的目标包括扩大现有服务和推出新服务。将更加努力协助用户设计和安排实验，包括样本收集和处理。将实现活细胞分子事件可视化的新的先进光学成像策略，如：1）基于荧光共振能量转移（FRET）和荧光寿命成像（FLIM）原理的光学成像; 2)全内反射荧光显微镜（TIRF）记录实时相互作用， 荧光分子的运输，并研究蛋白质-蛋白质相互作用和活细胞中的酶促反应的动力学;和3）光漂白后的荧光恢复（FRAP），以检查荧光团标记的分子进入已被光漂白的区域的横向扩散和流动性。 总的来说，细胞成像和组织学共享资源提供了必要的显微镜和 组织学服务，并将适应新服务的增加使用和需求， 对癌症中心新研究计划的回应。